( Introduction ...)Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
| HOME >> BIOLOGY >> TECHNOLOGY |
Introduction
With the introduction of two new reversed-phase column types, TSKgel
ODS-100V and TSKgel ODS-100Z, excellent chromatographic performance and
selectivity can be achieved for a wide range of sample types. TSKgel ODS-100Z
and TSKgel ODS-100V are based on a spherical, five micron, silica gel
with 100 angstrom pores; each material is derivatized with difunctional
octadecylmethylsilane, followed by an exhaustive endcapping reaction.TSKgel
ODS-100Z contains approximately 20% carbon, while TSKgel ODS-100V has
a lower ligand density resulting in about 15% carbon, which in turn leads
to stronger retention of polar compounds.
The analysis of water- and lipid-soluble vitamins requires both hydrophilic and hydrophobic retention capabilities of the stationary phase. Also, the composition of the stationary phase must allow for stable retention times in the absence of organic solvents in the mobile phase. As demonstrated, simple and fast analysis methods for the determination water and lipid soluble vitamins are possible on the TSKgel ODS-100V and the TSKgel ODS-100Z columns.
Analysis of Water Soluble Vitamins
A mixture of six water soluble vitamins was analyzed on TSKgel ODS- 100V
and TSKgel ODS-100Z under isocratic conditions with 99% water- 1% acetonitrile,
acidified with 0.1% TFA to a pH of 2.0. At an optimized temperature of
40C good separation was obtained for all compounds in less than 10 minutes
(see Figure 1 ).
Both columns managed to separate all vitamins by baseline. Thiamine,
pyridoxal and pyridoxine were better separated on the more polar TSKgel
'"/>
Source:
Related biology technology :
1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
3. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
4. A Unique Dimension in Biological Purity
5. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
8. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
9. High-Fidelity PCR with a Novel Polymerase Mixture
10. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
11. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
| Copyright © 2003-2009 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |