Navigation Links
Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance

s followed by 10 cycles at 95C for 30 seconds (denaturation), 58C for 30 seconds (annealing), and 72C for 2 minutes; followed by 20 cycles at 95C for 30 seconds, 58C for 30 seconds, and 72C for 2 minutes plus an additional 5 seconds per cycle. The conditions for RT-PCR (Figure 4) were: (RoboCycler 96 temperature cycler) 1 cycle at 95C for 1 minute followed by 30 cycles at 95C for 1 minute (denaturation), 60C for 1 minute (annealing), and 68C for 1 minute (targets <1 kb) or 2 minutes per kb (targets >4 kb). For each cycling regimen, one final extension cycle of 72C for 7 minutes was performed (after 30 cycles).

PCR products were electrophoresed on 1 to 4% agarose/1X TBE gels. PCR products were visualized by ethidium bromide staining, and the gels were imaged using the Eagle Eye II still video system.

REFERENCES

  1. Borns, M. and Hogrefe, H. (2000) Strategies 13: 1-3.

  2. Barnes, W.M. (1994) Proc. Natl. Acad. Sci. 91: 2216-2220.

  3. Borns, M., et al. (1999) Strategies 12: 33-36.

  4. Landre, P.A., Gelfand, D.H., and Watson, R.M. (1995) In PCR Strategies. Academic Press, San Diego, pp. 3-16.

* U.S. Patent Nos. 5,545,552, 5,556,772, 5,866,395 and 5,948,663 and patents pending.


'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
3. A Unique Dimension in Biological Purity
4. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
5. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
6. Enhanced PCR Cloning System
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
10. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
11. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
Post Your Comments:
(Date:7/11/2014)... 11, 2014 BCC Research ( http://www.bccresearch.com ... POLYMERASE CHAIN REACTION (PCR) TECHNOLOGY: GLOBAL MARKETS , the ... reached nearly $140 billion in 2013. This is estimated ... five-year compound annual growth rate (CAGR) of 28.6%. , ... interesting and exciting possibilities within the broader PCR field. ...
(Date:7/11/2014)...  Researchers have pioneered a revolutionary new way ... called Virtual Finger, allows scientists to move through ... synapses using the flat surface of their computer ... studies orders of magnitude more efficient, saving time, ... many areas of experimental biology. The software and ...
(Date:7/10/2014)... July 10, 2014 Product and ... very low abundance and are often “lost in ... time-consuming. , Join presenters Dr. Rowel Tobias, Senior ... John Anders, Head of Quality at Nanotherapeutics, Inc., ... that can speed detection and quantitation while achieving ...
(Date:7/10/2014)... July 10, 2014 Terascala, the industry ... that Alan Swahn, a former vice president of product ... as vice president of marketing. Terascala’s software when combined ... NetApp create the highest performance and most reliable solutions ... to guide Terascala’s channel expansion and broaden its product ...
Breaking Biology Technology:Global Market for Digital Polymerase Chain Reaction Technology to Reach $490 Billion by 2018; Equipment Sector Accounts for the Fastest Growing Segment With 33% CAGR 2Global Market for Digital Polymerase Chain Reaction Technology to Reach $490 Billion by 2018; Equipment Sector Accounts for the Fastest Growing Segment With 33% CAGR 3Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 3Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Terascala Expands Executive Team 2Terascala Expands Executive Team 3
... Wisconsin , , Editors Note: The Wisconsin ... NorthStar Economics 60-page report titled, the NorthStar Guide to ... primer for Wisconsin companies seeking information about risk capital available ... to excerpt their report in four-installments on WTN over the ...
... WI Futurist William Gibson is often quoted ... evenly distributed yet. Many speakers at the first annual ... to make a point about the current state of healthcare ... of Wisconsin (MCW) . , ,Featured presenters at the conference ...
... Toni Sikes of Guild.com , , WTN: What's the ... Sikes: In my opinion, it will be the continuous ... technology and e-commerce companies lies in better connecting customers with ... and technology communities must improve every element that surrounds our ...
Cached Biology Technology:Community Development Venture Capital 2Community Development Venture Capital 3Community Development Venture Capital 4Community Development Venture Capital 5Community Development Venture Capital 6Networking of Healthcare Information 2The Next Big Thing in E-Commerce - Guild.com CEO comments on Wisconsin's technology vision 2
(Date:7/11/2014)... Canyon fire started near the City of Entiat on ... resources, the fire quickly grew to over 1,000 acres ... the fire is under investigation by the Washington Department ... with existing local resources in developing fire control strategies ... (USFS), Washington State Department of Natural Resources, (WA DNR), ...
(Date:7/11/2014)... Institute of Standards and Technology (NIST) need a ... they often prove that necessity is truly the ... for M. Lorna De Leoz and Stephen Stein, ... glycomics. Glycomics is the study of the abundant, ... to proteins and lipids and influence cellular processes, ...
(Date:7/11/2014)... two different ways that allow unprecedented experimental insights ... of oxygen molecules in photosynthesis. The two studies ... Nature Communications . , "The new knowledge will ... oxidation, which are key components for building artificial ... energy in fuels like hydrogen, ethanol or methanol," ...
Breaking Biology News(10 mins):Molecular snapshots of oxygen formation in photosynthesis 2
... Assistant Professor Frank J. Slack at Yale University has identified ... lung cancer, presenting new possibilities for diagnosis and treatment. The ... and Developmental Cell. , The oncogene Ras is out of ... over-expressed or activated by mutation. According to Slack, a member ...
... at the Marine Biological,Laboratory (MBL) has published ... that supports her hypothesis that, when combined, ... as BCE--can act synergistically to alter a ... have previously studied the effects,of these pollutants ...
... a longtime University of Chicago collaborator, Goldstein conducted an experiment ... these skinny, graceful fish can swim from salt water to ... prompt an equally drastic physiological change: Our cells would take ... and rapidly causing death. So how do skates do it? ...
Cached Biology News:Yale Scientists Find MicroRNA Regulates Ras Cancer Gene 2Clam embryo study shows pollutant mixture adversely affects nerve cell development 2Clam embryo study shows pollutant mixture adversely affects nerve cell development 3Sea skate experiment sheds light on human cell transport 2
...
Rabbit polyclonal to Reptin/TIP49B/RUVB2 ( Abpromise for all tested applications). Antigen: Synthetic peptide (Human) Entrez Gene ID: 10856 Swiss Protein ID: Q6FIB9...
Jak3 Antibody Ship: Hot Store: -20 C...
XPC Immunogen: XPC (NP_004619, 141 a.a. ~ 251 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Biology Products: