Navigation Links
Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance

s followed by 10 cycles at 95C for 30 seconds (denaturation), 58C for 30 seconds (annealing), and 72C for 2 minutes; followed by 20 cycles at 95C for 30 seconds, 58C for 30 seconds, and 72C for 2 minutes plus an additional 5 seconds per cycle. The conditions for RT-PCR (Figure 4) were: (RoboCycler 96 temperature cycler) 1 cycle at 95C for 1 minute followed by 30 cycles at 95C for 1 minute (denaturation), 60C for 1 minute (annealing), and 68C for 1 minute (targets <1 kb) or 2 minutes per kb (targets >4 kb). For each cycling regimen, one final extension cycle of 72C for 7 minutes was performed (after 30 cycles).

PCR products were electrophoresed on 1 to 4% agarose/1X TBE gels. PCR products were visualized by ethidium bromide staining, and the gels were imaged using the Eagle Eye II still video system.

REFERENCES

  1. Borns, M. and Hogrefe, H. (2000) Strategies 13: 1-3.

  2. Barnes, W.M. (1994) Proc. Natl. Acad. Sci. 91: 2216-2220.

  3. Borns, M., et al. (1999) Strategies 12: 33-36.

  4. Landre, P.A., Gelfand, D.H., and Watson, R.M. (1995) In PCR Strategies. Academic Press, San Diego, pp. 3-16.

* U.S. Patent Nos. 5,545,552, 5,556,772, 5,866,395 and 5,948,663 and patents pending.


'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
3. A Unique Dimension in Biological Purity
4. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
5. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
6. Enhanced PCR Cloning System
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
10. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
11. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
Post Your Comments:
(Date:5/15/2008)...ple Reached to Terminate Marketing Agreement with ...Most Favorable Terms, NAPA, Calif., May 15 /PRNew...d: SNKTY), a Life Sciences company engaged in the ...e of healthy aging, today announced,financial resu...reement in,principle to terminate its marketing ag...
(Date:5/15/2008)..., May 15 /- Signalife, Inc.... Mr. Lee B. Ehrlichman to,the company,s board of d...ector of Operations. Mr. Ehrlichman, who pioneered...and call center monitoring as CEO and,President of...pany,operations as the company,s Operations Direct...
(Date:5/15/2008)... /- Cadus Corporation (OTC,...sults for the first,quarter ended March 31, 2008.,...,000, compared to,$100,000 for the same period in ...207,996, compared to net loss of $558,469 for the ...be attributed to a decrease in general,and adminis...
(Date:5/15/2008)...CELONA, Spain, May 15 /- Fo...orios Almirall, S.A. (ALM.MC),will host a webcast ...nical,data findings presented at the American Thor...ill include a slide presentation and a Q&A session...New York time, 3:30pm CET,2:30pm UKT., (Logo: h...
Breaking Biology Technology:Senetek PLC Reports First Quarter 2008 Financial Results 2Senetek PLC Reports First Quarter 2008 Financial Results 3Signalife Appoints Lee Ehrlichman as Company Director and Operations Director; Additional Notice Received From Amex Regarding Sharholders' Equity Deficiency 2Signalife Appoints Lee Ehrlichman as Company Director and Operations Director; Additional Notice Received From Amex Regarding Sharholders' Equity Deficiency 3Signalife Appoints Lee Ehrlichman as Company Director and Operations Director; Additional Notice Received From Amex Regarding Sharholders' Equity Deficiency 4Cadus Reports First Quarter 2008 Results 2Cadus Reports First Quarter 2008 Results 3Forest Laboratories, Inc. and Laboratorios Almirall, S.A. to Host Webcast to Discuss Aclidinium Bromide Data Presentations at the American Thoracic Society Annual Congress 2
...a Raton, FL - MDVIP, Inc. , named former Wiscon...Services Tommy Thompson as Chairman of the MDVIP C...thcare. , ,MDVIP is a provider of personalized hea...4 states. Member physicians limit their practices ...mary care practice of 2,500 patients, and focus on...
...arch engine experts have been hounding Google to a... their site information. When Google recently intr... showed experts that they have been listening - at...l feature for large shopping sites and other sites...nsures that the search engine result listings rela...
...ison, Wis. - Anyone who has listened to the const...too long knows how blazing hot computers can get. ...rate so much heat that air cooling technology can,...ngineering professor Tim Shedd . , ,Shedd and g...ray cooling method that early tests show can remov...
...st weeks column spurred enough e-mail to suggest t...dependence from technology (sort of). , ,As one re... technology is as impossible. (He also suggested i...t up to hyperbolic use of a metaphor.) , ,Im not s...just wont go back in the bottle and we wouldnt wa...
Other Biology Technology:Givin' it to Google: Using an XML Sitemap to Boost Your Site 2Givin' it to Google: Using an XML Sitemap to Boost Your Site 3A new tool for keeping computers cool 2A new tool for keeping computers cool 3Lamenting the loss of leisure 2Lamenting the loss of leisure 3
(Date:5/14/2008)...less Module Combines Linux, Security, and Low Powe...N, Calif., May 14 /PRNewswire/ -- Silex Technology...erformance module designed for connecting,devices ...wireless local,area networks (WLANs). It is ideal ...ivity with minimal power usage, such as battery-op...
(Date:5/14/2008)...gon white oak were once widespread in the Pacific ...in decline. Fire suppression, conifer and invasive...lted in the loss of as much as 99 percent of the o...of the region. , A new technical report titled "...Oak at Tenalquot Prairie, Washington" outlines the...
(Date:5/14/2008)...vailable in Spanish . , A scientific group ...A), Hawaii (USA) and Nakai (China), among others, ...equence, in this case papaya (Carica papaya), acco...gious journal Nature . One of the researchers is ...etics of the Department of Genetics of the Uni...
(Date:5/14/2008)...na. Scientists of the Helmholtz Centre for Environ...a Institute of Technology (Caltech) in Pasadena su...gether") microorganisms that are known to dramatic...he atmosphere. These microorganisms that oxidize m... the global carbon cycle and a major sink for meth...
Breaking Biology News(10 mins):New Silex SX-560 Enables Enterprise 802.11 Wireless Connectivity for Battery Operated Devices 2Scientists decipher fruit tree genome for the first time 2Scientists decipher fruit tree genome for the first time 3Deep sea methane scavengers captured 2Deep sea methane scavengers captured 3PepPods Helped Katrina Victims and Now Available to Help California Wildfire Disaster Victims Rebuild Lives with Greater Ease 4606 1PepPods Helped Katrina Victims and Now Available to Help California Wildfire Disaster Victims Rebuild Lives with Greater Ease 4606 2States Largest Medicaid Managed Care Plan Offering Training to Combat West Virginia Obesity Epidemic 4601 1States Largest Medicaid Managed Care Plan Offering Training to Combat West Virginia Obesity Epidemic 4601 2States Largest Medicaid Managed Care Plan Offering Training to Combat West Virginia Obesity Epidemic 4601 3Novocell Appoints Orville G Kolterman M D to Board of Directors 1505 1Novocell Appoints Orville G Kolterman M D to Board of Directors 1505 2Dr Yu Yamaguchi named recipient of the Humanitarian Scientific Achievement Award 4598 1
...e asthma attacks share a genetic profile that appe... new study by researchers at Cincinnati Children,s...door to the possibility of designing treatments sp...e severest forms of asthma. , The findings appear...d Clinical Immunology. , The study is based on an...
...ay to throw a wrench in the transmissions of sever... at Washington University School of Medicine in St...rotein that could help them develop drugs to stop ...es known as apicomplexans. , Results of the study ...f the National Academy of Sciences. Scientists ide...
...pkins researchers have discovered that the antibio...e effects on the brain and central nervous system,...ral therapy controls the virus elsewhere in the bo...iciency virus (SIV), a very close relative of HIV,...rain cells, less brain inflammation, and less viru...
... gene influences taste sensitivity of children and...taste preferences and food selection, report a tea...enter. In addition to genes, age and culture also ...ng the influence of genetics. , The findings may ...ed to sweet-tasting foods, as well as why taste an...
Other Biology News:Asthma gene clusters identified 2Asthma gene clusters identified 3Protein offers way to stop microscopic parasites in their tracks 2Protein offers way to stop microscopic parasites in their tracks 3Antibiotic might fight HIV-induced neurological problems 2Antibiotic might fight HIV-induced neurological problems 3Children's taste sensitivity and food choices influenced by taste gene 2Children's taste sensitivity and food choices influenced by taste gene 3
Request Info
...ent kit contains the basic components required for...ural and recombinant rat Thymus Chemokine-1 in cel...sufficient materials to run ELISAs on approximatel...ing conditions are met: The assay is run as summ...
Conjugation services for Antibodies, Proteins, Oligos, Peptides, Beads, bioconjugation, antibody-oligonucleotide, LNA peptide, LNA protein, LNA antibody, HRP, Alkaline phosphatase, phycoerythrin, FIT
Blimp-1 (H-150)
Biology Products: