Navigation Links
Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance

other PCR application requirements. The use of Herculase DNA polymerase overcomes performance limitations of single-enzyme formulations (e.g. Taq, Platinum Pfx), while eliminating the need for multiple specialized DNA polymerase blends.

Methods

PCR amplifications (50 l) employed 200 M each dNTP (or 300 M each dNTP for Platinum Pfx), 100 ng of each primer, and 100 ng of genomic DNA. As recommended by the enzyme manufacturers, amplifications were carried out in each enzymes recommended buffer using 2.5U of Herculase, AmpliTaq (Applied Biosystems), Vent (NEB), or Deep Vent (NEB) DNA polymerase or 1.25U of Platinum Pfx DNA polymerase. For GC-rich PCR targets, amplifications were carried out using 2.5U of Herculase DNA polymerase, 5U to 6U of AdvantTaq Plus (CLONTECH), or 2U of GC-RICH PCR System DNA polymerase and each enzymes recommended buffer and PCR additive solution.

Reactions were cycled in 200-l thin-walled PCR tubes, using a RoboCycler Gradient 96 Temperature Cycler (Stratagene) or a single-block thermocycler, fitted with a Hot Top device. Enzyme comparisons employed identical temperature cycling parameters. For PCRs of genomic DNA targets (Figure 1, Figure 2, Figure 3 and Figure 6), the following conditions were used: (single-block cycler) 1 cycle at 95C for 1 minute followed by 30 cycles at 95C for 30 seconds (denaturation), 58C for 30 seconds (annealing), and 72C for 40 seconds (<900 bp) or 1 minute (900 bp) or 2 minutes (>1 kb). The conditions used to amplify the GC-rich amplicon (Figure 5) were as follows: (RoboCycler 96 temperature cycler) 1 cycle at 98C for 3 minutes followed by 30 cycles at 95C for 1 minute (denaturation), 58C for 40 seconds (annealing), and 72C for 2 minutes; (single-block cycler) 1 cycle at 95C for 3 minute
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
3. A Unique Dimension in Biological Purity
4. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
5. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
6. Enhanced PCR Cloning System
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
10. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
11. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
Post Your Comments:
(Date:12/24/2014)... “Preparative & Process Chromatography Market by Instrument ... Buffers, Valves, Guages, Seals), Accessories, Services, End User ... 2019” provides a detailed overview of the major ... strategies impacting the preparative and process chromatography market ... revenue and share analysis. , Full Copy ...
(Date:12/24/2014)... Bethesda, MD (PRWEB) December 23, 2014 ... signed into law H.R. 83, the Omnibus and ... the first time, made hydrocephalus a condition eligible ... Research Programs (CDMRP) administered by the Department of ... conjunction with its allies on Capitol Hill, is ...
(Date:12/24/2014)... The report expects global cell isolation and expansion market ... a carefully analyzed data about the vital drivers, restraints, ... Full Copy of Report @ http://bit.ly/1yUxy0T , ... expansion will keep witnessing growth at an impressive CAGR ... driven by rapid technological advancements, government funding and investments, ...
(Date:12/24/2014)... December 23, 2014 PharmaBoardroom,s ... out today and available for free download , digs ... change and growth in the sector today. One ... successes has been in developing a homegrown pharmaceutical manufacturing base, ... production still remains some way off. A cursory comparison with ...
Breaking Biology Technology:Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 3PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3
... America,s war on terror includes fighting the dark ... chemist Dr. Frank Raushel is helping with the fight ... chemical agent, the organophosphates. , Synthetic organophosphates started their ... their way into the dangerous alleys of chemical warfare ...
... away, but soccer aficionados can get an early start ... National Institute of Standards and Technology (NIST) and the ... summer. , Nanosoccerthe Lilliputian competition where computer-driven "nanobots" the ... no bigger than a grain of ricewill be part ...
... SAN DIEGO, Nov. 13 Gen-Probe Incorporated,(Nasdaq: GPRO ... the Lazard,Capital Markets 5th Annual Healthcare Conference on Tuesday, ... presentation is scheduled,to be webcast live and may be ... website at http://www.gen-probe.com . The webcast,will be available ...
Cached Biology Technology:Researcher working on destruction of chemical weapons 2Let the games begin! Nanosoccer at 2009 RoboCup in Austria 2Gen-Probe to Webcast Presentation at the Lazard Capital Markets 5th Annual Healthcare Conference 2
(Date:12/17/2014)... Dec. 15, 2014  HITLAB SM announced ... (GCP) audit to confirm its adherence to current ... accomplishment enables HITLAB to conduct regulated smart device ... principles for patient safety and research quality. ... access, quality, and delivery with innovative technology," said ...
(Date:12/17/2014)... Research and Markets ( http://www.researchandmarkets.com/research/9zq33j/global_chemical ... "Global Chemical Sensor Market 2015-2019" report to ... One major trend upcoming in ... sensors in biomedical applications. Chemical sensors help in ... diagnosis during surgical procedures. The Global Chemical Sensor ...
(Date:12/15/2014)... DUBLIN , Dec. 12, 2014 Research ... announced the addition of the "Global Facial ... http://photos.prnewswire.com/prnh/20130307/600769 ... the identification of individuals. Facial recognition system measures ... as nose, jaw edges, mouth, and the distance ...
Breaking Biology News(10 mins):HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3
... have found that melatonin, a naturally occurring brain substance, ... as seasonal affective disorder, or SAD. The study is ... National Academy of Science. , The study was led ... in the study of circadian (24-hour) rhythm disturbances, such ...
... and infects the most common and deadly form of brain ... devour themselves, researchers at The University of Texas M. D. ... the National Cancer Institute. , The modified adenovirus homed in ... among the cancer cells -- a process called autophagy -- ...
... the neural machinery that triggers the hungry brain to ... swings the other way after a hearty meal, as ... siesta. However, scientists have understood little about how the ... energy-related behaviors. , Now, in an article in the ...
Cached Biology News:Melatonin improves mood in winter depression 2Targeted virus compels cancer cells to eat themselves 2Targeted virus compels cancer cells to eat themselves 3New mechanism explains glucose effect on wakefulness 2New mechanism explains glucose effect on wakefulness 3
For removal of the zona pellucida. Sterile, Mouse Embryo tested...
... linear ion trap mass spectrometer enables high-throughput ... The LXQ linear ion trap mass spectrometer ... applications, delivering the fast cycle time and ... trap at an attractive price. The ...
... enables scientists who do not have access to ... have their biological samples tested for the concentration ... as 1-2-3 Chose the analytes you ... the S&S antibody menu. Send your samples ...
... 2) Dot plot, Contour plot, Density ... 4) Cell Cycle 5) Analysis ... batch generation of graphs and statistics 8) ... operations to perform complex batch analyses 10) ...
Biology Products: