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Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance

Vent) or greater than (Platinum Pfx, KOD) the fidelity of all commercial proofreading DNA polymerases except Pfu and PfuTurbo DNA polymerases (Figure 7). Additional comparisons show that Herculase DNA polymerase also exhibits significantly greater accuracy than Taq and Taq-based DNA polymerase blends, including blends formulated specifically for high-fidelity and long PCR applications.1

Fig.7

Conclusions

As demonstrated here and previously,1 Herculase enhanced DNA polymerase excels in a broad range of PCR applications. Herculase DNA polymerase can be readily used in procedures normally carried out with Taq DNA polymerase to improve reliability of PCR amplification reactions. In addition to producing higher product yields, Herculase DNA polymerase is also superior to Taq in difficult PCR applications, such as multiplexing, amplifying GC-rich or longer complex targets, amplifying cDNA, and producing high product yields from limiting amounts of DNA template. Moreover, superior PCR performance is achieved with high fidelity.

Proofreading DNA polymerases and Taq-based DNA polymerase blends are routinely used for high-fidelity PCR amplification. Comparisons show that Herculase DNA polymerase provides higher product yields, compared to proofreading DNA polymerases, and broader target-length capability and better amplification of extra-long targets (>20 kb) compared to DNA polymerase blends.1 Moreover, superior PCR performance is achieved with comparable or greater accuracy. Only the fidelity of Pfu and PfuTurbo DNA polymerases is higher than that of Herculase DNA polymerase.

While PfuTurbo remains the best choice for routine PCR applications demanding the highest fidelity possible, Herculase DNA polymerase excels in all
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