Navigation Links
Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance

A polymerase blend and varying concentrations (0 to 2 M) of GC-RICH resolution solution (betaine). Amplifications were performed with the GC-RICH PCR System using both the RoboCycler 96 temperature cycler (amplification unsuccessful, data not shown) and the single-block cycler recommended by the manufacturer (Figure 5).

Herculase DNA polymerase was also compared to the Advantage-GC 2 PCR kit (CLONTECH). Amplification was carried out as recommended by the kits manufacturer using a hot start Taq DNA polymerase blend, a PCR buffer containing 5% DMSO, and various concentrations of GC-Melt reagent (0 to 1.5 M). Despite repeated attempts, we were unsuccessful in amplifying the 300-bp target using the Advantage-GC 2 PCR kit (data not shown).

Therefore, Herculase DNA polymerase can be used with the provided DMSO to amplify difficult GC-rich targets, obviating the need for specialized DNA polymerase blends and PCR additives.

Multiplex PCR

Fig.6

Herculase enhanced DNA polymerase was also tested in multiplex PCR applications. In Figure 6, Herculase DNA polymerase provided uniform amplification of three genomic DNA targets (300 bp, 500 bp, 900 bp). Using the same reaction conditions, greater variation in amplicon yield was observed with Taq DNA polymerase (data not shown).

PCR Fidelity Comparisons

Herculase enhanced DNA polymerase exhibits high fidelity due to its novel pfu-based composition. In previous studies,1 Herculase DNA polymerase was found to exhibit an average error rate of 2.8 x 10-6 mutation frequency per base pair per duplication.1 Comparisons show that the fidelity of Herculase DNA polymerase is comparable to (Vent, Deep
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
3. A Unique Dimension in Biological Purity
4. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
5. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
6. Enhanced PCR Cloning System
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
10. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
11. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
Post Your Comments:
(Date:3/2/2015)... 2015 VG Life Sciences, Inc., (OTCQB: ... and infectious diseases, announced that the U.S. Patent ... new composition of matter patent covering methods for ... CLIP. U.S. Patent No. 8957031 ... Sciences, VG1177, a synthetic peptide which has the ...
(Date:3/2/2015)... Outside GC, the leading provider of on-demand, ... Debbie Casey as a member of the firm, further ... , Debbie has spent over 20 years in ... Care North America in both legal and business capacities. ... with Outside GC’s growing list of healthcare clients, with ...
(Date:2/27/2015)... 27, 2015 Immunovaccine Inc. (“Immunovaccine” or ... a fraudulent press release disseminated this morning by PR ... Immunovaccine. This press release states that Immunovaccine has entered ... The press release did not originate from Immunovaccine and ... the press release. Please note that all official ...
(Date:2/27/2015)... Mass. , Feb. 27, 2015 ... of the NCATS rare-diseases portfolio " in ... a new financing technique to reduce the risk ... diseases and potentially unlock new levels of funding ... The product of a unique collaboration between scientists ...
Breaking Biology Technology:VG Life Sciences Granted Key Composition Of Matter Patent 2VG Life Sciences Granted Key Composition Of Matter Patent 3Outside GC Expands Healthcare Team 2Immunovaccine Disclaims Hoax Press Release 2New Financing Technique May Hold Key to Unlocking Funding for Rare Diseases 2New Financing Technique May Hold Key to Unlocking Funding for Rare Diseases 3New Financing Technique May Hold Key to Unlocking Funding for Rare Diseases 4
... Odenheim, director of IT services at Synteract, Inc., ... on Selecting the Optimum EDC Platform to Support your ... East Coast conference , to be held in Philadelphia, March ... March 28, discussing a variety of drivers to consider when ...
... capacitors (ECs), also known as supercapacitors or ultracapacitors, differ ... TV or computer in that they store substantially higher ... storage devices as they charge and discharge faster than ... densities, only a fraction of the energy density of ...
... 2012 Sigma-Aldrich® Corporation (NASDAQ: SIAL ) ... Wuxi, China, that will provide localized packaging, analytical services ... Company,s research and industrial customers in the region. ... in industrial growth, and now we are viewing a ...
Cached Biology Technology:Synteract Director of IT Services Toby Odenheim Speaking at March 2012 Outsourcing in Clinical Trials East Coast Conference 2Synteract Director of IT Services Toby Odenheim Speaking at March 2012 Outsourcing in Clinical Trials East Coast Conference 3Researchers develop graphene supercapacitor holding promise for portable electronics 2Researchers develop graphene supercapacitor holding promise for portable electronics 3Researchers develop graphene supercapacitor holding promise for portable electronics 4Sigma-Aldrich Opens New Wuxi Packaging and Quality Control Facilities to Enhance Capabilities Serving China and Asia-Pacific Region 2Sigma-Aldrich Opens New Wuxi Packaging and Quality Control Facilities to Enhance Capabilities Serving China and Asia-Pacific Region 3
(Date:2/5/2015)... 3, 2015 Despite a lack of ... such as consumer electronics, automotive, storage and telecommunication ... technology (SMT) screen printers. Innovations in electronics and ... push the adoption curve up. Meanwhile, sale volumes ... glue dispensers are indispensable in the production of ...
(Date:1/22/2015)... , Jan. 13, 2015 /PRNewswire/ - Today, FindBiometrics, the leading ... the launch of its new website design. "When ... its infancy", says Peter O,Neill , founder and CEO ... the industry needs involvement from the key players on a ...
(Date:1/22/2015)... MADISON, Wis. , Jan. 15, 2015  BellBrook ... for drug discovery, announced the launch of a ... of the company,s Transcreener UDP Assay, a high ... enzymes. The new assay will allow for sensitive ...
Breaking Biology News(10 mins):End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 2End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 3Identity Industry Leading Publication Debuts New Look As Biometrics Hit Mainstream 2BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2
... marine data enables students to carry out scientifically valid ... knowledge is created and developed, according to research from ... University of Gothenburg followed upper-secondary students from the Swedish ... part of the research project I2I, Inquiry to Insight., ...
... 2012 Disruptive Science and Technology , a new ... Inc., publishers, has officially released its inaugural issue. ... Career Professor, Carnegie Mellon University, the Journal presents ... analyses focused on front-line concepts that will improve ...
... MO, May 14, 2012Arranging DNA fragments into a genome sequence ... assembling a puzzle except you don,t have the box and ... Sometimes clues from other publicly-available DNA sequences of related organisms ... usefulness depends on how closely related any two sequences are ...
Cached Biology News:Real science in virtual school labs 2New journal on disruptive science and technology launched by Mary Ann Liebert Inc. publishers 2Researchers look to relatives for clues in quest to develop sources of bioenergy 2Researchers look to relatives for clues in quest to develop sources of bioenergy 3
... microarrays are derived from frozen embedded tissue ... control procedures to ensure the highest quality. ... in one block, sectioned at a thickness ... slides. Sections are large (3-5 mm in ...
... tissue microarrays are derived from frozen embedded ... quality control procedures to ensure the highest ... assembled in one block, sectioned at a ... slides. Sections are large (3-5mm in diameter) ...
... are derived from Paraffin embedded tissue blocks, ... procedures to ensure the highest quality. Tissues ... one block, sectioned at a thickness of ... Sections are large (3-5mm in diameter) in ...
... Precision-designed for Optimal Microdissection , ... enable the precise and rapid extraction of ... and cytology samples with Laser Capture Microdissection., ... for Downstream Molecular Analysis , All CapSure ...
Biology Products: