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Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance

polymerase required at least 25 ng of template DNA. The superior sensitivity of Herculase DNA polymerase compared to Taq DNA polymerase was further demonstrated in template titration studies using additional primer sets (data not shown).

In Figure 3, a visible difference is observed in the migration of products synthesized by Taq DNA polymerase compared to Herculase DNA polymerase. Slight differences in band migration are the result of differences in PCR buffer composition and product yield (data not shown).

RT-PCR Performance Comparisons

Herculase enhanced DNA polymerase was compared to Taq DNA polymerase in RT-PCR reactions using a panel of targets ranging in length from 0.6 to 7.6 kb. Reverse transcription of total RNA was carried out with the reagents and protocol provided in the prostar Ultra HF RT-PCR system.3 Equivalent amounts of cDNA were then added to PCR reactions carried out with Herculase or Taq DNA polymerase in each enzymes recommended PCR buffer.

Fig.4

As shown in Figure 4, Herculase DNA polymerase consistently produced high yields of all cDNA targets up to 7.6 kb in length; however, Taq DNA polymerase could only amplify the 0.6-kb target in sufficient yield. In additional RT-PCR comparisons, Herculase DNA polymerase synthesized a higher yield of a 660-bp b-actin target compared to Taq DNA polymerase (data not shown).

Successful Amplification of GC-Rich Targets

Herculase enhanced DNA polymerase is provided with an optimized PCR reaction buffer and DMSO. As discussed previously,1 the addition of DMSO can increase product yield and extend the target-length capability of the Herculase formulation, while having minimal effects on fidelity. Although it is not
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