Navigation Links
Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance

compared to Taq DNA polymerase and to proofreading PCR enzymes in a variety of procedures, including RT-PCR, multiplex PCR, and amplifications employing GC-rich and complex genomic DNA targets.

PCR Yield and Target-Length Comparisons

Fig.1

Herculase enhanced DNA polymerase was compared to Taq DNA polymerase (Figure 1) and to proofreading DNA polymerases (Figure 2) in amplifications of genomic DNA targets less than 3 kb in length. PCRs were performed under identical conditions, using each enzymes recommended buffer (Methods).

Fig.2

Herculase DNA polymerase consistently produced high yields of all genomic DNA targets, ranging in length from 105 bp to 2.6 kb (Figure 1 and Figure 2). In contrast, Taq DNA polymerase synthesized each amplicon in lower yield, and recoveries decreased with increasing amplicon size for targets greater than 1 kb in length (Figure 1). Comparisons with proofreading DNA polymerases showed that even in PCRs of relatively short targets (105 to 500 bp), Herculase DNA polymerase produced significantly higher product yields than Vent, Deep Vent, and Platinum Pfx DNA polymerases (Figure 2).

PCR Sensitivity Comparisons

Fig.3

The sensitivity of Herculase enhanced DNA polymerase was demonstrated in PCR amplifications that employed limiting concentrations of genomic DNA template. In Figure 3, Herculase DNA polymerase successfully amplified a 2-kb amplicon using as little as 10 ng of genomic DNA. In contrast, successful amplification with Taq DNA
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
3. A Unique Dimension in Biological Purity
4. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
5. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
6. Enhanced PCR Cloning System
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
10. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
11. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
Post Your Comments:
(Date:10/22/2014)... 2014 Nuvilex, Inc. (OTCQB: NVLX) – According ... worldwide are living with diabetes, with  that number expected ... global market for diabetes treatments is approximately $500 billion.  ... died from pancreatic cancer.  Pancreatic cancer is the fourth ... the United States , and according ...
(Date:10/20/2014)... 20, 2014 The report “Pharmacy ... Systems, Packaging and Labeling Systems, Table-top Counters) by ... Global Forecasts & Trends to 2019” analyzes and ... in North America, Europe, Asia-Pacific, and the Rest ... tables and 30 figures spread through 300 pages ...
(Date:10/20/2014)... PARK, Calif. , Oct. 20, 2014 /PRNewswire/ ... today that the Company has signed a Notice ... of Regenerative Medicine (CIRM), effective October 1, 2014.  ... Clinical Development payments and the release of additional ... million CIRM grant award for clinical development of ...
(Date:10/20/2014)... 20, 2014  GenVec, Inc. (NASDAQ: GNVC ) ... Ph.D., from its board of directors effective on October 24, ... and served as its chairman from June 2006 to November ... and Corporate Governance and Audit Committees of the board.  ... decade of dedicated service to GenVec, and its stockholders," said ...
Breaking Biology Technology:Nuvilex Brief Analyst Report: Thinking Outside the Box by BrokerBank Securities, Inc. 2Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 2Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 3Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 4Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4Zola P. Horovitz To Retire From GenVec Board 2
... 24 The Charlotte-Mecklenburg region,certainly has not been immune ... NC Free Clinic Association has seen their highest,demand for ... creator of,Charlotte,s premier health & wellness event, the Charlotte ... , "We can all have ...
... Extensive Experience In Molecular DiagnosticsSALT LAKE CITY, Feb. ... spine care through the development of molecular diagnostic ... has joined the executive management team as the ... Dr. Macina will report directly to Kenneth Ward, ...
... Ore. Researchers have identified the gene that ultimately ... that could some day lead to the repair of ... restoration or even the production of replacement teeth. , ... was already known to have several functions - in ...
Cached Biology Technology:Charlotte Anti-Aging Expo & Symposium Supports Free Clinics 2Axial Biotech Appoints Dr. Roberto Macina to Vice President of Research and Development 2Genetic discovery could lead to advances in dental treatment 2
(Date:10/16/2014)... infections and worldwide claims the lives of 160,000 ... University have succeeded in developing a promising vaccination ... , Xavier Saelens (VIB/UGent): "We discovered a new ... development of a novel approach to vaccination against ... small children and elderly people." , RSV: ...
(Date:10/15/2014)... a non-traditional biophysics textbook and it describes how life ... of discovery into biological systems and provides an understanding ... is about how our genes make proteins that control ... cells in an organism. It quantifies the principles of ... be found on both large and small scales. The ...
(Date:10/15/2014)... 44th Rosenstiel Award for Distinguished Work in Biomedical Science ... mechanisms of genomic instability and its implications for the ... alumnus to win the Rosenstiel Award; the first, Rod ... is the Charles A. Janeway Professor of Pediatrics and ... investigator at the Howard Hughes Medical Institute at Boston ...
Breaking Biology News(10 mins):New perspectives for development of an RSV vaccine 2New book about life as seen from physics 2Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2
... turkeys died in Britain of cancer of the liver. Eventually, ... of aflatoxin as the cause of this mysterious 'turkey X' ... the most virulent natural carcinogenic substances. , The tasteless toxin ... hot, arid regions, in the south-west of the US and ...
... of people in southwest China suffer from potentially-crippling fluoride ... coal, say researchers. , The findings, from a study ... that the disease was caused by people burning fluoride-rich ... this belief, last year, the Chinese authorities invested 34 ...
... what many scientists thought, genetically modified (GM) corn has ... Mexico. , After analyzing tens of thousands of seeds ... from Mexico and the United States found no evidence ... surprised the researchers, said Allison Snow, a professor of ...
Cached Biology News:Corn grain mould used as pesticide 2Fluoride poisoning in China due to clay, not coal 2Genetically modified maize not found in southern Mexico 2
...
Request Info...
Human RUNX2/CBFA1 MAb (Clone 232902)...
... Peroxidase Conjugate, 2 ml. ... enzyme molecules for use ... situ hybridization applications.High specificity ... Labeling & Detection, Protein ...
Biology Products: