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Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance

compared to Taq DNA polymerase and to proofreading PCR enzymes in a variety of procedures, including RT-PCR, multiplex PCR, and amplifications employing GC-rich and complex genomic DNA targets.

PCR Yield and Target-Length Comparisons

Fig.1

Herculase enhanced DNA polymerase was compared to Taq DNA polymerase (Figure 1) and to proofreading DNA polymerases (Figure 2) in amplifications of genomic DNA targets less than 3 kb in length. PCRs were performed under identical conditions, using each enzymes recommended buffer (Methods).

Fig.2

Herculase DNA polymerase consistently produced high yields of all genomic DNA targets, ranging in length from 105 bp to 2.6 kb (Figure 1 and Figure 2). In contrast, Taq DNA polymerase synthesized each amplicon in lower yield, and recoveries decreased with increasing amplicon size for targets greater than 1 kb in length (Figure 1). Comparisons with proofreading DNA polymerases showed that even in PCRs of relatively short targets (105 to 500 bp), Herculase DNA polymerase produced significantly higher product yields than Vent, Deep Vent, and Platinum Pfx DNA polymerases (Figure 2).

PCR Sensitivity Comparisons

Fig.3

The sensitivity of Herculase enhanced DNA polymerase was demonstrated in PCR amplifications that employed limiting concentrations of genomic DNA template. In Figure 3, Herculase DNA polymerase successfully amplified a 2-kb amplicon using as little as 10 ng of genomic DNA. In contrast, successful amplification with Taq DNA
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