Navigation Links
Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance


Improve reliability of PCR reactions with Herculase enhanced DNA polymerase

Michael Borns Holly Hogrefe
Stratagene

Stratagene recently released Herculase enhanced DNA polymerase,*, a novel enzyme formulation that provides superior performance in both routine and demanding PCR applications. Previous comparisons showed that Herculase DNA polymerase exhibits greater fidelity than commercial DNA polymerase blends, in addition to producing higher product yields over an extremely broad range of target sizes (0.1 to 48 kb). In this follow-up article, we compared Herculase DNA polymerase to single-enzyme formulations and found it to be more reliable than Taq DNA polymerase in PCR applications; it provided higher product yield, greater sensitivity, and improved synthesis of difficult (long, multiplex, GC-rich) targets. Herculase DNA polymerase was also the superior performer when compared to proofreading PCR enzymes. Moreover, its accuracy was equal to or greater than the accuracy of all proofreading DNA polymerases except Stratagenes Pfu and PfuTurbo DNA polymerases. *,

Herculase enhanced DNA polymerase was developed to meet the need for a single PCR reagent for a broad range of PCR applications.1 Because of its unique Pfu-based composition, Herculase DNA polymerase has distinct properties that distinguish it from Taq-based DNA polymerase blends (formulated as described2). Previous comparisons to commercial DNA polymerase blends showed that Herculase DNA polymerase exhibited higher fidelity, broader target-length capability (0.1 to 48 kb), and superior amplification of extra-long targets (>20 kb).1

Hence, we tested Herculase DNA polymerase in PCR applications that are routinely carried out using single-enzyme formulations. The Herculase formulation was
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
3. A Unique Dimension in Biological Purity
4. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
5. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
6. Enhanced PCR Cloning System
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
10. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
11. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
Post Your Comments:
(Date:4/23/2015)... Six startups from Russia traveled ... as part of the U.S.-Russia Innovation Corridor (USRIC) ... biotechnology startups to participate in the program since ... the University of Maryland (UMD) signed a Memorandum ... universities in the biomedical industry in 2013. , ...
(Date:4/23/2015)... SAN FRANCISCO, Calif. , April 23, 2015  Veracyte, ... has entered into a definitive agreement to sell approximately ... placement. Under the agreement, the investors will purchase an ... purchase price of $8.15 per share, the closing stock ... led by new and existing investors, including Broadfin Capital, ...
(Date:4/23/2015)... 2015  Propanc Health Group Corporation (OTCBB: PPCH) ... the development of cancer treatments for patients with ... includes PRP, a patented formulation consisting of two ... as the enzyme amylase designed to synergistically enhance ... of anti-cancer agents working in combination with pro-enzymes, ...
(Date:4/22/2015)... -- Sangamo BioSciences, Inc. (NASDAQ: SGMO ) today reported ... For the first quarter ended March 31, 2015, ... or $0.08 per share, compared to a net loss ... same period in 2014. As of March 31, 2015, ... interest receivable of $226.1 million. Revenues for ...
Breaking Biology Technology:U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 2U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 3U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 4Veracyte, Inc. Announces $40 Million Private Placement 2Veracyte, Inc. Announces $40 Million Private Placement 3Veracyte, Inc. Announces $40 Million Private Placement 4Early Stage BioTech offers Plenty of Risk; Propanc Health Group Analyst Brief Issued by BrokerBank Securities, Inc. 2Sangamo BioSciences Reports First Quarter 2015 Financial Results 2Sangamo BioSciences Reports First Quarter 2015 Financial Results 3Sangamo BioSciences Reports First Quarter 2015 Financial Results 4Sangamo BioSciences Reports First Quarter 2015 Financial Results 5Sangamo BioSciences Reports First Quarter 2015 Financial Results 6Sangamo BioSciences Reports First Quarter 2015 Financial Results 7Sangamo BioSciences Reports First Quarter 2015 Financial Results 8
... Experience To Antimicrobial Leader.SHREVEPORT, La., March 23 ... in the poultry industry, has joined SteriFx Inc. ... to SteriFx, a leader in developing non-toxic antimicrobial ... defense and other industries. "We,re honored and thrilled ...
... DIEGO, March 23 Nereus Pharmaceuticals, Inc., a ... today announced that it is conducting a randomized ... agent (VDA) NPI-2358 in combination with standard chemotherapy ... (NSCLC). This study follows on positive outcomes ...
... Based on its recent analysis of ... Sullivan presents Nanopoint, Inc. with the 2009 ... Product Innovation of the Year. The company,s ... sophisticated, on-microscope incubator and integrated fluidics system ...
Cached Biology Technology:Dan Blackshear Joins SteriFx Inc. 2Nereus Pharmaceuticals Initiates ADVANCE, a Randomized Phase 2 Clinical Trial of NPI-2358 in Non-Small Cell Lung Cancer 2Nereus Pharmaceuticals Initiates ADVANCE, a Randomized Phase 2 Clinical Trial of NPI-2358 in Non-Small Cell Lung Cancer 3Frost & Sullivan Recognizes Nanopoint for its Pioneering Cell Imaging Solution, the cellTRAY(R) Imaging System CT-2000 2Frost & Sullivan Recognizes Nanopoint for its Pioneering Cell Imaging Solution, the cellTRAY(R) Imaging System CT-2000 3Frost & Sullivan Recognizes Nanopoint for its Pioneering Cell Imaging Solution, the cellTRAY(R) Imaging System CT-2000 4Frost & Sullivan Recognizes Nanopoint for its Pioneering Cell Imaging Solution, the cellTRAY(R) Imaging System CT-2000 5
(Date:3/24/2015)... 24, 2015 Research and Markets ( http://www.researchandmarkets.com/research/kvdtcw/face_and_voice ) ... - Global Strategic Business Report" report to their offering. ... Biometrics in US$ Thousands by the following Segments: Face Biometrics, and ... Canada , Japan , ... Middle East & Africa , and ...
(Date:3/23/2015)... BOSTON, Mass , March 23, 2015 SoundView ... shipment of NXT-ID,s (NASDAQ: NXTD ) Wocket smart wallet. SoundView ... world user feedback on their experience with the Wocket in ... experience at CVS, Whole Foods and other retailers, making both ... Kris Tuttle also says, "If the company meets ...
(Date:3/23/2015)... OXFORD, Conn. , Mar. 23, 2015 NXT-ID, Inc. ... authentication company focused on the growing mobile commerce market, announces ... a new advertising campaign on CNBC television starting March 30 ... ads will commence airing in New York ... Executive Officer said: "We are excited about our new ad ...
Breaking Biology News(10 mins):Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 3Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 4NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3
... appearing in the May issue of The Journal of ... account for when examining dinosaur tracks. The authors studied ... dinosaurs that includes the T. Rex and the tridactyl, ... many different types of erosion on these invaluable impressions. ...
... resulting from farming argue for a shift toward ... energy and more recycling of energy and materials, ... 2007 issue of BioScience. The author, Craig J. ... semiclosed agricultural systems -- which he terms "regenerative" ...
... of the online, open-access journal PLoS ONE, shows that although ... are also large areas of forests regenerating. The return of ... area, with an overall net increase of 4 % during ... of plant endemism (species found only in a particular region) ...
Cached Biology News:How to look at dinosaur tracks 2Madagascan tropical forests return thanks to better management and well-defined ownership 2
Recommended for use as a cell culture substratum at 0.1-0.2 mg/cm 2 or 5-10 μl/cm 2 . Optimal concentration depends on cell type as well as the application or research objectives. Type B...
... The Eclipse is a state-of-the-art separation ... Fractionation (AFFF) technique. The Eclipse integrates easily ... DAWN, miniDAWN Tristar, as well as the ... size and molar mass distributions of the ...
... for determining the absolute count of CD34+ ... peripheral blood samples, and leukapheresis samples. These ... cell count and a total CD45 (leucocyte) ... use in the BD Procount progenitor cell ...
... Protein A and, until recently, no readily available ... purification. Standard methods for IgM purification generally are ... for removing all of the major impurities present ... Pierce has developed an easy-to-use affinity matrix and ...
Biology Products: