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Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,,,Performance


Improve reliability of PCR reactions with Herculase enhanced DNA polymerase

Michael Borns Holly Hogrefe
Stratagene

Stratagene recently released Herculase enhanced DNA polymerase,*, a novel enzyme formulation that provides superior performance in both routine and demanding PCR applications. Previous comparisons showed that Herculase DNA polymerase exhibits greater fidelity than commercial DNA polymerase blends, in addition to producing higher product yields over an extremely broad range of target sizes (0.1 to 48 kb). In this follow-up article, we compared Herculase DNA polymerase to single-enzyme formulations and found it to be more reliable than Taq DNA polymerase in PCR applications; it provided higher product yield, greater sensitivity, and improved synthesis of difficult (long, multiplex, GC-rich) targets. Herculase DNA polymerase was also the superior performer when compared to proofreading PCR enzymes. Moreover, its accuracy was equal to or greater than the accuracy of all proofreading DNA polymerases except Stratagenes Pfu and PfuTurbo DNA polymerases. *,

Herculase enhanced DNA polymerase was developed to meet the need for a single PCR reagent for a broad range of PCR applications.1 Because of its unique Pfu-based composition, Herculase DNA polymerase has distinct properties that distinguish it from Taq-based DNA polymerase blends (formulated as described2). Previous comparisons to commercial DNA polymerase blends showed that Herculase DNA polymerase exhibited higher fidelity, broader target-length capability (0.1 to 48 kb), and superior amplification of extra-long targets (>20 kb).1

Hence, we tested Herculase DNA polymerase in PCR applications that are routinely carried out using single-enzyme formulations. The Herculase formulation was
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