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Unique DNA Polymerase Formulation Excels in a Broad Range of PCR,,,Applications

e study, comparing the fidelity of TaqPlus Precision DNA polymerase to other vendors DNA polymerase blends, indicate that the Herculase formulation exhibits greater accuracy than at least seven additional blends.2

Fig.3

The effects of DMSO on the error rates of Taq, Pfu, and DNA polymerase blends have not been previously described despite DMSOs widespread use as a PCR adjunct. The utility of DMSO in amplifications of extra-long targets prompted us to test its effects on the fidelity of Herculase enhanced DNA polymerase. Preliminary results have shown that DMSO may increase the error rate of the Herculase formulation, albeit slightly. The average error rate of the Herculase formulation was 32% higher in the presence of 3% DMSO, compared to reactions carried out in the absence of DMSO (data not shown). However, the mean error rate of the Herculase formulation in the presence of 3% DMSO is still lower than the mean error rates of commercial Taq-based blends (Table 2, Figure 3). Therefore, using Herculase enhanced DNA polymerase eliminates the need for specialized high-fidelity DNA polymerase blends.

Conclusions

Stratagenes Herculase enhanced DNA polymerase provides accurate and robust amplification over a broad range of target lengths and complexities. The broad utility and superior performance of the Herculase formulation eliminates the need for multiple specialized PCR enzymes and DNA polymerase blends.

Methods

The PCR fidelity assay was carried out as described,1,3 using PfuTurbo DNA polymerase (Stratagene), Herculase enhanced DNA polymerase (Stratagene), TaqPlus Precision PCR system (Stratagene), Taq2000 DNA polymerase (Stratagene), Platinum Taq D
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