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Unique DNA Polymerase Formulation Excels in a Broad Range of PCR,,,Applications

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The fidelity of Herculase enhanced DNA polymerase was measured using the previously described PCR mutation assay.1 The Herculase formulation exhibits an average error rate of 2.8 x 10-6 mutation frequency per base pair per duplication (Table 2). In side-by-side comparisons, the error rate of the Herculase formulation was found to be 2.1-fold higher than the error rate of PfuTurbo DNA polymerase and 1.6-fold and 3.7-fold lower than the error rate of the TaqPlus Precision blend and Taq DNA polymerase, respectively. The Pfu-based composition of the Herculase formulation presumably contributes to its higher fidelity, compared to Stratagenes TaqPlus Precision and TaqPlus Long DNA polymerase blends.

Comparisons to previous data indicate that Herculase enhanced DNA polymerase exhibits an error rate that is comparable (Vent, Deep Vent) or superior [Platinum Pfx (KOD)] to the error rates of certain proofreading single-enzyme preparations (Table 2). Moreover, side-by-side comparisons show that the Herculase formulation exhibits significantly greater accuracy than DNA polymerase blends (Figure 3), including blends formulated specifically for high-fidelity (Platinum Taq DNA polymerase High Fidelity, Advantage HF PCR kit) and long (LA Taq, Expand 20 kbPlus PCR system) PCR applications (Figure 3). The results of a more comprehensiv
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