( 8-kb lambda target was unsuccessful usi...)Unique DNA Polymerase Formulation Excels in a Broad Range of PCR,,,Applications

8-kb lambda target was unsuccessful using the Expand 20 kb^Plus PCR system (Figure 2). In PCRs employing short targets (<1 kb), the Expand 20 kb^Plus PCR system did not produce as much product as the Herculase formulation (Figure 1). Therefore, Herculase enhanced DNA polymerase exhibits superior performance over a full range of amplicon sizes (0.1 kb to 48 kb), and its use eliminates the need for multiple PCR enzymes and specialized long and extra-long PCR blends.

PCR Fidelity Comparisons

Table 2: Error Rates of PCR Enzymes and Blends

Current Assay Results

Published lacI Assay Results

DNA Polymerase

#PCRs

Mean E.R.
(x10^-6) ( Std. dev.)

#PCRs

Mean E.R.
(x10^-6) ( Std. dev.)

PfuTurbo

4

1.3 0.1

14

1.3 0.2^1,3,4

Herculase

4

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Source:


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