( XXXXX ...)Unique DNA Polymerase Formulation Excels in a Broad Range of PCR,,,Applications

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a The results of numerous amplification reactions have been used to rank the PCR performance of Stratagenes PCR enzymes from highest performance

(XXXXX: Greatest yield/successful amplification of >20 kb targets) to lowest performance (X: Product yield and target-length capability (<5 kb) comparable to Taq DNA polymerase). Relative fidelity, determined from error rate measurements, is indicated from most accurate (XXXXX) to least accurate (X: Comparable to Taq, 8 x 10^-6 MF/bp/d^1,3).

Herculase Enhanced DNA Polymerase

The Herculase enzyme formulation has been extensively tested using primer-template systems of varying lengths, complexities, GC content, and DNA template sources. It has been used successfully to amplify genomic targets 0.1 kb to 37 kb in length and lambda DNA targets up to 48 kb in length. The Herculase enhanced DNA polymerase is provided with an optimized PCR reaction buffer and DMSO. As discussed below, adding DMSO can increase product yield and extend target-length capability while having minimal effects on fidelity. For genomic targets greater than 23 kb and lambda targets greater than 30 kb, we recommend adding DMSO to a final concentration of 3% to 7%, depending upon template source, template GC content, and amplicon length. Since the optimal DMSO concentration varies with each primer-template system, providing DMSO as a supplement rather than including it in the PCR reaction buffer ensures that maximal product yields are achieved.

Yield and Target-Length Comparisons

Fig.3

The PCR performance of Herculase enhanced DNA polymerase was tested relative to Platinu
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