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Unique DNA Polymerase Formulation Excels in a Broad Range of PCR,,,Applications

blends. Although DNA polymerase blends can be formulated to favor increased fidelity (higher proportion of proofreading enzyme) or enhanced amplification of longer targets (higher proportion of nonproofreading enzyme), variations in polymerase ratios and buffer composition to achieve increased fidelity generally lead to reduced product yield and poor amplification of long targets. Moreover, DNA polymerase blends and PCR buffers optimized for amplification of long targets generally exhibit poor fidelity and inefficient amplication of short targets.

Stratagenes Herculase enhanced DNA polymerase was developed to provide customers with a single PCR reagent to fulfill all PCR application needs. The Pfu-based Herculase formulation exhibits several unique features, including high fidelity, broad target-length capability, and superior amplification of extra-long targets which make it ideal for a full range of routine and demanding PCR applications (Table 1).

Comparisons to Taq(= x)a

Herculase Enhanced DNA Polymerase

PfuTurbo DNA Polymerase

TaqPlus Precision PCR System

TaqPlus Long PCR System

Performance
(yield & length)

XXXXX

XX

XXX

XXXX

Fidelity

XXXX


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