Navigation Links
Unique DNA Polymerase Formulation Excels in a Broad Range of PCR,,,Applications


Herculase enhanced DNA polymerase delivers high fidelity and great performance

Michael Borns Holly Hogrefe
Stratagene

Stratagene has recently released Herculaseenhanced DNA polymerase,*, a novel enzyme formulation that provides superior performance in both routine and demanding PCR applications. Comparisons demonstrate that Herculase enhanced DNA polymerase is even more robust than commercial DNA polymerase blends, producing superior product yields in amplifications of both short (<1 kb) and extra-long (>20 kb) genomic targets. In addition to high product yields over a broad range of target sizes, the Herculase enhanced DNA polymerase exhibits greater fidelity than Taq DNA polymerase, several proofreading DNA polymerases, and all commercial DNA polymerase blends tested.

Over the past few years, a number of DNA polymerases and polymerase blends have been developed to fulfill a variety of PCR application requirements. Taq DNA polymerase is generally used in routine amplifications of short targets (<2 kb), while Stratagenes Pfu and PfuTurbo DNA polymerases*, are the best choices for cloning applications requiring the highest fidelity PCR enzyme available. DNA polymerase blends are used to successfully amplify long targets (>5 kb), and additionally may provide higher product yields, greater sensitivity, increased accuracy, and faster cycling times than are achieved with Taq DNA polymerase alone. Polymerase vendors may offer as many as 2 to 5 different DNA polymerase blends, developed to satisfy particular fidelity, target-length, and DNA template (%GC content, cDNA, genomic DNA) requirements.

Until now, no PCR enzyme possesses all of the features necessary to fulfill a broad range of PCR application needs. Single-enzyme formulations exhibit limited target-length capability compared to DNA polymerase
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
3. A Unique Dimension in Biological Purity
4. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
5. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
6. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
7. Pfu DNA Polymerase as a Hot Start Alternative
8. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
9. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
10. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
11. High-Fidelity PCR with a Novel Polymerase Mixture
Post Your Comments:
(Date:7/28/2015)... -- Research and Markets ( ... "Analysis of the Global Flow Cytometry Market ... Globally, flow cytometry remains a popular tool for ... stem cells. But the market is witnessing active ... from underdeveloped nations to quantitate CD4 cell markers ...
(Date:7/28/2015)... ... ... VetStem Biopharma will be offering weekly private guided tours of our new ... The tour and course will be given every Wednesday at 6pm to local interested ... members are welcome to attend with their veterinarian as well. Participants will be able ...
(Date:7/28/2015)... 2015 Tauriga Sciences, Inc. (OTCQB:  TAUG) or ... today announced that its stockholders approved an increase in ... the Company from 1,000,000,000 to 2,500,000,000 at its Special ... the Law Offices of Nixon Peabody LLP in Midtown ... were 480,655,929 shares of common stock represented either by ...
(Date:7/28/2015)... 2015  Moerae Matrix Inc. announced today the ... with MMI-0100, a first-in-class inhibitor of MAPKAP kinase ... disorders characterized by inflammation and fibrosis.  The study, ... , is a double-blind, two-way cross-over design lipopolysaccharide ... tolerability of MMI-0100 when given via inhalation to ...
Breaking Biology Technology:Analysis of the Global Flow Cytometry Market 2012-2018 with a 2014 Base Year and a 2015-2018 Forecast Period 2VetStem Biopharma weekly veterinary continuing education classes and GMP manufacturing facility tour 2Tauriga Sciences, Inc. Stockholders Approve Increase In Authorized Common Stock 2Tauriga Sciences, Inc. Stockholders Approve Increase In Authorized Common Stock 3Moerae Initiates Lipopolysaccharide (LPS) Challenge Study of Inhaled MMI-0100 for Pulmonary Disease Following Encouraging Safety and Tolerability Observed in Single Ascending Dose Phase 1 Study 2
... Gen-Probe Incorporated,(Nasdaq: GPRO ) announced today that the ... Investment Conference on Tuesday, September 16, 2008 at,approximately 8 ... live and may be accessed through a link on ... The webcast will be,available for 30 days following the ...
... China, Sept. 10 /Xinhua-PRNewswire-FirstCall/ -- China Yingxia,International, Inc. ... a leading provider in the nutraceutical industry engaged ... products,dietary supplements, and personal care products in the ... of Mr. Fanke Kong as its new,Vice President ...
... number of US clinics, CHICAGO, IL, Sept. ... first commercially available bionic hand, today revealed details ... Assembly of the American,Orthotic and Prosthetic Association (AOPA). ... RACE Team and Partner Program are designed to ...
Cached Biology Technology:Gen-Probe to Webcast Presentation at the Bank of America 38th Annual Investment Conference 2China Yingxia International, Inc. Appoints New Vice President of Finance 2China Yingxia International, Inc. Appoints New Vice President of Finance 3Touch Bionics unveils innovative partner and reimbursement programs at AOPA 2Touch Bionics unveils innovative partner and reimbursement programs at AOPA 3
(Date:7/2/2015)... 25, 2015 Research ... of the "Natural Language Processing Market by ... OCR, Pattern & Image Recognition) - Worldwide Forecast ... The key vendors occupying the market are ... Microsoft Corporation, NetBase Solutions, SAS Institute Inc., Verint ...
(Date:6/29/2015)... -- Research and Markets( http://www.researchandmarkets.com/research/zj7h4f/latin_america ) ... Biomedical Sensors Market - Growth, Trends & Forecasts (2014-2020)" ... America Biomedical Sensors market is estimated at $0.65 billion ... period 2014-2020 The near future will bring ... of each individual. These sensors can be set to ...
(Date:6/24/2015)... , June 24, 2015 The biologics ... The major drivers for the market include growth of ... of new drug launches. Over the years, the number ... Between 2001 and 2010, the FDA approved 225 drug ... to WIPO, Europe accounted for ...
Breaking Biology News(10 mins):Natural Language Processing Market by Type (Rule-Based, Statistical, and Hybrid), Technologies (Recognition, IVR, OCR, Pattern & Image Recognition) - Worldwide Forecast to 2020 2Latin America Biomedical Sensors Market Report 2015-2020 2Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 2Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 3Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 4
... time, researchers have confirmed an association between a common ... brain MRI, according to a new study published online ... raises the possibility that a toxic component of the ... administration. Brain MRI exams are often performed ...
... reduced when they are exposed in early infancy to a ... reason why. Exposure of mice to dust from houses ... reshape the community of microbes that live in the mouse ... also diminish immune system reactivity to common allergens, according to ...
... The groundbreaking Intergovernmental Platform on Biodiversity and Ecosystem ... of assessments on pollination and food production, land ... with the tools to tackle pressing environmental challenges. ... governments, scientific organizations, civil society and the private ...
Cached Biology News:Contrast agent linked with brain abnormalities on MRI 2Research shows how household dogs protect against asthma and infection 2Research shows how household dogs protect against asthma and infection 3Pollination, land degradation: Top priorities for assessment by new UN intergovernmental body 2Pollination, land degradation: Top priorities for assessment by new UN intergovernmental body 3