Herculase enhanced DNA polymerase delivers high fidelity and great performance
Michael Borns Holly Hogrefe
Stratagene has recently released Herculaseenhanced DNA polymerase,*, a novel enzyme formulation that provides superior performance in both routine and demanding PCR applications. Comparisons demonstrate that Herculase enhanced DNA polymerase is even more robust than commercial DNA polymerase blends, producing superior product yields in amplifications of both short (<1 kb) and extra-long (>20 kb) genomic targets. In addition to high product yields over a broad range of target sizes, the Herculase enhanced DNA polymerase exhibits greater fidelity than Taq DNA polymerase, several proofreading DNA polymerases, and all commercial DNA polymerase blends tested.
Over the past few years, a number of DNA polymerases and polymerase blends have been developed to fulfill a variety of PCR application requirements. Taq DNA polymerase is generally used in routine amplifications of short targets (<2 kb), while Stratagenes Pfu and PfuTurbo DNA polymerases*, are the best choices for cloning applications requiring the highest fidelity PCR enzyme available. DNA polymerase blends are used to successfully amplify long targets (>5 kb), and additionally may provide higher product yields, greater sensitivity, increased accuracy, and faster cycling times than are achieved with Taq DNA polymerase alone. Polymerase vendors may offer as many as 2 to 5 different DNA polymerase blends, developed to satisfy particular fidelity, target-length, and DNA template (%GC content, cDNA, genomic DNA) requirements.
Until now, no PCR enzyme possesses all of the features necessary to fulfill a
broad range of PCR application needs. Single-enzyme formulations exhibit limited
target-length capability compared to DNA polymerase