( leic acids. ...)Ultra-Fast Purification of Small Nucleic Acids

With this system, you can rapidly purify nucleic acids smaller than 40 bases by terminating electrophoresis at the time of dye elution, visible through the instrument's front window. All molecules running through the gel are deposited in the specialized lower collection chamber and can be withdrawn at any point throughout the run. Figure 2 demonstrates the successful isolation of Ambion's Decade Markers (RNA molecules between 10150 nt) using the flashPAGE Fractionator System. In this experiment, two samples were removed, separated by the flashPAGE A40 dye molecule. Following the PAGE run, the flashPAGE Reaction Clean-up Kit is available separately for rapid glass fiber filter (GFF) concentration of nucleic acids and removal of small molecular contaminants.

Figure 2. flashPAGE Fractionation. Decade Markers (Ambion) in a background of 10 g mouse brain total RNA were loaded onto a pre-cast flashPAGE Gel Cartridge and electrophoresed with the flashPAGE Fractionator. Two successive fractions were collected. For the first fraction, the lower buffer was collected and precipitated when the dye indicator band had reached the lower end of the gel cartridge. After adding fresh lower buffer to the apparatus, the sample was electrophoresed for 10 more minutes. The second lower buffer fraction was then collected and precipitated.

High Performance

Each flashPAGE Pre-cast Gel can accommodate up to 100 g total nucleic acid per column (approximately 10 ng
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