Comparison of flashPAGE and Traditional PAGE Protocols.
The flashPAGE System is named to reflect the substantial time and labor savings over traditional PAGE purification of nucleic acids. With it, you will easily and efficiently purify small nucleic acid molecules in run times averaging only 12 minutes.
flashPAGE purification is based on passing up to 10 g nucleic acids through a proprietary denaturing gel matrix, provided as pre-cast gel cartridges, into the lower buffer collection chamber. The lower buffer chamber has been designed for retention of eluted material to facilitate nucleic acid purification. In just a few simple steps, you'll eliminate hours to days of labor (see Figure 1).
Consistent Elution of Nucleic Acids
The flashPAGE Gels provide consistent separation of molecules, providing predictable separation of small, single-stranded nucleic acids within seconds. Using this system, you can purify RNA or DNA molecules of various sizes using time-based elution and standard controls.
The flashPAGE A40 Dye Marker, included in
the flashPAGE Buffer Kit, elutes at the same molecular weight
as a single-stranded RNA molecule of 40 bases. Under normal
running conditions, this dye molecule elutes after
approximately 12 minutes and can be used as a standard
reference point for elution of nuc