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ix plasmid DNA (25 g/ml final concentration, in bidistilled H2O).
  • Transfer 800 l cell suspension into electroporation cuvettes (4 mm gap width). The cell suspension must be free of air bubbles.
  • Electroporation:

    Mode Eukaryotes Voltage (V) 620 V Time constant (T) 60 s No. of pulses (n) 1
  • After the pulse, allow the cell suspension to stand in the cuvette for 15 minutes at room temperature.
  • Carefully transfer the cell suspension from the cuvette to 3 ml RPMI / 10% FCS, and cultivate them in a 55 mm culture dish.
    • Detection methods for transfection:
    The expression of the plasmid pEGFP-N1 can be detected clearly after 24-48 hours with the aid of FACS analysis or under a fluorescence microscope. Result: Survival rate: 50% Transfection rate: 24% based on the initial number of cells used for the experiment Results were measured 48 hours after transfection.


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