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-20 l/ml final concentration, in bidistilled H2O) The cell suspension must be free of air bubbles.
  • Electroporation:

    Mode Eukaryotes Voltage (V) 600 V Time constant (T) 60 s No. of pulses (n) 1
  • After the pulse, allow the cell suspension to stand in the cuvette for 5-10 minutes at room temperature.
  • Carefully transfer the cell suspension from the cuvette into 3-5 ml IMDM / 10% FCS, and cultivate them in a 55 mm culture dish.
  • Detection methods for transfection:
    The expression of the plasmid pEGFP-C1 can be detected clearly after 24-48 hours with the aid of FACS analysis or under a fluorescence microscope. Result: Survival rate: 62% Transfection rate: 23% based on the number of surviving cells. Results were measured 48 hours after transfection.


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