Navigation Links
Trouble-shooting: Smeared bands

Nucleotide concentration is too high or unbalanced

The standard concentration is 20-200 M of each nucleotide

Suggestions:

  1. Check the concentration of stock solutions of all nucleotides
  2. Double check the final concentrations of all nucleotides
Back to Top DNA contamination / carry-over Suggestions:
  1. Test for carryover by performing PCR without adding target DNA.
  2. Avoid carryover (see below)
To prevent carryover, good lab practices should be used such as:

  • Physically isolating PCR preps and products
  • Autoclaving solutions, tips, and tubes
  • Aliquoting reagents to minimize repeated sampling (no more than 20 reactions per aliquote)
  • Eliminating aerosols by using positive displacement pipettes
  • Premixing reagents
  • Adding DNA to reaction last
  • Choosing (+) and (-) controls carefully
  • Soaking gel box and combs in 1M HCl to depurinate DNA
  • Using new razor blades to exise bands
  • Using new razor blades to exise bands
  • Covering UV box with fresh plastic wrap
  • Always using oil overlay

  • '"/>


Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Trouble-shooting: Low yield
2. Trouble-shooting: Non-specific bands
3. Trouble-shooting: Misincorporation or low fidelity
4. Trouble-shooting: No product
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Trouble shooting Smeared bands

(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
(Date:12/22/2014)... 22, 2014 Fertility Associates of Memphis ... Bank USA , a network of more than 20 ... partnership, patients have fast access to a national pool ... IVF . Fertility Associates of Memphis is the only ... treatment option. , Dr. William Kutteh , director ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one ... of Nick Maroulis, Pharm.D. to the newly created position ... In this position, Dr. Maroulis will continue to manage ... of our multi-site pharmacies as the company expands. Dr. ... that time he has served in many different capacities ...
(Date:12/19/2014)... Dec. 19, 2014 Bina Technologies, Inc. (Bina, ... that they have been acquired by Roche (SIX: RO, ... privately held company that provides a big data platform ... (NGS) data for the academic and translational research markets. ... and will continue to focus on development of their ...
Breaking Biology Technology:WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3
... 15, 2011 diaDexus, Inc. (OTC Bulletin Board: ... commercialization of patent-protected in vitro diagnostic products addressing ... the second quarter of 2011. Total revenues for ... 45% growth over total revenues of $2.6 million for the ...
... 3-V Biosciences, Inc. announced today the appointment of ... (Logo:   http://photos.prnewswire.com/prnh/20100513/LA04336LOGO ) "We are ... of the 3-V senior management team," said Merdad V. ... experience in drug discovery and development, as well as ...
... CA (July 1, 2011) After Fukushima, it is now ... - from cradle to grave. If nuclear waste management is ... countries will reject nuclear power as an energy choice, according ... the Atomic Scientists , published by SAGE. According to ...
Cached Biology Technology:diaDexus, Inc. Reports 45% Year-Over-Year Revenue Growth for 2011 Second Quarter 2diaDexus, Inc. Reports 45% Year-Over-Year Revenue Growth for 2011 Second Quarter 3diaDexus, Inc. Reports 45% Year-Over-Year Revenue Growth for 2011 Second Quarter 4diaDexus, Inc. Reports 45% Year-Over-Year Revenue Growth for 2011 Second Quarter 53-V Biosciences Appoints George W. Kemble, PhD, as Chief Scientific Officer 2Nuclear waste requires cradle-to-grave strategy 2Nuclear waste requires cradle-to-grave strategy 3
(Date:12/19/2014)... , Dec. 18, 2014  23andMe, Inc., the ... a study that pinpoints fine-scale differences in genetic ancestry ... . Since immigrants first arrived more ... States has served as a meeting place ... American history and the ongoing mixing of peoples with ...
(Date:12/17/2014)... Dec. 16, 2014 Valencell, a leader in ... biometric technology to industry leaders such as Intel, Jabra, ... validated, biometric wearable products. These products will be showcased ... Las Vegas . "Our ... flexible and robust – with the clinical data to ...
(Date:12/11/2014)... , Dec. 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced ... India 2015-2019" report to their offering. ... market is the adoption of multimodal biometric systems. ... of an individual for verification and identification purposes. ...
Breaking Biology News(10 mins):23andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... Va. , June 27, 2013 IriTech, Inc., ... member of the Texas Instruments Design Network, today released ... further empower its prevailing portable IriShield TM –USB ... Windows/Linux-based PCs but also Android-based mobile devices. The two ...
... say is the first public health study of the ... a UC Davis study analyzed emergency department records from ... in the summer of 2005. Physicians and scientists from ... Health found no increase in specific diagnoses that are ...
... in Munich have developed a new method for visualizing ... research team led by Bert Nickel has, for the ... in organic thin-film solar cells using laser light for ... in the scientific journal " Advanced Materials ". "We ...
Cached Biology News:IriTech launches iris-recognition-based solutions for workforce and identity management 2IriTech launches iris-recognition-based solutions for workforce and identity management 3Aerial mosquito spraying study finds no immediate public health risks 2Aerial mosquito spraying study finds no immediate public health risks 3Organic electronics: Imaging defects in solar cells 2
... enzyme that catalyzes the hydrolysis of,starch ... and dextrins. The,level of a-amylase in ... is of,clinical significance in the diagnosis ... plant and microbial a-amylases are,important enzymes ...
Rat monoclonal [20d5] to NKG2A + C+ E ( Abpromise for all tested applications). Antigen: Tissue / cell preparation (Mouse): CHO transfected cells expressing the B6 allele of NKG2A. Entrez Gen...
... The Thermo Labsystems Wellmix is a ... and time settings. The shaking speed is ... shaking frequency, high speed and a small ... assays. The shaking time is quick and ...
... Fluoroskan Ascent FL ... fluorometric and luminometric measurement ... long list of advanced ... and Luminoskan Ascent. Fluoroskan ...
Biology Products: