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Trouble-shooting: No product

Here are some troubleshooting hints that we have gathered if your PCR yields no product: Non-optimal Mg++ concentration The amount of template in the reaction is not optimal An enzyme inhibitor is present in the reaction Primer annealing temperature is too high or too low Primers are degraded or not optimal Incomplete template denaturation Machine based error Mispriming caused by secondary structure of template, snapback, or excessive homology at 3' ends of primers NaCl concentration above 50 mM KCl concentration above 50 mM Non-optimal Mg++ concentration

Suggestion: Titrate magnesium concentration using our PCR Optimization K
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