Navigation Links
Trouble-shooting: Low yield

ability and work equally well in PCR, sequencing, and labeling applications. Lithium salts are more soluble in ethanol than sodium salts. Thus, removal of lithium salts by ethanol precipitation is more efficient than removal of sodium salts. Using lithium salt nucleotide preparations reduce salt-induced artifacts and increase the legibility of sequencing gels. Back to Top Nucleotide concentration is too high or unbalanced

The standard concentration is 20-200 M of each nucleotide

Suggestions:

  1. Check the concentration of stock solutions of all nucleotides
  2. Double check the final concentrations of all nucleotides
Back to Top Primer concentration is too low

Suggestion:

Adjust the primer concentration (0.1 - 1.0 M of each primer is optimal)

Back to Top

Machine based error Suggestions:
  • Calibrate the heating block and confirm actual block temperature
  • Run a diagnostic program for the particular machine - contact the machine manufacturer for specifics
Back to Top
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Trouble-shooting: Non-specific bands
2. Trouble-shooting: Misincorporation or low fidelity
3. Trouble-shooting: No product
4. Trouble-shooting: Smeared bands
5. Food science: increasing crop yields
6. A technique for increasing yields in bioreactors and disposable cell-culture systems
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Trouble shooting Low yield

(Date:8/28/2014)... DIEGO, California, USA (PRWEB) August 28, 2014 ... the Department of Physics and Optical Science and Director ... the University of North Carolina at Charlotte, and co-founder ... been elected to serve as the 2015 Vice President ... , SPIE 2014 President Philip Stahl announced recent election ...
(Date:8/28/2014)... in spintronics, an electronic which is not only ... on their spin and the spin-related magnetism. Spin-charge ... signals and vice versa. Recently, the research group ... Physics at Johannes Gutenberg University Mainz in collaboration ... has for the first time realised a new, ...
(Date:8/28/2014)... 28, 2014 Sterlitech is proud to ... to its stable of products . These ... cut-offs and include membrane filters with surface charges. ... customer demand for our membrane process testing equipment, and ... flat sheet membranes,” explains Sterlitech President Mark Spatz. ...
(Date:8/28/2014)... Earlier this month, a report was created by ... Air Force on the performance of vehicles using an ... of fuel made from mustard seeds, offered great potential ... Eco friendly future. In fact, every branch of the ... of foreign oil over the next few years. They ...
Breaking Biology Technology:Glenn Boreman, UNC Professor and Plasmonics Co-Founder, Elected to SPIE Presidential Chain 2A new, tunable device for spintronics 2A new, tunable device for spintronics 3Sterlitech Corporation Enhances Its Selection of Flat Sheet Membrane Filters 2Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 2Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 3Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 4Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 5
... MSC Care Management, the industry,s leading provider of ... workers, compensation claimants nationwide, announced today that it ... also focused on the workers, compensation industry, to ... management solution, known as S3. Integrated Healthcare Services, ...
... BETHESDA, Md., Oct. 4, 2011 Northwest Biotherapeutics ... will be presenting at the second annual Cancer ... 6, at the New York Academy of Medicine ... key opinion leaders, industry executives, and other stakeholders ...
... Oct. 4, 2011 Kylin Therapeutics, Inc., a ... has received a notice of allowance from United ... second U.S. patent application. This newly allowed patent ... as expanded areas on Kylin,s proprietary nanoparticle technology ...
Cached Biology Technology:MSC to Offer Surgical Implant Cost Management Solution through Acquisition of Integrated Healthcare Services 2NORTHWEST BIO Will Present at the Second Annual "Cancer Immunotherapy: A Long Awaited Reality Conference" at the New York Academy of Medicine, October 6, 2011 2NORTHWEST BIO Will Present at the Second Annual "Cancer Immunotherapy: A Long Awaited Reality Conference" at the New York Academy of Medicine, October 6, 2011 3NORTHWEST BIO Will Present at the Second Annual "Cancer Immunotherapy: A Long Awaited Reality Conference" at the New York Academy of Medicine, October 6, 2011 4Kylin Therapeutics Receives Notice of Allowance for Second U.S. Patent Covering Delivery of Therapeutic RNA and pRNAi Technology 2
(Date:8/28/2014)... 2014A new method for measuring and imaging how quickly ... researchers better understand how drug abuse affects the brain, ... engineering, and lead to better treatment options for recovering ... of researchers from Stony Brook University in New York, ... published today in The Optical Society,s (OSA) open-access journal ...
(Date:8/28/2014)... 25 million kilometres of new roads will be built ... into Earth,s last wildernesses, where they bring an influx ... Now, an ambitious study has created a ,global roadmap, ... to balance the competing demands of development and environmental ... layer that estimates that natural importance of ecosystems and ...
(Date:8/28/2014)... pose a significant health risk to people with asthma according ... Allergy and Clinical Immunology . , By critically reviewing the ... has found that the presence of several types of mould ... as increasing the likelihood of developing the condition. , The ... of Exeter Medical School and is the first time all ...
Breaking Biology News(10 mins):This is your brain's blood vessels on drugs 2This is your brain's blood vessels on drugs 3Study shows where on the planet new roads should and should not go 2Study shows where on the planet new roads should and should not go 3Indoor mold poses health risk to asthma sufferers 2
... might not know what it,s called, but if you,ve ... it is likely you have received Sellick,s maneuver. That,s ... prevent regurgitation and spilling of stomach contents into the ... Such regurgitation could result in serious lung damage and ...
... researchers from the United States, Korea, and France has sequenced ... E. coli bacteria, one used to study evolution ... practical applications. The findings will help guide future research and ... classical research that is the foundation of our understanding of ...
... New research released today is affirming a long-held maxim: you ... what you eat has a profound influence on the brain. ... obesity epidemic in the United States and other developed countries. ... and weight gain, and were reported at Neuroscience 2009, the ...
Cached Biology News:UF scientists discover new explanation for controversial old patient-care technique 2UF scientists discover new explanation for controversial old patient-care technique 3Genomes of 2 popular research strains of E. coli sequenced 2Genomes of 2 popular research strains of E. coli sequenced 3Think what you eat: Studies point to cellular factors linking diet and behavior 2