Navigation Links
Trouble-shooting: Low yield

href="#Option13">Evaporation Primer annealing temperature is too high

Primer annealing temperature is typically 50-60C (may be higher or lower based on primer sequence and buffer components).

Suggestion:

Determine Tm/annealing temperature based on one of the following equations:

If primers are 20-35 bases If primers are 14 - 70 bases Tp = 22 + 1.46(Ln)
Ln = 2(# G or C) + (# A or T)
TP = Effective annealing
temperature 2 - 5 Tm = 81.5 + 16.6 (log10 [J+]) + 0.41
(% G + C) - (600/l) - 0.063
(% Formamide) + 3 to 12
[J+] = concentration of Monovalent cations
l = length of oligo Back to Top Template not clean or degraded

For example, protease contamination can degrade the polymerase.

Suggestions:

  1. Try "Hot start" technique
  2. Purify the DNA template to the highest degree possible, including proteinase K digest
Back to Top An<
'"/>


Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Trouble-shooting: Non-specific bands
2. Trouble-shooting: Misincorporation or low fidelity
3. Trouble-shooting: No product
4. Trouble-shooting: Smeared bands
5. Food science: increasing crop yields
6. A technique for increasing yields in bioreactors and disposable cell-culture systems
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Trouble shooting Low yield

(Date:6/29/2015)... -- Neuralstem, Inc. (NYSE MKT: CUR), a biopharmaceutical company using ... cell therapy treatments for central nervous system diseases, announced ... the NASDAQ Capital Market under the symbol "CUR." The ... NYSE MKT until the market close on July 10, ... to commence on July 13, 2015. ...
(Date:6/29/2015)... Elsevier , a world-leading ... services, today announced the highlights of its journal Impact ... Citation Reports® (JCR) published by Thomson Reuters, Elsevier saw ... to 2014, ahead of the aggregate across other journals. ... 62 subject categories, up from 61 in 2013. ...
(Date:6/26/2015)... , June 26, 2015  A San Francisco ... products made with synthetic rhino horn: beer in ... Vietnam . In response to the emerging ... manufacture, marketing and sale of synthetic rhino horn.  ... and Save the Rhino International (SRI) released ...
(Date:6/26/2015)... DALLAS , June 26, 2015 ... Animal Fat), Application (Hydraulic Fluids, Metalworking fluids, Chainsaw oils, ... Automobile) & by Region - Global trends and Forecasts ... expected to reach $2,972.13 Million by 2020 at a ... Browse 160 market data Tables and   66 ...
Breaking Biology Technology:Neuralstem, Inc. Announces Approval for Listing on the NASDAQ Capital Market 2Neuralstem, Inc. Announces Approval for Listing on the NASDAQ Capital Market 3Elsevier Announces 2014 Citation Impact Highlights 2Elsevier Announces 2014 Citation Impact Highlights 3Rhino Experts Oppose Synthetic Rhino Horn as Solution to Poaching Crisis 2Biolubricants Market Worth $2,972.13 Million by 2020 2Biolubricants Market Worth $2,972.13 Million by 2020 3
... 5-Year Survival Data for Responding Patients, BERKELEY ... Board: GNTA) announced that the Company has,submitted an ... for its,New Drug Application (NDA) for Genasense(R) (oblimersen ... refractory chronic,lymphocytic leukemia (CLL). The submission is based ...
... CHICAGO, June 5 "AHIMA,s Board of Directors ... strengthen the effort,to address health IT from a strategic ... executives. In addressing the need to,realize real benefits from ... AHIMA will continue working to support the NAHIT,mission and ...
... June 5 BioTrends Research Group, Inc. is,pleased ... II. The report is based on the results ... in the UK, France, Germany, Italy, and Spain.,The ... hyperphosphatemia,and secondary hyperparathyroidism., In the ESA market, ...
Cached Biology Technology:Genta Submits NDA Amendment to FDA for Genasense as Treatment of Chronic Lymphocytic Leukemia 2Genta Submits NDA Amendment to FDA for Genasense as Treatment of Chronic Lymphocytic Leukemia 3Genta Submits NDA Amendment to FDA for Genasense as Treatment of Chronic Lymphocytic Leukemia 4New Study by BioTrends Highlights Practice Management Shifts Among European Nephrologists 2
(Date:6/25/2015)... June 25, 2015  Imagine a tool specifically designed ... healthier, happier life. That,s exactly what USANA,s True ... The cutting-edge, portable health program provides a personalized approached ... help improve your lifestyle and nutrition. ... gala Wednesday night, USANA,s THA was honored with the ...
(Date:6/24/2015)... , June 22, 2015 ... announced the addition of the "Huawei Ascend ... report to their offering. Huawei,s ... to the opposite direction than Apple,s and Samsung,s ... fingerprint sensor developed by Fingerprint Cards, a main ...
(Date:6/23/2015)... , June 23, 2015  Crossmatch™, ... and authentication solutions, today announced enhanced functionality of ... authentication solution.  The enhancements build on the ... the DigitalPersona Altus platform and provide expanded ... In today,s environment of increasing ...
Breaking Biology News(10 mins):@USANAinc's Personalized Nutrition Program Wins Industry Innovation Award 2Huawei Ascend Mate 7 Fingerprint Sensor - Reverse Costing Analysis 2Crossmatch Releases Enhanced DigitalPersona Altus Advanced Authentication Functionality 2
... Lee Lovering, Ph.D., School of Biosciences, University of ... Award for his seminal work on the structural ... and modify cell walls in bacteria. Natalie Strynadka, ... Lovering,s work: "his spectacular abilities in structural biology ...
... 2012) Aeras and the Infectious Disease Research Institute ... trial of IDRI,s novel tuberculosis vaccine candidate, ID93 + ... safety, tolerability and immunogenicity of the vaccine candidate in ... by Johnson County Clin-Trials in Lenexa, Kansas, in close ...
... risks posed to people who work with tiny fibres used ... Research into the health risks posed by nanofibres ... has pinpointed the lengths at which these fibres are ... from a range of materials including carbon, are about 1,000 ...
Cached Biology News:The American Society for Microbiology honors Andrew Lovering 2As TB grows more difficult to control, vaccine candidate to prevent disease enters clinical testing 2
Mol wt: monoisotopic mol wt1,045.5423 Da by calculation...
Bis-Acrylamide (bis) is a monomer used as a crosslinker with acrylamide to form polyacrylamide electrophoretic gels. This 2% bis solution is supplied as 500 ml....
Anti-human VEGFR-2/KDR (Protein-A Purified) Polyclonal Antibody...
Mouse monoclonal antibody to Lats2....
Biology Products: