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TripleMaster and Perfect gDNA Blood Mini Kit Team Up,,, to Amplify Long gDNA Templates

ectrophoresis experiment. Eppendorf samples average about 70 kb. It appears that blood and final sample storage conditions do not affect the overall size of the genomic DNA. The gDNA isolated using the Qiagen product averages between 23 and 48.5 kb. The presence of larger amounts of higher molecular weight gDNA in the Eppendorf samples provide a significant advantage for long-range PCR efforts. In the second experiment, using the Eppendorf TripleMaster PCR System, DNA is easily amplified in both the 22 kb and 24 kb PCR reactions. In fact, lanes with the Eppendorf-isolated gDNA are overloaded with product. The TripleMaster PCR System is able to ensure some amplification of the 22 kb fragment even with the lower molecular weight DNA isolated using the Qiagen product, but the 24 kb reaction generates no amplification product.

Fig. 1: Field inversion gel electrophoresis

Lane 1: Low Range PFG Marker Lane 2: Lambda Hind III Marker Lane 3: Eppendorf 4C, 1 day Lane 4: Qiagen 4C, 1 day Lane 5: Eppendorf 4C, 2 months Lane 6: Qiagen 4C, 2 months Lane 7: Eppendorf-20C, 1 day Lane 8: Qiagen 20C, 1 day
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