Navigation Links
TripleMaster and Perfect gDNA Blood Mini Kit Team Up,,, to Amplify Long gDNA Templates

ectrophoresis experiment. Eppendorf samples average about 70 kb. It appears that blood and final sample storage conditions do not affect the overall size of the genomic DNA. The gDNA isolated using the Qiagen product averages between 23 and 48.5 kb. The presence of larger amounts of higher molecular weight gDNA in the Eppendorf samples provide a significant advantage for long-range PCR efforts. In the second experiment, using the Eppendorf TripleMaster PCR System, DNA is easily amplified in both the 22 kb and 24 kb PCR reactions. In fact, lanes with the Eppendorf-isolated gDNA are overloaded with product. The TripleMaster PCR System is able to ensure some amplification of the 22 kb fragment even with the lower molecular weight DNA isolated using the Qiagen product, but the 24 kb reaction generates no amplification product.

Fig. 1: Field inversion gel electrophoresis

Lane 1: Low Range PFG Marker Lane 2: Lambda Hind III Marker Lane 3: Eppendorf 4C, 1 day Lane 4: Qiagen 4C, 1 day Lane 5: Eppendorf 4C, 2 months Lane 6: Qiagen 4C, 2 months Lane 7: Eppendorf-20C, 1 day Lane 8: Qiagen 20C, 1 day
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. TripleMaster PCR System
2. TripleMaster PCR System
3. The TripleMaster PCR System for three different amplifications
4. TripleMaster PCR System: Direct Comparison with Competitors
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
8. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
9. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
10. Eppendorf Perfectprep Gel Cleanup Kit
11. Eppendorf Perfectprep Gel Cleanup Kit
Post Your Comments:
(Date:1/22/2015)... Jan. 22, 2015  Transwestern | RBJ today announces the firm ... space for Shire a leading biopharmaceutical company, at Two Ledgemont ... RBJ,s Robert Richards , president, and Brian Cohen ... the entire five-floor building at 95 Hayden Ave. ...
(Date:1/22/2015)... BOSTON , Jan. 22, 2015  Transwestern | RBJ today ... feet of office space for Shire a leading biopharmaceutical company, ... . Transwestern | RBJ,s Robert Richards , president, and ... the lease for the entire five-floor building at 95 Hayden ...
(Date:1/22/2015)... The laboratory information management systems market ... number of technological advancements due to factors such as ... to integrate healthcare systems, and increasing government support for ... integration. Key players in the market focus on technological ...
(Date:1/22/2015)... / Rootstown, OH (PRWEB) January 22, 2015 ... platform, announced today that it has received AOAC-PTM Certifications for ... O1O3, O111, O121, and O145; collectively referred to as STEC ... O157, at 1 colony forming unit (cfu) per 325 g ...
Breaking Biology Technology:Transwestern | RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 3Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 4Crystal Diagnostics Awarded AOAC-PTM Accreditation for the Rapid Detection of “Big 6” E.coli Food Pathogens 2
... ... bar coding, scan and software tracking of each part and batch, including all leak ... ... -- Automotive, medical device, appliance and other manufacturers seeking cost-effective ways to collect and ...
... BOZEN, Sud-Tirol, Italy , April 26, 2010 ... the International Society of,Oncology Pharmacy Practitioners (ISOPP), B|Braun Melsungen, ... host ISOPP delegates at its bi-annual congress,to witness live ... the only robot in the world proven to compound ...
... ... and Increase Yields , ... Oceanside, CA (PRWEB) April 26, 2010 -- Kinovate Life Sciences, Inc., a California ... oligonucleotide synthesis, will be launching its next generation high loading polymeric solid support product ...
Cached Biology Technology:InterTech Development Company Component Tracker System Announced 2Health Robotics to Showcase CytoCare at ISOPP (May 2010) and Charles University' General Faculty Hospital of Prague 2Health Robotics to Showcase CytoCare at ISOPP (May 2010) and Charles University' General Faculty Hospital of Prague 3Kinovate Life Sciences, Inc. and Nitto Denko Corporation Announce Launch of NittoPhaseHL – High Loaded Solid Support for Oligonucleotide Synthesis 2Kinovate Life Sciences, Inc. and Nitto Denko Corporation Announce Launch of NittoPhaseHL – High Loaded Solid Support for Oligonucleotide Synthesis 3Kinovate Life Sciences, Inc. and Nitto Denko Corporation Announce Launch of NittoPhaseHL – High Loaded Solid Support for Oligonucleotide Synthesis 4
(Date:1/22/2015)... Wis. , Jan. 15, 2015  BellBrook Labs, ... drug discovery, announced the launch of a TR-FRET ... the company,s Transcreener UDP Assay, a high throughput ... The new assay will allow for sensitive detection ...
(Date:1/22/2015)... NXTD and NXTDW) a biometric authentication company focused on ... Wocket™ smart wallet at CES 2015 in Las Vegas ... the "11 Hot Products at CES" in a review published in Wired.com, ... Newseveryday.com and "The top 10 gadgets from CES 2015" by Danny ...
(Date:1/22/2015)... NEW YORK , Jan. 21, 2015  Analyst Report Issued ... Credit Card Brands report showed that planet-wide, transactions at merchants ... 2011, while the total number of credit, debit, and prepaid ... the U.S. were victims of fraudulent card usage in 2012, ...
Breaking Biology News(10 mins):BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3$4.80 Near Term Price Target with "Buy" given by CapRock Research for NXT-ID, Inc. (NASDAQ: NXTD) issued by Small Cap IR 2
... you observe in another but have never actually experienced yourself? ... and answer this question. The research, published by Cell Press ... , provides insight into brain mechanisms involved in empathy. ... subjects feel their own emotions or observe the same emotions ...
... CAREFREE, A.Z. - With new evidence emerging on the ... that contribute to disparities in risk and outcome for ... Research will host the Science of Cancer Health Disparities ... 2009. "The goals of this conference are ...
... a Wide Range of Platforms SUNNYVALE, Calif., Jan. 27 ... Inc. , a leading supplier of innovative computer products ... today announced the development of new security technology to ... the Opal Security Subsystem Class (Opal SSC). Opal SSC ...
Cached Biology News:I feel your pain: Neural mechanisms of empathy 2Fujitsu Develops HDD Security Technology Based on Opal SSC Standards 2Fujitsu Develops HDD Security Technology Based on Opal SSC Standards 3
... The loxP-cm-loxP cassette ... in prokaryotic cells. The prokaryotic ... chloramphenicol resistance is a slightly modified ... mediates higher transcription efficiency than the ...
Includes a choice of any 3 EasySep mouse selection kits and EasySep magnet...
... Complete kit for stabilizing 1.5mg of purified ... optimised for the stabilization of aqueous RNA ... methods such as guanidine, phenol/chloroform, silica, oligo ... StabMRT Kits contain all the necessary components ...
Human SEAP ORF, provided in a pORF plasmid. pORF is an expression plasmid selectable with Ampicillin....
Biology Products: