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TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

Discussion

The TripleMaster PCR System amplified the most robust PCR products in all cases, easily outperforming the competition. Roche, the closest competitor, has one system for amplification of targets 5 kb 20 kb in size and another system for amplification of targets greater than 20 kb. However, the Eppendorf TripleMaster PCR System can amplify targets across this entire range so scientists only need to purchase one system for all long-range PCR applications. This enables researchers to save valuable time and money when ordering a PCR system for long-range PCR. Furthermore, the TripleMaster PCR System contains a straightforward, easy to use protocol that recommends specific reagent concentrations instead of a broad range of concentrations, avoiding the need for time-consuming optimization experiments. The TripleMaster System is also designed to amplify long targets up to 50 kb without the need for co-solvents or additives such as betaine or DMSO, further simplifying the master mix set-up. In addition, compared with all the systems tested, the TripleMaster PCR System used the least amount of enzyme per reaction, proving that it not only generates PCR products with the greatest yield, it is also the most cost-effective system for long-range PCR. One can see that the Eppendorf TripleMaster PCR System is the ideal choice for long-range PCR.5

References:

  1. Barnes, W.M. (1994) PCR amplification of up to 35 kb DNA with high fidelity and high yield from l bacteriophage templates. Proc. Natl. Acad. Sci. USA. 91: 2216-2220.
  2. Cantor, C.R. and C.L. S
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