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TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

15 min. 17x 93C 15 sec. 17x 65C 30 sec. 17x 68C 15 min. 20 sec. Results

The Eppendorf TripleMaster PCR System and the Roche PCR Systems amplified all three long-range PCR targets, whereas the Invitrogen and Stratagene systems did not amplify any targets (see Fig. 1). In addition, the Eppendorf TripleMaster PCR System amplified PCR products with a yield superior to the Roche PCR Systems. The U-shaped bands observed in some samples are due to the use of a glycerol-based loading buffer that allowed some of the DNA to stream up the sides of the wells during electrophoresis. Furthermore, due to the equal amount of reaction volume loaded on the gel for each competitors samples, the Eppendorf samples were overloaded, resulting in trailing and smearing. This problem was accentuated by the large size of the DNA fragments.

Fig. 1: Long-range PCR results comparing the TripleMaster PCR System to leading suppliers.
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M Bio-Rad 8 kb 48 kb marker 13 Eppendorf reactions 46 Invitrogen reactions 79 Roche reactions 1012 IStratagene reactions
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