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TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

2 U 2 U 2 U Template DNA 100 ng 100 ng 100 ng Stratagene EXL Human Factor VIII gene 29 kb Human tPA gene 24 kb Human tPA gene 18 kb EXL Buffer 1x 1x 1x Stabilizing solution 1 l 1 l 1 l Magnesium Not listed Not listed Not listed dNTP's 500 M 500 M 500 M Forward primer 200 ng 200 ng 200 ng Reverse primer 200 ng 200 ng 200 ng EXL DNA Polymerase 5 U 5 U 5 U Template DNA 250 ng 250 ng 250 ng Roche Expand 20 kbPlus & Expand Long Human Factor VIII gene 29 kb Human tPA gene 24 kb Human tPA gene 18 kb Buffer 1x 1x 1x Magnesium 2.25 mM 2.25 mM 2.25 mM dNTP's 500 M 500 M 500 M Forward primer 0.4 M 0.4 M 0.3 M Reverse primer 0.4 M 0.4 M 0.3 M
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Related biology technology :

1. TripleMaster PCR System
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4. TripleMaster PCR System: Direct Comparison with Competitors
5. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
8. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
9. Choice of RT-PCR Enzymes
10. Choice of reverse transcription primers
11. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
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