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TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

T CC 3' Table 2: Final reagent concentrations for long-range PCR reactions. Eppendorf TripleMaster Human Factor VIII gene 29 kb Human tPA gene 24 kb Human tPA gene 18 kb Tuning Buffer 1x 1x 1x Magnesium 2.5 mM 2.5 mM 2.5 mM dNTP's 500 M 500 M 500 M Forward primer 0.4 M 0.4 M 0.4 M Reverse primer 0.4 M 0.4 M 0.4 M TripleMaster enzyme mix 2 U 2 U 2 U Template DNA 500 ng 500 ng 500 ng Invitrogen ThermalAce Human Factor VIII gene 29 kb Human tPA gene 24 kb Human tPA gene 18 kb ThermalAce Buffer 1x 1x 1x Magnesium 1.5 mM 1.5 mM 1.5 mM dNTP's 200 mM @@200 M 200 mM @@200 M 200 mM @@200 M Forward primer 100 ng 100 ng 100 ng Reverse primer 100 ng 100 ng 100 ng ThermalAce DNA Polymerase
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... Reagent , 2 x 50 ml 10X TBST Wash ... 0.2 ml BL21 glycerol stock , 0.2 ml BLT5403 ... 1010 pfu T7Select Positive Control Lysate , 1010 ... HRP Conjugate , 2 ml T7Select S-protein , ...
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