Navigation Links
TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

for scientists seeking to overcome the limitations of standard PCR systems.1, 2, 3, 4

The TripleMaster PCR Systems Tuning Buffer has a unique zwitterionic formulation (patent pending) that adequately maintains pH at high temperatures (72 0C 94 0C), reducing template and amplicon degradation which is caused by depurination to a minimum. In addition, the TripleMaster Enzyme Mix combines the efficiency of Taq DNA Polymerase with the 3 5 exonuclease activity of a proofreading thermostable enzyme, along with a polymerase-enhancing factor that provides an extremely high extension rate and maximal proofreading assisted fidelity.5

Materials and Methods

Eppendorf TripleMaster PCR System
Invitrogen ThermalAceTM DNA Polymerase Kit
Stratagene EXLTM DNA Polymerase Kit
Roche Expand Long Template PCR System
Roche Expand 20 kbPlus PCR System
Genomic DNA isolated using the Eppendorf Perfect gDNA Blood Mini Kit
Eppendorf Mastercycler Gradient Thermal Cycler
FIGE Mapper Electrophoresis System (Bio-Rad)
OmniPur Agarose (EM Science)
0.5x TBE (Eppendorf)
Ethidium Bromide (Sigma)

The performance of the TripleMaster PCR System for long-range PCR was tested against other PCR systems specifically designed for long-range PCR. A 29 kb fragment of the human Factor VIII gene and 24 kb and 18 kb fragments of the human tPA gene were amplified in parallel reactions. All reactions were treated and set up in accordance with the manufacturers recommended specifications. For the Roche systems, the Expand 20 kbPlus PCR System was used to amplify the 29 kb and 24 kb targets and the Expand Long Template PCR System
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. TripleMaster PCR System
2. TripleMaster PCR System
3. The TripleMaster PCR System for three different amplifications
4. TripleMaster PCR System: Direct Comparison with Competitors
5. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
8. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
9. Choice of RT-PCR Enzymes
10. Choice of reverse transcription primers
11. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR SystemThe Ideal Choice for Long Range PCR

(Date:7/29/2014)... be separated from its properties? On July 29, the ... of the first Cheshire Cat experiment, separating a neutron ... Orange, CA, and Vienna University of Technology. , Chesire ... thought Alice in Wonderland, "but a grin without a ... in my life!" Alice,s surprise stems from her experience ...
(Date:7/29/2014)... new way of manufacturing microstructured surfaces that have ... of carbon nanotubes, could exhibit a variety of ... strength, or the ability to repel water in ... mechanical forces can be used to direct nanostructures ... can independently control the mechanical properties of ...
(Date:7/29/2014)... 2014  Pomerantz LLP is investigating claims on behalf ... "Company") (NASDAQ: IPXL).  Such investors are advised to contact ... or 888-476-6529, ext. 237. The investigation concerns ... have violated Sections 10(b) and 20(a) of the Securities ... 29, 2014, Impax Laboratories, Inc. (the "Company") issued a ...
(Date:7/29/2014)... 29, 2014 According to the ... Imaging), Analytics (Image Analysis Software), Delivery Modes (Web Based/Cloud ... & Trends to 2018", published by MarketsandMarkets, analyzes and ... such as North America , ... and the Rest of the World (RoW). ...
Breaking Biology Technology:The quantum Cheshire cat: Scientists separate a particle from its properties 2The quantum Cheshire cat: Scientists separate a particle from its properties 3The quantum Cheshire cat: Scientists separate a particle from its properties 4A new way to make microstructured surfaces 2SHAREHOLDER ALERT: Pomerantz Law Firm Investigates Claims On Behalf of Investors of Impax Laboratories, Inc. - IPXL 2Digital Pathology Market worth $437 Million by 2018 2Digital Pathology Market worth $437 Million by 2018 3
... 4, 2012  23andMe, a leader in personal genetics, has ... are the 10 most interesting and significant genetic findings ... understand the role of genetics in personal health and ... ) "The genetics revolution is well underway ...
... 2012 Luminex Corporation (NASDAQ: LMNX) today announced that ... Healthcare Conference to be held January 9 -12, 2012, ... ) The investor presentation by Patrick J. ... 1:30 p.m. Pacific time (4:30 p.m. Eastern time) on Wednesday, ...
... Jan. 4, 2012  Telik, Inc. (Nasdaq: TELK) today announced ... corporate update at the 5th Annual OneMedForum Conference on ... p.m. Eastern time) in San Francisco.  A live and ... the Telik website, www.telik.com .  The archived broadcast ...
Cached Biology Technology:23andMe Presents Top Ten Most Interesting Genetic Findings of 2011 223andMe Presents Top Ten Most Interesting Genetic Findings of 2011 323andMe Presents Top Ten Most Interesting Genetic Findings of 2011 423andMe Presents Top Ten Most Interesting Genetic Findings of 2011 523andMe Presents Top Ten Most Interesting Genetic Findings of 2011 623andMe Presents Top Ten Most Interesting Genetic Findings of 2011 7Luminex Corporation to Present at JP Morgan Healthcare Conference 2
(Date:7/30/2014)... 30, 2014 ResearchMoz presents ... Biometric Systems Market 2014-2024   Business Strategies, Industry Trends, ... Systems Market 2014-2024" offers the reader detailed analysis ... next ten years, alongside potential market opportunities to ... To view the table of contents ...
(Date:7/30/2014)... July 30, 2014 NexID Biometrics ... liveness detection solutions for the biometric authentication industry, ... consortium revolutionizing online authentication with standards for strong ... , "The decision to join FIDO Alliance stems ... pervasive in use. We believe the FIDO Alliance ...
(Date:7/30/2014)... ranges and a changing climate threaten some of ... and so conservationists establish seed collections to save ... hopes of preserving some genetic diversity., For decades, ... models that offer a one-size-fits-all approach for how ... 50 seed samples regardless of species, pollination mode, ...
Breaking Biology News(10 mins):Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 2Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 3Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 4Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 5Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 6Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 7Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 8Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 9Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 10NexID Biometrics Joins the FIDO Alliance 2Saving seeds the right way can save the world's plants 2
... results from an ambitious voyage led by a group of ... Diego offer a stark view of human pollution and its ... labeled as the "Great Pacific Garbage Patch." Two graduate ... SEAPLEX, found evidence of plastic waste in more than nine ...
... researchers have produced a map of Edmonton predicting the most ... can be fatal for drivers and their passengers. The hot ... the city limit, border line. Mark Boyce is a ... Boyce found that the most dangerous rural roadways share three ...
... are incredibly complex others manage without. Researchers at the ... their entire body despite having no eyes at all. The ... of the National Academy of Sciences (PNAS). Most ... way of seeing complex images so that they can function ...
Cached Biology News:Scripps study finds plastic in 9 percent of 'garbage patch' fishes 2Scripps study finds plastic in 9 percent of 'garbage patch' fishes 3Researchers predict locations for deer vs. car collisions 2
...
... Acid) Clear to very pale yellow ... 2 O 2 in a citrate buffer ... is soluble, safe, convenient and has good overall ... blue-green color that can be measured at 405 ...
...
...
Biology Products: