Navigation Links
TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

for scientists seeking to overcome the limitations of standard PCR systems.1, 2, 3, 4

The TripleMaster PCR Systems Tuning Buffer has a unique zwitterionic formulation (patent pending) that adequately maintains pH at high temperatures (72 0C 94 0C), reducing template and amplicon degradation which is caused by depurination to a minimum. In addition, the TripleMaster Enzyme Mix combines the efficiency of Taq DNA Polymerase with the 3 5 exonuclease activity of a proofreading thermostable enzyme, along with a polymerase-enhancing factor that provides an extremely high extension rate and maximal proofreading assisted fidelity.5

Materials and Methods

Eppendorf TripleMaster PCR System
Invitrogen ThermalAceTM DNA Polymerase Kit
Stratagene EXLTM DNA Polymerase Kit
Roche Expand Long Template PCR System
Roche Expand 20 kbPlus PCR System
Genomic DNA isolated using the Eppendorf Perfect gDNA Blood Mini Kit
Eppendorf Mastercycler Gradient Thermal Cycler
FIGE Mapper Electrophoresis System (Bio-Rad)
OmniPur Agarose (EM Science)
0.5x TBE (Eppendorf)
Ethidium Bromide (Sigma)

The performance of the TripleMaster PCR System for long-range PCR was tested against other PCR systems specifically designed for long-range PCR. A 29 kb fragment of the human Factor VIII gene and 24 kb and 18 kb fragments of the human tPA gene were amplified in parallel reactions. All reactions were treated and set up in accordance with the manufacturers recommended specifications. For the Roche systems, the Expand 20 kbPlus PCR System was used to amplify the 29 kb and 24 kb targets and the Expand Long Template PCR System
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. TripleMaster PCR System
2. TripleMaster PCR System
3. The TripleMaster PCR System for three different amplifications
4. TripleMaster PCR System: Direct Comparison with Competitors
5. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
8. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
9. Choice of RT-PCR Enzymes
10. Choice of reverse transcription primers
11. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR SystemThe Ideal Choice for Long Range PCR

(Date:12/15/2009)... Force Microscopy (AFM) have been developed and are opening up ... interface. The NeedleProbes’ cylindrical geometry enables researchers to measure ... - all in a single force distance curve. , ... ... constant diameter NeedleProbes are enabling unique measurements at ...
(Date:12/15/2009)... PHILADELPHIA and LONDON, Dec. 15 ... Thomson Pharma ® has become the first ... trial protocol and outcome information. This highly anticipated ... Pharma customers this month. ,, ... trial intelligence from registries, publications, press releases, conferences, ...
(Date:12/14/2009)... Dyadic International, Inc. (Pink Sheets: DYAI) ("Dyadic") announced ... The Scripps Research Institute ("Scripps") by entering into ... of Dyadic,s patented and proprietary C1 fungus ("C1"). ... successful relationship between the two organizations which began ... annotate the C1 genome for the first time. ...
(Date:12/14/2009)... Nektar Therapeutics (Nasdaq: NKTR ) ... to serve on its board of directors. ... over 35 years of experience in corporate management, ... Nektar extensive industry perspective at an important time ... President and CEO of Nektar and member of ...
Breaking Biology Technology:New Constant Diameter NeedleProbes™ with Unique Ability to Measure Liquid Surface Properties by AFM 2New Constant Diameter NeedleProbes™ with Unique Ability to Measure Liquid Surface Properties by AFM 3Thomson Reuters is First to Offer Fully Integrated Pipeline and Clinical Trials 2Dyadic International Extends Collaboration With The Scripps Research Institute 2Dyadic International Extends Collaboration With The Scripps Research Institute 3Dyadic International Extends Collaboration With The Scripps Research Institute 4Dyadic International Extends Collaboration With The Scripps Research Institute 5Dennis Winger Joins Nektar Therapeutics' Board of Directors 2Dennis Winger Joins Nektar Therapeutics' Board of Directors 3
... TUSTIN, Calif., Oct. 22 AMDL, Inc. ... in Shenzhen,Jiangxi and Jilin, China, through its ... an international biopharma company that,engages in the ... diagnostic products. AMDL announced today that its,management ...
... Bioscience announced today that it has been awarded ... from the United,States Defense Threat Reduction Agency (DTRA) ... a broad spectrum therapeutic for the treatment,of a ... is melioidosis which is the most common cause ...
... LYNBROOK, N.Y., Oct. 20 BioSpecifics,Technologies Corp. (Pink Sheets: ... an overview of the company and it,s product,pipeline at ... Rodman,Renshaw LLC. The presentation will take place on Wednesday ... Palace Hotel in Holmes I. About BioSpecifics Technologies ...
Other Biology Technology:AMDL to Present its China Success Story at Rodman & Renshaw 9th Annual Healthcare Conference 2Implicit Awarded US $16.3 Million U.S. Government Contract to Develop Immunomodulatory Drug for Biodefense Threats 2
(Date:12/13/2009)... 10, 2009Researchers at The Scripps Research Institute have ... protein from an ancient family of enzymes can ... In addition to providing guidance for understanding other ... theoretical underpinning for the protein,s possible use for ... The research was published in the December 13, ...
(Date:12/11/2009)... increases, growers are being challenged to bring consistently ... bottom line, orchard owners are experimenting with new ... labor costs. , Hand thinning, a common practice ... is among the most labor-intensive of orchard practices, ... and, ultimately, prices paid by consumers. A research ...
(Date:12/11/2009)... children understand that green plants photosynthesize, absorb carbon ... profound global-scale effects that photosynthesis has had on ... the origins and evolution of photosynthesis is Jeffrey ... of Life Sciences. , Oxygen, one of the ... and their descendants (including algae and higher plants), ...
Breaking Biology News(10 mins):Scripps Research scientists crack mystery of protein's dual function 2Scripps Research scientists crack mystery of protein's dual function 3Horizontal string trimmer reduces labor costs, increases peach size 2'Extreme' genes shed light on origins of photosynthesis 2'Extreme' genes shed light on origins of photosynthesis 3Labopharms response for novel trazodone formulation accepted by FDA as complete Designated as Class 2 resubmission 55334 1Labopharms response for novel trazodone formulation accepted by FDA as complete Designated as Class 2 resubmission 55334 2International Travel Raises Risk of Typhoid 55331 1International Travel Raises Risk of Typhoid 55331 2Frank Sadeghi Joins CBCA Administrators Inc as Executive Vice President of Business Development 55326 1Frank Sadeghi Joins CBCA Administrators Inc as Executive Vice President of Business Development 55326 2
... attempt to better understand the Klamath River basin ecosystem. ... Council says for the studies to prove more useful ... should be completed to identify all research and management ... to help reconstruct pre-development flows -- without dams or ...
... nanotechnologys potential risks growing" If so, who ... and newswiresenvironmental and consumer organizations, scientists, law makers, ... journalists decide to cover a nanotechnology story, especially ... television correspondents face special challenges reporting on a ...
... of fuel cell powered with glucose derived from biomass ... Publication International Journal of Global Energy Issues. The experimental ... into hydrogen to power the cell, which produces several ... as food waste and managed high-energy crops, are gradually ...
Other Biology News:More comprehensive analysis of Klamath River basin needed to aid decision makers 2More comprehensive analysis of Klamath River basin needed to aid decision makers 3More comprehensive analysis of Klamath River basin needed to aid decision makers 4Nanotechnology and the media: The inside story 2
... ClearSpin Filter is a novel disposable spin ... clarification of bacterial lysates in plasmid purification ... a novel, disposable spin filtration device especially ... lysates in plasmid purification procedures. The neutralized ...
... standard fixed-angle microcentrifuge rotor at RCF ... tube filters consist of a membrane-containing ... They filter by centrifugation for bacteria ... removal of cells from media and ...
... module C, 0.7 mm depth • Reusable, ... can be quickly assembled and disassembled for ... specimens of any thickness with up to ... three chambers per slide. Minimize specimen handling, ...
12 breakable polypropylene strips of 8 wells each held in a rigid frame. Ideal for antibody assays....
Biology Products: