10x HighFidelity Buffer with Mg2+ 1.5 ml 1.5 ml 2 x 1.5 ml 25 mM Magnesium Solution 1.5 ml 1.5 ml 1.5 ml Also available is the TripleMaster PCR System Plus. It includes the above components as well as: 20 l of Control DNA (10 ng/l) and 20 l of Control Primer Mix (10 pmol/l each), enough for 20 control reactions. 3.2 Materials required but not supplied

Template DNA
Primers
dNTPs*
Molecular Biology Grade Water*
*Products available through Eppendorf

4 Enzyme concentration

5 U/l*
*concentration refers to the amount of Taq DNA polymerase per l of the TripleMaster Polymerase Mix, although the specific activity (by nucleotide incorporation) of Taq in combination with the other enzymes is higher than the specific activity of pure Taq of the same concentration.

5 Enzyme storage buffer composition

TripleMaster Polymerase Mix: 20 mM Tris-HCl pH 8.0 (at 25C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol, 0.5 % Tween 20, 0.5 % Igepal CA-630.

6 Storage and stability

Store all reagents of the TripleMaster PCR System at 20C in a constant-temperature freezer. If stored as recommended, the kit is stable at least until the expiration date stated on the label.

7 Quality assurance

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