Navigation Links
TripleMaster PCR System

ogous to PCR products amplified by Taq DNA polymerase. TAcloning is therefore recommended. For blunt-end cloning, the fragments need to be polished by Klenow enzyme or T4 DNA polymerase.

11.8 Sequencing

Sequencing with the TripleMaster Polymerase Mix is not recommended due to the inherent 35 exonuclease activity.

11.9 Primer sequences for long targets

universal forward primer (in the control primer mix) :
5-CTGATGAGTTCGTGTCCGTACAACTGGCGTAATC-3 reverse primers: 40 kb: 5-TAATGCAAACTACGCGCCCTCGTATCACATGG-3 (in the control primer mix) 35 kb: 5-ATTATGTCGGTGATACTTCGTCGCTGTCTC-3 30 kb: 5-GAAAGTTATCGCTAGTCAGTGGCCTGAAGAGACG-3 20 kb: 5-GTGCACCATGCAACATGAATAACAGTGGGTTATC-3 10 kb: 5-ATACGCTGTATTCAGCAACACCGTCAGGAACACG-3 universal forward primer for human tPA gene: 5-CCTTCACTGTCTGCCTAACTCCTTCGTGTGTTCC-3 reverse primer for human tPA gene: 15 kb: 5- ACTGTGCTTCCTGACCCATGGCAGAAGCGCCTTC-3 18 kb: 5-GCAGGGGTGCTGCAGAACTCTGAGCTGTACTTCC-3 22 kb: 5-GATGCGAAACTGAGGCTGGCTGTACTGTCTC-3 24 kb: 5-TGTCTCCAGCACACAGCATGTTGTCGGTGAC-3
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. TripleMaster PCR System
2. The TripleMaster PCR System for three different amplifications
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR System

(Date:7/10/2014)... July 10, 2014 Senior supply chain ... professional associations, addressed the challenges of “Reducing Cost, Lead ... Industries” through strategic sourcing. Describing the partnership of the ... Supply Chain Management Institute ( SCMI) of the ... June 24 at the Kroc Institute of Peace and ...
(Date:7/10/2014)... , July 10, 2014  Kainos Capital, a firm ... today announced that it has acquired the Slim-Fast brand ... the business. Terms of the transaction were not disclosed. ... meal replacement business that markets ready-to-drink shakes, powders, bars ... America and in the United ...
(Date:7/10/2014)... July 10, 2014   Ceres, Inc . (Nasdaq: ... company, announced today financial results for the three months ... its business. Ceres reported that the company ... performance this growing season in Brazil, which concluded in ... company,s sorghum evaluation areas for part of the growing ...
(Date:7/10/2014)... microscopic polymer light resonators that expand in the ... Photonics Laboratory have developed new optical sensors with ... sensors are ideal for detecting trace gas concentrations ... nature, and immunity to electromagnetic interference. , Although ... the MIT team conceived an extremely sensitive, compact ...
Breaking Biology Technology:Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4Kainos Capital Acquires Slim-Fast From Unilever 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9Swell new sensors 2
... 4 Cerimon,Pharmaceuticals, Inc. announced today that Matthew ... established position, and Vice President of,Business Development and ... and corporate attorney with broad experience with large,pharmaceutical, ... and,Europe. In the business development role, he replaces ...
... Approved for Marketing in the United States, Trade Name Will be ... ... Daiichi Sankyo,Company, Limited (TSE: 4568) and Eli Lilly and Company (NYSE: ... New Drug,Application (NDA) for prasugrel to the U.S. Food and Drug Administration,(FDA). ...
... Inc.,(Nasdaq: THRM ), a leader in non-invasive ... it will present at the 10th Annual Needham ... New York City. The,Company,s presentation is scheduled for ... Stephen J. Fanning, Chairman, President and Chief,Executive Officer, ...
Cached Biology Technology:Cerimon Pharmaceuticals Names Matthew J. Meyer its General Counsel and Vice President of Business Development and Licensing 2Daiichi Sankyo, Lilly Submit New Drug Application for Investigational Antiplatelet Drug, Prasugrel, with U.S. Food and Drug Administration 2Daiichi Sankyo, Lilly Submit New Drug Application for Investigational Antiplatelet Drug, Prasugrel, with U.S. Food and Drug Administration 3Daiichi Sankyo, Lilly Submit New Drug Application for Investigational Antiplatelet Drug, Prasugrel, with U.S. Food and Drug Administration 4
(Date:7/11/2014)... from The Chinese University of Hong Kong, BGI and ... linked to salt tolerance, with implication for improving this ... published online in Nature Communications provides an ... improvement. , Soybean is an important crop for the ... have less genetic diversities than their wild counterparts. Among ...
(Date:7/11/2014)... other group members to reduce aggression and gain access ... for grooming activities shows a certain pattern across the ... journal Biology Letters . , Grooming between individuals ... ulterior motives. To be groomed has hygienic benefits and ... individual can provide access to infants, mating opportunities and ...
(Date:7/11/2014)... University, Sweden, have explored two different ways that ... leading to the formation of oxygen molecules in ... the scientific journal Nature Communications . , ... synthetic catalysts for water oxidation, which are key ... direct storage of solar energy in fuels like ...
Breaking Biology News(10 mins):BGI reports a novel gene for salt tolerance found in wild soybean 2Baboons groom early in the day to get benefits later 2Molecular snapshots of oxygen formation in photosynthesis 2
... cold groundwater close to hot groundwater, and controlling mineral ... University of Catalonia and the University of Barcelona, after ... all by arsenic pollution. In this region, which is ... well as in other Catalan coastal mountain ranges, uranium ...
... Fla. --- The discovery of two new extinct camel species ... history of the tropics, a region containing more than half ... Appearing online this week in the Journal of ... of a fossil mammal discovered as part of an international ...
... to lead wreaks havoc in the brain, with consequences ... But the precise mechanism by which lead alters nerve ... New research led by Toms R. Guilarte, PhD, Leon ... Columbia University Mailman School of Public Health, and post-doctoral ...
Cached Biology News:Contamination of La Selva geothermal system in Girona, Spain 2Contamination of La Selva geothermal system in Girona, Spain 3UF scientists name new ancient camels from Panama Canal excavation 2UF scientists name new ancient camels from Panama Canal excavation 3In what ways does lead damage the brain? 2
...
Cag-A (IgG) -Ab EIA Sample Size: 10 l...
... RNaseOUT Recombinant Ribonuclease Inhibitor is ... pancreatic-type ribonucleases such as RNase ... RNaseOUT Recombinant Ribonuclease Inhibitor is ... molecular weight ~52 kDa. RNaseOUT ...
... RNase Inhibitor (Ribonuclease inhibitor) is ... used to inhibit RNase activity. ... or endonuclease activity. , ... of RNA template , ...
Biology Products: