Navigation Links
TripleMaster PCR System

dNTP / Mg2+ Long range PCR: 500 M / 2.5 mM High fidelity PCR: 200 M / 2.5 mM (optional 80150 M/2.5 mM) PCR of GC-rich targets: 250500 M / 2.5 mM 11.5 Dilution of the TripleMaster Polymerase Mix

Prepare a 1:10 enzyme dilution as follows:
Add 32 l Molecular Biology Grade Water and 4 l of the corresponding 10x reaction buffer to a sterile microcentrifuge tube, mix well and spin down. Then add 4 l of the TripleMaster Polymerase Mix, mix thoroughly (glycerol solution) and spin down quickly. Keep the enzyme dilution on ice until use. It is stable for several hours. Do not store overnight!

11.6 Fidelity

In combination with both reaction buffers, the TripleMaster Polymerase Mix exhibits a 45 fold higher fidelity (2.3 x 10-6) than Taq DNA polymerase (12.5 x 10-6). The TripleMaster PCR System keeps the same high fidelity for long range amplification of large genomic targets (> 20 kb) as for amplification of short targets, because it circumvents the use of fidelityreducing co-solvents such as DMSO. Note: The addition of 5 % DMSO to the PCR causes a reduction of polymerase fidelity by more than 50 %. The additon of 10 % DMSO reduces the fidelity of proofreading polymerase blends to the level of Taq DNA polymerase alone.

11.7 Cloning

PCR products generated by the TripleMaster PCR System contain a mixture of fragments with blunt ends and 3-single nucleotide overhangs anal
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. TripleMaster PCR System
2. The TripleMaster PCR System for three different amplifications
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR System

(Date:9/30/2014)... . , Electrical ... a new kind of building block for digital integrated ... be based on three-dimensional arrangements of nanometer-scale magnets instead ... semiconductor industry CMOS fabrication of silicon chips ... the University of Notre Dame are exploring "magnetic computing" ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 Shimadzu ... extensometer, an easy-to-use and highly precise tool for ... with considerable elongation. With 1000-mm maximum movement distance, ... percent for a gauge length of 10 mm. ... measurement accuracy above 50-mm stroke and within +/- ...
(Date:9/30/2014)... September 30, 2014 Follow us ... of each type of cholesterol carrier or lipoprotein in ... of excessive cholesterol levels in the body, especially along ... to heart attack and other cardiovascular disorders. Cardiovascular Diseases ... testing for cholesterol levels on a regular basis, to ...
(Date:9/30/2014)... 30, 2014 Genedata, a ... drug discovery and related life science research, ... chosen Genedata Selector as its host-pathogen data ... FUNGITECT is a European consortium dedicated to ... via individualized anti-fungal drug therapies. Genedata Selector ...
Breaking Biology Technology:A new dimension for integrated circuits: 3-D nanomagnetic logic 2A new dimension for integrated circuits: 3-D nanomagnetic logic 3Shimadzu’s New Long-Travel Extensometer Increases Precision for Soft Material Testing 2Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 2Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 3Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 4FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 2FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 3
... , , , Genetic reporter ... regulatory promoter and enhancer sequences as well as transcription factors. , ... enhancer) is cloned , together with the reporter ... used to transfect cells. Quantification of the reporter indirectly provides , ...
... , , , , Using eukaryotic ... to a hypoosmolar buffer system and short pulses in the s-range. , ... of sample resistance (patent pending)., , , ... Two cell lines (mouse fibroblast cells, human T-lymphocytes) were transfected ...
... , , , , , , PLG can be used ... The basic protocol given here works with E. coli cultures grown for 1214 , ... , ml Terrific Broth. , , ... 10,000 x g for 5 minutes at room , temperature. ...
Cached Biology Technology:Genetic Reporter Systems 2Genetic Reporter Systems 3Genetic Reporter Systems 4Basic Plasmid DNA Isolation Protocol 2
(Date:10/1/2014)... October 1, 2014 ... der Biowissenschaften   Die Biowissenschaften erlebten ... Gehen zahlreicher Detektionstechnologien. Einige davon sind Senkrechtstarter ... Die Photonen-Upconversion ist eine neuartige Detektionstechnologie, die ... Begeisterung aufgenommen wurde. Jetzt ist diese Technologie ...
(Date:9/30/2014)... visit the Mediterranean each year, but its deep-blue ... Earth. Almost 1,000 alien species, including fish, crustaceans, ... through human activities. In the open-access journal ... of researchers analyzed data from a new information ... how the introduction of alien species has changed ...
(Date:9/30/2014)... as something that we enjoy when we visit a national ... think of ourselves as a part of nature? A bird,s ... house? , The answers to these questions reflect different ... speech and in cultural artifacts. , A new Northwestern University ... Indian Center of Chicago and the Menominee tribe of Wisconsin, ...
Breaking Biology News(10 mins):Innovative Verwendungslösung für die Photonen-Upconversion 2Innovative Verwendungslösung für die Photonen-Upconversion 3Biodiversity in the Mediterranean is threatened by alien species 2The cultural side of science communication 2
... University professor of physics and astronomy Apparao Rao has ... Fellows are elected by their peers for outstanding contributions ... one half of 1 percent of the membership. ... nanotubes and for elucidating the properties of carbon nanotubes ...
... PITTSBURGHUniversity of Pittsburgh researchers have developed the first ... finding that could help diminish the environmental and ... of the super-strong materials commonly used in products, ... team has found that carbon nanotubes deteriorate when ...
... 16, 2008) Researchers from Virginia Commonwealth University have identified ... and suppress a key protein that is overexpressed in 90 ... to an effective gene therapy for cancer. According ... the Department of Human and Molecular Genetics and director of ...
Cached Biology News:Pitt researchers create non-toxic clean-up method for potentially toxic nano materials 2Researchers identify new anti-tumor gene 2
... Microplate Rack inserts fit into ... and allow you to greatly ... shake deepwell format plates at ... plates from the shaker at ...
BD FastImmune EDTA Solution. Applications: Flow Cytometry Storage Temperature: Refrigerate(2 to 8C)...
Spaced cover glasses to promote effective hybridization...
prototype Nco I...
Biology Products: