Navigation Links
TripleMaster PCR System

dNTP / Mg2+ Long range PCR: 500 M / 2.5 mM High fidelity PCR: 200 M / 2.5 mM (optional 80150 M/2.5 mM) PCR of GC-rich targets: 250500 M / 2.5 mM 11.5 Dilution of the TripleMaster Polymerase Mix

Prepare a 1:10 enzyme dilution as follows:
Add 32 l Molecular Biology Grade Water and 4 l of the corresponding 10x reaction buffer to a sterile microcentrifuge tube, mix well and spin down. Then add 4 l of the TripleMaster Polymerase Mix, mix thoroughly (glycerol solution) and spin down quickly. Keep the enzyme dilution on ice until use. It is stable for several hours. Do not store overnight!

11.6 Fidelity

In combination with both reaction buffers, the TripleMaster Polymerase Mix exhibits a 45 fold higher fidelity (2.3 x 10-6) than Taq DNA polymerase (12.5 x 10-6). The TripleMaster PCR System keeps the same high fidelity for long range amplification of large genomic targets (> 20 kb) as for amplification of short targets, because it circumvents the use of fidelityreducing co-solvents such as DMSO. Note: The addition of 5 % DMSO to the PCR causes a reduction of polymerase fidelity by more than 50 %. The additon of 10 % DMSO reduces the fidelity of proofreading polymerase blends to the level of Taq DNA polymerase alone.

11.7 Cloning

PCR products generated by the TripleMaster PCR System contain a mixture of fragments with blunt ends and 3-single nucleotide overhangs anal
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. TripleMaster PCR System
2. The TripleMaster PCR System for three different amplifications
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR System

(Date:10/20/2014)... 20, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the ... Q3 orders to $5.8 million and provides a good start to ... North America and one in the ... projects at record levels," said Peter Bruijns , President & ... end of Q3 than they have been for any complete year ...
(Date:10/20/2014)... Inc. (NASDAQ: GNVC ) today announced the retirement ... of directors effective on October 24, 2014.   Dr. Horovitz joined ... chairman from June 2006 to November 2013.  During his tenure, ... Audit Committees of the board.  "We deeply ... to GenVec, and its stockholders," said Wayne T. Hockmeyer ...
(Date:10/20/2014)... (PRWEB) October 20, 2014 ... Sidney Kimmel Cancer Center at Thomas Jefferson University ... , Through the Strategic Alliance Partnership program, the ... will collaborate to raise awareness of the Center’s ... and other projects. Clinicians and other health care ...
(Date:10/20/2014)... Local veterinary surgeon, Dr. Tim McCarthy ... of donor stem cells for dogs with osteoarthritis. Dr. ... has performed clinical stem cell therapy for 7 years. ... if a single injection of donor stem cells into ... pain and inflammation in the treated joints. , ...
Breaking Biology Technology:Zola P. Horovitz To Retire From GenVec Board 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3Cascade Veterinary Referral Center Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2
... N.J., Feb. 9 Pharmasset, Inc. (Nasdaq: ... committed to discovering, developing and commercializing novel drugs ... financial results for the fiscal quarter ended December ... million during each of the quarters ended December ...
... Informed Medical Communications (IMC), a leading provider ... today announced the appointment of Raghu Raghunath to ... This key addition to the executive team is ... and technologically advanced solutions for its clients. ...
... ImQuest Life Sciences today presented important new results ... inhibitor of HIV-1 at the 16th CROI meeting ... the advanced development of ImQuest,s lead HIV pyrimidinedione ... of HIV, exhibiting a dual mechanism of action ...
Cached Biology Technology:Pharmasset Reports Fiscal First Quarter 2009 Financial Results 2Pharmasset Reports Fiscal First Quarter 2009 Financial Results 3Pharmasset Reports Fiscal First Quarter 2009 Financial Results 4Pharmasset Reports Fiscal First Quarter 2009 Financial Results 5Pharmasset Reports Fiscal First Quarter 2009 Financial Results 6Pharmasset Reports Fiscal First Quarter 2009 Financial Results 7Pharmasset Reports Fiscal First Quarter 2009 Financial Results 8Informed Medical Communications Appoints New Vice President, Information Technology 2ImQuest Presents IND-Enabling Safety Pharmacology and Pharmacokinetic Data for the Therapeutic HIV Compound IQP-0410 at Conference on Retroviruses and Opportunistic Infections 2
(Date:10/15/2014)... University Mainz (JGU) as a full member, ensuring ... a significant nationwide standing in the field of ... Gutenberg University Mainz as a full member of ... and international competition for the best minds and ... proud that Mainz University has been recognized as ...
(Date:10/15/2014)... , Oct. 15, 2014   Neurotechnology , ... announced the availability of the VeriLook Surveillance ... 3.0 provides real-time biometric face identification using live ... surveillance cameras. The new version not only identifies ... people from objects while they are moving through ...
(Date:10/14/2014)... the planet,s leading questions is how to produce enough ... climate. The Food and Agriculture Organization of the United ... the next 40 years to feed a growing global ... necessary rise in food production. Plants—grains, cereals, fruits, ... livestock. Current research must tap into our knowledge ...
Breaking Biology News(10 mins):Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 2Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 3VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 2VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 3Building a bridge from basic botany to applied agriculture 2Building a bridge from basic botany to applied agriculture 3
... science and technology has seen exciting advances recently ... According to international scientific conventions, nanomaterials are those ... or equal to 10-9 m. At the same ... to enter ecosystems at the points of use ...
... 2012 Individuals with mutations in BRCA1 and BRCA2 genes ... cancers. Families at risk have been seeking genetic testing ... standard method of direct sequencing is labor-intensive, costly, and it ... A group of Canadian scientists has developed a new ...
... study of North American songbirds reveals that birds that live ... up helps birds ensure that their songs are heard no ... and the National Evolutionary Synthesis Center. To test the ... male birds spanning 44 species of North American songbirds ...
Cached Biology News:Understanding the biological and ecological implications of safe nanotechnology 2New method provides fast, accurate, low cost analysis of BRCA gene mutations in breast cancer 2Birds that live with varying weather sing more versatile songs 2
EPA II Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods....
... important role in the regulation of signaling ... Splice variants have been reported that affect ... apoptotic pathways. With Apoptosis and Cell Death ... expression profiling and at the same time ...
Mouse monoclonal antibody raised against a full length recombinant CSNK2A2. NCBI Entrez Gene ID = CSNK2A2...
... Kit is manufactured for DNA purification from ... to UV light. The Purification of DNA ... for DNA-cloning work. The conventional method of ... of UV light damages DNA and significantly ...
Biology Products: