Navigation Links
TripleMaster PCR System

primer mix containing DMSO (see step 4 in protocol below and table 8). Protocol for GC-rich targets
  1. Thaw the reagents listed in Table 8 carefully and keep on ice.
  2. Make sure reagents are thoroughly thawed. Mix each reagent and briefly centrifuge before use.
  3. Set up Mastermix 1 (see Table 8) containing buffer, primer, template DNA and DMSO (optional) in a sterile microcentrifuge tube, mix well, centrifuge and heat for 30 seconds at 98C.
  4. Quickly spin down (2 sec) Mastermix 1 and immediately place on ice.
  5. Prepare Mastermix 2 (see Table 8) in a separate sterile microcentrifuge tube, mix well, centrifuge and place on ice.
  6. Just before cycling, pipette Mastermix 1 and then Mastermix 2 into a separate PCR tube on ice. Mix quickly but thoroughly, (vortex) and centrifuge.
  7. Place immediately into a thermal cycler that is pre-heated at 98C (use the "Hold" step for this) and start cycling.
  8. Analyze amplification products on a 0.6-1.5 % agarose gel using appropriate DNA markers.
Table 8: Mastermix conditions for GC-rich PCR 1 increase final buffer conc. (1.6x) for targets < 500 bp or when using a low concentration of template DNA per reaction (< 10 ng genomic DNA or < 0.5 ng plasmid DNA)
2 increase dNTP concentration for GC-rich targets

Table 9: Cycling program for GC-rich targets 1 Difficult targets require more cycles (and template DNA) for efficient amplificati
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. TripleMaster PCR System
2. The TripleMaster PCR System for three different amplifications
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR System

(Date:9/18/2014)... course of a day - car, phone, computer, ... chemical industry to turn raw materials such as ... chemicals that are the ingredients of life,s essential ... achieved outstanding progress in driving this energy intensive ... instead of fossil fuels, has won the prestigious ...
(Date:9/18/2014)... (PRWEB) September 18, 2014 Pipette.com ... include Sartorius/Biohit, Socorex, Capp, and AccuPet brand of ... of 100 uL or less and are a ... forensic applications. , The micropipette promotions were ... and economic alternative products to help customers find ...
(Date:9/17/2014)...  PDL BioPharma, Inc. (PDL) (NASDAQ: PDLI ) ... & Young LLP ("EY") that it was resigning effective September ... to the Company on September 15, 2014 and reported in ... PDL has issued the following statement in response to ... inquiries on the reason for the resignation of EY.  We ...
(Date:9/17/2014)... - Oncothyreon Inc. (NASDAQ: ONTY ) today ... of its Common Stock and Series A Convertible Preferred ... Series A Convertible Preferred Stock is non-voting and convertible ... will be prohibited if, as a result, the holder ... of the Common Stock then outstanding. As part of ...
Breaking Biology Technology:Using solar energy to turn raw materials into ingredients for every day life 2Major Pipette Distributor Pipette.com Announces Promotions on Sartorius/Biohit, Socorex, Capp, and AccuPet Micropipettes 2Major Pipette Distributor Pipette.com Announces Promotions on Sartorius/Biohit, Socorex, Capp, and AccuPet Micropipettes 3PDL BioPharma Issues Statement on Resignation of Ernst and Young as Corporate Auditors 2PDL BioPharma Issues Statement on Resignation of Ernst and Young as Corporate Auditors 3Oncothyreon Announces Proposed Public Offerings 2
... class of enzymes routinely used to synthesizing some of nature's ... a new way to expand on nature's chemical creativity to ... (Sept. 1) in the journal Science , a team ... at the University of Wisconsin-Madison , describes the discovery ...
... life sciences company with 120 Madison employees is bolstering ... of proteins in today's drug development market. , ,The ... Corp. 's recent acquisition of Sentigen Holding Corp., which ... Madison. , , The scientific opportunity , ,The ...
... summer, but for workers the lazy, hazy, crazy vacation days ... So now that we are all rested and relaxed, what ... , ,Well, actually neither workers or the workplace seem to ... conflict and strife between management and workers, real tensions exist ...
Cached Biology Technology:UW finds new path to anti-cancer agents 2UW finds new path to anti-cancer agents 3Invitrogen boosts drug development capability 2Workplace flexibility: Give and take or tug of war? 2Workplace flexibility: Give and take or tug of war? 3Workplace flexibility: Give and take or tug of war? 4Workplace flexibility: Give and take or tug of war? 5
(Date:9/17/2014)... A team of Spanish researchers have obtained the first ... a sixteenth century pig found at the site of ... that this ancient pig is closely related to today,s ... pigs were crossed with modern Iberian pigs. , The ... on evolutionary aspects of pig species, and particularly on ...
(Date:9/17/2014)... DC A team of researchers working on a Carnegie ... coral growth rates have plummeted 40% since the mid-1970s. ... an important role in this perilous slowdown. , ... facing coral reefs, the team compared current measurements of ... Barrier Reef with similar measurements taken more than 30 ...
(Date:9/17/2014)... social behavior by studying nonhuman mammals and primates, but ... their unusually large brains relative to their body size ... environment---not merely in a large population of cooperating creatures, ... setting of alliances and competitors. The same is true ... social carnivores, like hyenas and lions. , A new ...
Breaking Biology News(10 mins):Iberian pig genome remains unchanged after 5 centuries 2Coral growth rate plummets in 30-year comparison 2Being social: Learning from the behavior of birds 2
... Nov. 18, 2010 The 2010 Golf Atlanta charity golf ... aimed at finding better treatments and eventually a cure for ... 15, 2010, to the Crabapple Golf Club in Alpharetta, Ga., ... Genomics Research Institute (TGen), a non-profit biomedical research facility in ...
... For Immediate Release While scientists depend on ... long and frequently frustrating process. For one Scripps Research ... Ja, an assistant professor in the Department of Metabolism ... January, was recently awarded an unsolicited grant of $60,000 ...
... November 18, 2010 The Paul G. Allen Family ... and cellular engineering research. The Allen Distinguished Investigator Awards ... Washington, California, Massachusetts and New York. The grants ... years. "A year ago, I started ...
Cached Biology News:Golf Atlanta charity tournament raises $105,000 for TGen research 2New Scripps Florida scientist awarded pair of unconventional grants 2First Allen Distinguished Investigators named 2First Allen Distinguished Investigators named 3
... Laser Capture Microdissection (LCM) is the ... worldwide. Veritas Microdissection is the only instrument ... providing unmatched flexibility., UV Laser ... ,Ideal for non-soft tissues and capturing large ...
Hybridizer (220 V) For more information, please visit Dako' web-site. EU regulatory status: CE US regulatory status: IVD...
... System is an automated multiplexing platform that ... detection, pathogen sequence detection and pharmacogenetics applications. ... applied to an open and flexible electronic ... reused until all tests sites have been ...
The Bio-Plex Manager version 3.0 to 4.0 workstation software upgrade kit is used to upgrade existing Bio-Plex Manager version 3.0 software to version 4.0 security edition software....
Biology Products: