Navigation Links
TripleMaster PCR System

1 some applications may require an extra addition of Magnesium Solution (supplied) to set up a final concentration >2.5 mM.
2 increase final buffer conc. (1.6x) for targets < 500 bp or when using a low concentration of template DNA per
reaction (< 10 ng genomic DNA or < 0.5 ng plasmid DNA)

Cycling program for high fidelity PCR

A standard program which can be adapted to an appropriate primer/template system is shown in Table 6. The data of Table 7 may be used as a guide for the adaptation of individual PCR reactions.

Table 6: Program for high fidelity PCR with Eppendorf Mastercycler 1 Number of cycles depends on product yield required, template concentration and complexity.
2 The annealing temperature depends on the melting temperatures of the primer set used.
3 This elongation temperature is recommended for long targets >5 kb to reduce the temperature-driven damage of template DNA.
4 The elongation time depends on fragment length and type of thermal cycler. Elongation times with the Eppendorf astercycler for various fragment sizes are given in Table 7. These values can also be transferred to other Peltier-driven cyclers.

Table 7: Target size and elongation time 1 The elongation time required for targets with an unusual (non-random) nucleotide base composition, repeats or high GC-content is longer than for standard targets of the same size. In those cases, work at the upper limit of time range given.


'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. TripleMaster PCR System
2. The TripleMaster PCR System for three different amplifications
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR System

(Date:7/29/2015)... MA (PRWEB) , ... July 29, 2015 , ... ... Sherley says the company is making good progress in evaluating the full range ... adult tissue stem cells. Adult tissue stem cells perform the day-to-day renewal and ...
(Date:7/28/2015)... 2015  PDL BioPharma, Inc. (PDL, or the Company) ... will release its second quarter 2015 financial results for ... 5, 2015, after market close. PDL,s management will host ... p.m. Eastern Time to discuss the financial results.  A ... via the webcast link on the PDL website. ...
(Date:7/28/2015)... DUBLIN , July 21, 2015 ... the addition of the "Biomedical Refrigerators and ... Growth, Trends and Forecast 2014 - 2022" ... global biomedical refrigerators and freezers market has been ... diagnostic centers, blood banks, and others. The others ...
(Date:7/28/2015)... MORRISTOWN, N.J. , July 28, 2015 /PRNewswire/ ... its second Phase 1 clinical trial with MMI-0100, ... that is being developed for pulmonary disorders characterized ... the United Kingdom , is ... study further evaluating the safety and tolerability of ...
Breaking Biology Technology:Asymmetrex Plans to Report Results From Adult Stem Cell Technology Development Milestone Studies At End of Summer 2Asymmetrex Plans to Report Results From Adult Stem Cell Technology Development Milestone Studies At End of Summer 3Asymmetrex Plans to Report Results From Adult Stem Cell Technology Development Milestone Studies At End of Summer 4PDL BioPharma to Announce Second Quarter 2015 Financial Results on August 5, 2015 2Biomedical Refrigerators and Freezers Market Report - Global Industry Analysis 2015 2Biomedical Refrigerators and Freezers Market Report - Global Industry Analysis 2015 3Moerae Initiates Lipopolysaccharide (LPS) Challenge Study of Inhaled MMI-0100 for Pulmonary Disease Following Encouraging Safety and Tolerability Observed in Single Ascending Dose Phase 1 Study 2
... -- Advisor to President Barack Obama for the Office ... visited the Navy,s corporate laboratory, March 16, to dedicate ... (LASR) and tour the sprawling 130-acre Washington, D.C., campus. ... Navy, Marine Corps and our Nation in ever evolving ...
...  Foundation for Health Improvement and Technology (FHIT), ... nonprofit, with the mission to rid the world ... educational programs promoting early detection and aggressive medical ... have made it entirely possible to detect many ...
... 2012More than half of likely voters doubt that the ... in science, technology and health care by the year ... commissioned by Research!America. The findings reveal deep concerns among ... status in innovation, research and development before the next ...
Cached Biology Technology:White House S&T advisor the Honorable Dr. John Holdren tours Naval Research Laboratory 2Foundation for Health Improvement and Technology (FHIT) Launched to Beat Cardiovascular Disease 2More than half of Americans doubt US global leadership in 2020 2
(Date:7/27/2015)... ANGELES , July 27, 2015   ... evidence- and experience-based clinical improvement solutions, today announced ... solution is now available on Android smartphones and ... inpatient and post-care organizations can use ZynxCarebook to ... same patient,s condition, streamline care transitions to other ...
(Date:7/23/2015)... July 23, 2015 Research and Markets ... the "Global Outlook of the Biometrics Industry ... The global biometrics market is likely ... commercial applications owing to the uptake of multiple ... transformation that enhances the growth of biometrics in ...
(Date:7/21/2015)... Connecticut , July 21, 2015  NXT-ID, ... "Company"), a biometric authentication company focused on the ... Wocket® smart wallet, announces that it has filed ... System and Method. This invention ... not only authorizes an account, but also the ...
Breaking Biology News(10 mins):Zynx Health Adds Android Device Support to ZynxCarebook Solution 2Zynx Health Adds Android Device Support to ZynxCarebook Solution 3Global Outlook of the Biometrics Industry 2015 2Global Outlook of the Biometrics Industry 2015 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 2NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 4
... CLEVELAND, Ohio (June 12, 2013)More than half of middle-aged women ... Hispanic women are less likely to have them than white ... high, showed a survey of some 1,500 women published online ... American Menopause Society (NAMS). The survey, conducted by researchers ...
... patent was awarded today to the late Ernest Geskin, PhD, ... a mechanical engineering professor at NJIT for more than 25 ... his former student and now a senior mechanical engineer for ... and Method for Forming of Tubular Parts" discloses a method ...
... study finds that stroke patients, brains show strong cortical motor ... that offers new insight into stroke rehabilitation. Using functional ... monitored the brains of 24 individuals 12 who had ... as they watched others performing actions made using the arm ...
Cached Biology News:Hot flashes before menopause? It can happen 2Patent awarded today to NJIT for novel use of water jets to create high tensile strength alloy parts 2Exercise for stroke patients' brains 2
... stain has bright fluorescence when bound to dsDNA ... for detecting dsDNA in gels using laser scanners ... nucleic gel acid stain has also found ... bandshift assays. One mL stains 100 minigels. A ...
Eagle's Ham's amino acids; EHAA...
... provides quick and easy operation of a ... control. The software is fully secure ... CFR Part 11 regulations, and compatible with ... IRsolutions versatility enables use in high-level research ...
... We can introduce random mutations to the gene ... then cloned into a vector of choice to ... Mutation frequency can be set to a desired ... of clones 106-108. Mutations can be restricted ...
Biology Products: