Navigation Links
TripleMaster PCR System

  • Just before cycling, pipette 10 l of Mastermix 1 and then 40 l of Mastermix 2 into a 0.2 ml PCR tube on ice. Mix gently by pipetting 3-4 times up and down. Do not vortex and/or centrifuge!
  • Place immediately into a thermal cycler which is pre-heated at 93C and start cycling. Examples of cycling profiles for various long targets are given for the Eppendorf Mastercycler (Table 4). For customer-specific targets, only the annealing temperature should be adapted. By changing the time of the elongation step the product yield can be optimized following the rules given in Table 4 for different target sizes and template complexity.
  • Analyze large amplification products on a 0.4 % agarose gel preferably in TAE-buffer using appropriate DNA markers. For separation of products > 25 kb in a standard electrophoresis chamber, a 12-hour run at 40 V is recommended. Generally, efficient separation of very large DNA fragments is achieved only by inverse or pulse-field
    electrophoresis.
  • Table 2: Mastermix composition for long range PCR 1 components included in the TripleMaster PCR System Plus
    2 some applications may require an extra addition of Magnesium Solution (supplied) to set up a final concentration >2.5 mM, e.g. when DNA amounts >0.5 g are used.

    Cycling program for long range PCR

    The complete program for the 40 kb control amplification included in the TripleMaster PCR System Plus on the Eppendorf Mastercycler is given in Table 3. This data should be used as a guide for customized adaptation of the cycling program to an appropriate prim
    '"/>

    Source:


    Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

    Related biology technology :

    1. TripleMaster PCR System
    2. The TripleMaster PCR System for three different amplifications
    3. TripleMaster PCR System: Direct Comparison with Competitors
    4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
    5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
    6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
    7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
    8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
    9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
    10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
    11. Enhanced PCR Cloning System
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: TripleMaster PCR System

    (Date:9/18/2014)... 18, 2014  MEI Pharma, Inc. (Nasdaq: MEIP ... novel therapies for cancer, announced today that Daniel P. ... at BioCentury,s NewsMakers in the Biotech Industry conference ... from the Millennium Broadway Hotel & Conference Center in ... presentation can be accessed at www.meipharma.com . A replay ...
    (Date:9/18/2014)... 18, 2014 OMICS Group International ... to participate in the ‘Open Access Week’ by ... OMICS Group Pharma Journals from 20-26th ... the fastest emerging Sciences, accelerating with a tremendous ... across the globe. The industry is gearing with ...
    (Date:9/18/2014)... USC have developed a new microscopy technology that ... animals at higher-than-ever resolution. , Dubbed "Complementation Activated ... resolutions that are an order of magnitude finer ... the behavior of biomolecules at the nanometer scale. ... by Nature Communications , the researchers behind ...
    (Date:9/18/2014)... City, MX (PRWEB) September 18, 2014 ... in Mexico City, MX. ASEA is the manufacturer of ... and the skin care product, RENU28. , The ... Regional Vice President Duvan Botero, and ASEA Field Leader ... Samuelson holds a PhD in Atomic/Medical Physics from the ...
    Breaking Biology Technology:MEI Pharma To Present At BioCentury's NewsMakers Conference 2MEI Pharma To Present At BioCentury's NewsMakers Conference 3Pharma and Omics Journals to Address Global Challenges during Open Access Week 2Pharma and Omics Journals to Address Global Challenges during Open Access Week 3Scientists pioneer microscopy technique that yields fresh data on muscular dystrophy 2Announcing the ASEA Mexico Grand Opening Tour Beginning September 18th 2
    ... Corporation,(Nasdaq: VOLC ) today announced that it will ... of fiscal 2007 on Tuesday, February 12., The ... financial,results and operating activities open to all interested parties ... Time), Tuesday, February,12, hosted by Scott Huennekens, president and ...
    ... Begin at ProHEALTH Facility by End of First Quarter, ... NBS ),which is pioneering the pre-disease collection, processing ... need, today announced,receipt of a provisional license for comprehensive ... from the New York State,Department of Health for the ...
    ... - Three New Board Members to Form Audit ... 5 /Xinhua-PRNewswire-FirstCall/ --,China Pharma Holdings, Inc. ("China Pharma") ... generic and brand,bio-pharmaceutical products in China, today announced ... Baowen Dong to its Board of Directors,forming the ...
    Cached Biology Technology:Volcano Corporation Schedules Fourth Quarter Conference Call, Webcast 2NeoStem Announces NY State License for Adult Stem Cell Collection 2NeoStem Announces NY State License for Adult Stem Cell Collection 3China Pharma Appoints Three Independent Non-Executive Directors 2China Pharma Appoints Three Independent Non-Executive Directors 3
    (Date:9/18/2014)... up to 400 kilometres in search of a home, which ... Clownfish spend their entire adult lives under the protection of ... open ocean, says study co-author, Dr Hugo Harrison from the ... at James Cook University. , "In the past we haven,t ... rare glimpse into how far they can swim, crossing large ...
    (Date:9/17/2014)... care to reproductive technologies, the justice and well-being ... people to identify key issues, articulate their values ... the most defensible ways forward. But what are ... conversations?, The Hastings Center and the Presidential Commission ... up to publish a series of essays to ...
    (Date:9/17/2014)... the Arts and Humanities has received a $260,000 grant ... project, "The Boundaries of the Human in the Age ... a wide-ranging series of events aimed at exploring two ... artists and researchers who are exploring the boundaries of ... humanism, and the other involves the increasingly influential hypothesis ...
    Breaking Biology News(10 mins):Nemo's epic journey to find a new home 2Why bioethics literacy matters 2Why bioethics literacy matters 3Mellon Foundation awards grant for major project in the humanities and sciences 2
    ... April 22, 2012) Researchers at Columbia University Medical ... the retention and release of the brain,s stem cells. ... neurologic development and could eventually lead to regenerative therapies ... collaborative effort of the laboratories of Drs. Anna Lasorella ...
    ... reductions in salt content and taxation on products containing salt ... each year from cardiovascular disease (CVD) by 2-3 per cent ... World Congress of Cardiology are the first findings from a ... year. The study set out to assess ...
    ... the Florida campus of The Scripps Research Institute has been ... Health to develop a range of new tests that could ... degenerative disorders of advancing age. Shuji Kishi, an assistant ... three-year study. The new tests will focus on diseases ...
    Cached Biology News:'Housekeeping' mechanism for brain stem cells discovered 2'Housekeeping' mechanism for brain stem cells discovered 3Tax on salt could reduce cardiovascular disease deaths by 3 percent 2Researcher awarded $1 million for stress-associated disease and aging research 2
    Rabbit polyclonal to Leucyl tRNA synthetase ( Abpromise for all tested applications). Antigen: L930-H1176 denatured human recombinant LRS. Entrez Gene ID: 3926 Swiss Protein ID: Q9P2J5...
    Neutrophil cytosol factor 2 (NCF-2) (Neutrophil NADPH oxidase factor 2) (67 kDa neutrophil oxidase factor) (p67-phox) (NOXA2). [Source:Uniprot/SWISSPROT;Acc:P19878] Antigen: Recombinant Protein E...
    ... Rabbit polyclonal to FANCM ( Abpromise ... Antigen: Synthetic peptide derived from ... Human FANCM. (Note: the amino acid ... available as ab27787 .) ...
    Rabbit polyclonal to 13,14 dihydro 15 keto Prostaglandin E2 ( Abpromise for all tested applications)....
    Biology Products: