Navigation Links
TripleMaster PCR System

  • Just before cycling, pipette 10 l of Mastermix 1 and then 40 l of Mastermix 2 into a 0.2 ml PCR tube on ice. Mix gently by pipetting 3-4 times up and down. Do not vortex and/or centrifuge!
  • Place immediately into a thermal cycler which is pre-heated at 93C and start cycling. Examples of cycling profiles for various long targets are given for the Eppendorf Mastercycler (Table 4). For customer-specific targets, only the annealing temperature should be adapted. By changing the time of the elongation step the product yield can be optimized following the rules given in Table 4 for different target sizes and template complexity.
  • Analyze large amplification products on a 0.4 % agarose gel preferably in TAE-buffer using appropriate DNA markers. For separation of products > 25 kb in a standard electrophoresis chamber, a 12-hour run at 40 V is recommended. Generally, efficient separation of very large DNA fragments is achieved only by inverse or pulse-field
    electrophoresis.
  • Table 2: Mastermix composition for long range PCR 1 components included in the TripleMaster PCR System Plus
    2 some applications may require an extra addition of Magnesium Solution (supplied) to set up a final concentration >2.5 mM, e.g. when DNA amounts >0.5 g are used.

    Cycling program for long range PCR

    The complete program for the 40 kb control amplification included in the TripleMaster PCR System Plus on the Eppendorf Mastercycler is given in Table 3. This data should be used as a guide for customized adaptation of the cycling program to an appropriate prim
    '"/>

    Source:


    Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

    Related biology technology :

    1. TripleMaster PCR System
    2. The TripleMaster PCR System for three different amplifications
    3. TripleMaster PCR System: Direct Comparison with Competitors
    4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
    5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
    6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
    7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
    8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
    9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
    10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
    11. Enhanced PCR Cloning System
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: TripleMaster PCR System

    (Date:10/20/2014)... 20, 2014 Convey Computer , ... team from Iowa State University won first place in ... a Convey HC-2ex, the team’s solution achieved the highest ... than the second place finisher. , Experts from all ... the month long challenge, using a variety of design ...
    (Date:10/20/2014)... Inc. (NASDAQ: GNVC ) today announced the retirement ... of directors effective on October 24, 2014.   Dr. Horovitz joined ... chairman from June 2006 to November 2013.  During his tenure, ... Audit Committees of the board.  "We deeply ... to GenVec, and its stockholders," said Wayne T. Hockmeyer ...
    (Date:10/20/2014)... OR (PRWEB) October 20, 2014 Local ... participate in an investigational study of donor stem cells ... in stem cell therapy and has performed clinical stem ... this study is to determine if a single injection ... affected joints can help reduce pain and inflammation in ...
    (Date:10/19/2014)... 19, 2014 The Asia-Pacific Bromine ... in Asia-Pacific with analysis and forecast of revenue. ... Pacific Bromine Market report, to get an idea ... a glimpse of the segmentation in the Asia-Pacific ... and figures. , http://www.micromarketmonitor.com/market/asia-pacific-bromine-6741503144.html , ...
    Breaking Biology Technology:Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 2Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 3Zola P. Horovitz To Retire From GenVec Board 2Cascade Veterinary Referral Center Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 3
    ... Strand, founder of StrandWare Inc., has established a ... located in Eau Claire, provides services to companies ... , ,To promote his new company, Strand is ... of clients through March 17, 2003. Charging only ...
    ... a leading developer of K-12 software solutions for ... recently announced their online, ASP software that streamlines ... monitors the progress of children with disabilities. ... Wisconsin school districts during 2003, provides special education ...
    ... looks as if Small Tree Communications might be ... former Cray and SGI employees to brave the market and ... Minn.-based business, with ties to legacy Chippewa Valley computer companies, ... for Apple Computers enterprise customers who need enhanced networking for ...
    Cached Biology Technology:Former SGI/Cray Team Develops Products for G5 Platform 2Former SGI/Cray Team Develops Products for G5 Platform 3
    (Date:10/17/2014)... . Why do ... to reproduce? And why are there two sexes anyway? These ... of the research journal Molecular Human Reproduction published ... Bielefeld University Bielefeld has compiled this special issue on sperm ... to copulate with several males in quick succession – chimpanzees ...
    (Date:10/17/2014)... German . ... When treating overdoses, doctors are often limited to supportive therapy ... is a combination of drugs involved. So what can be ... grandmother,s pills? ETH professor Jean-Christophe Leroux from the Institute of ... to this question. "The task was to develop an agent ...
    (Date:10/16/2014)... at William and Mary,s Virginia Institute of Marine ... the Environmental Protection Agency to identify the streams ... to develop tools to help local governments and ... depends critically on a dataset of tidal-marsh observations ... Only with this historical baseline can today,s researchers ...
    Breaking Biology News(10 mins):Sperm wars 2Emergency aid for overdoses 2Emergency aid for overdoses 3EPA grant will help localities conserve headwater wetlands 2EPA grant will help localities conserve headwater wetlands 3
    ... weaker as they age and then die, but that,s not the ... of aging at all. These are among the findings in ... this week. The study compares the aging patterns of humans ... about aging and what it should be like," said Pedro F. ...
    ... release is available in French . ... by Canadian researchers may pave the way for more effective treatment ... ETMR/ETANTR. The tumour, which is seen only in children under four, ... a new model for how this brain tumour develops and suggests ...
    ... the science behind pain, from a simple "ouch" to the ... But now, researchers are reporting a promising step toward studying ... Journal of the American Chemical Society , scientists describe the ... that could result in better ways to relieve pain and ...
    Cached Biology News:Not all species age the same; humans may be outliers 2Canadian researchers lead groundbreaking discovery in deadly childhood cancer 2Canadian researchers lead groundbreaking discovery in deadly childhood cancer 3
    , Premium quality synthesis and purification , You provide the sequence, we'll do the rest , Rigorous quality control , Your choice of purification method, amount and format , Available annealed...
    ... service provides the premium quality siRNAs you ... siRNAs can be readily ordered online by ... or by providing the sense and antisense ... Silencer Validated and Silencer ...
    ... custom siRNA service provides the premium quality ... research. Custom siRNAs can be readily ordered ... target sequence, or by providing the sense ... Ambion's Silencer Validated and Silencer Pre-designed siRNAs, ...
    Human TGF-beta RIIb Affinity Purified Polyclonal Ab...
    Biology Products: