Navigation Links
TripleMaster PCR System

kb.
- Use thin-walled 0.2 ml PCR tubes.
- Ensure that the 0.2 ml PCR tubes fit exactly into the thermal block of the cycler. Eppendorf 0.2 ml PCR tubes are recommended for the Eppendorf Mastercycler.
- Always prepare two master mixes and chill them on ice.
- Set up the complete reactions on ice.
- Mix the final reaction by gently pipetting up and down (three times). Oil overlay is not necessary for cyclers equipped with heated lids.
- Always use an elongation temperature of 68C and a denaturation temperature of 93C.
- Use a final dNTP concentration of 500 M for targets > 10 kb.
- Primers should have annealing temperatures above 60C.
- Do not apply hot start techniques such as Ampliwax.

Protocol for long range PCR

Prepare two Mastermixes and keep them on ice. Mastermix 1 contains primers and template. Mastermix 2 contains enzyme, dNTPs and buffer. Combine Mastermixes 1 and 2 immediately prior to commencing cycling in order to avoid primer/template degradation by 35 exonuclease activity and unspecific primer extension at lower temperatures.
  1. Thaw the reagents listed in Table 2 carefully and keep on ice.
  2. Make sure reagents are thoroughly thawed. Mix each reagent and briefly centrifuge before use.
  3. If various amounts of the TripleMaster Polymerase Mix are to be tested, prepare an enzyme dilution in 1x Tuning Buffer as described in the Appendix.
  4. Set up Mastermix 1 in a sterile microcentrifuge tube, mix well, centrifuge and place on ice (see Table 2).
  5. Set up Mastermix 2 in a seperate sterile microcentrifuge tube, mix well, centrifuge and place on ice (see Table 2).

  6. '"/>


Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. TripleMaster PCR System
2. The TripleMaster PCR System for three different amplifications
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR System

(Date:7/1/2015)... and BANGALORE, India , July ... profiling company that uses next generation sequencing technology to ... Scott A. Storrer as chief executive officer. In ... will be responsible for all strategy, business expansion and ... who has served as Strand,s chairman and CEO since ...
(Date:7/1/2015)... Ariz. , July 1, 2015 Isagenix ... it has earned three Gold, three Silver, and three ... Awards gala held in Chicago ... as the premier business awards program in the U.S. ... recognizes our company,s strength in developing leaders, cultivating talent, ...
(Date:7/1/2015)... ... July 01, 2015 , ... Apex Therapeutics announced today that company CEO, ... PA. , The presentation took place on Tuesday, June 16 and provided ... development of APX3330 for the treatment of pancreatic cancer. A copy of the ...
(Date:6/30/2015)... -- R-Japan Co.,Ltd. obtained the license of cell processing facility ... the Ministry of Health, Labour and Welfare Kinki Bureau of ... The fact that R- Japan received the ... and Medical Devices Agency (PMDA) on May 18, 2015 two ... cell manufacturing service to medical institutions. As of November 25, ...
Breaking Biology Technology:Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 2Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 3Isagenix Earns Nine American Business Awards 2Isagenix Earns Nine American Business Awards 3Apex Therapeutics Presented Company Overview at This Year’s BIO International Convention in Philadelphia, PA 2R-Japan Obtained Autologous Stem Cell Manufacturing License from the Ministry of Health, Labour and Welfare 2
... Wilmington, DE (PRWEB) May 5, 2009 -- The ... group vice president -- DuPont Applied BioSciences ... the biofuels industry that ranges from the advanced ... the new advanced biofuels technologies we are bringing ...
... Inc., a clinical-stage biopharmaceutical company focused on diseases of ... 1B trial for SQ109 with the dosing of the ... intended to replace one or more of the current ... U.S. FDA Fast Track designation and FDA/EMEA Orphan Drug ...
... Syrinx Consulting Corporation , an IT consulting ... SharePoint applications , announced today that ... a leading biopharmaceutical company in the Boston area. ... gigabytes of data from a SharePoint 2003 environment to ...
Cached Biology Technology:DuPont Statement on Obama Administration Biofuels Interagency Working Group 2DuPont Statement on Obama Administration Biofuels Interagency Working Group 3Sequella Commences Phase 1B Study for New Tuberculosis Drug SQ109 2Syrinx Wins Contract with Leading Pharmaceutical Company for SharePoint 2007 Services 2
(Date:6/17/2015)... GERMANTOWN, Maryland , June 17, 2015 ... Produktlinie mit DNA-Tests verbessert den Analyseprozess und senkt Laborkosten ... Frankfurt Prime Standard: QIA) hat heute in den ... Investigator ® für die forensische ... Kits zur Erstellung genetischer Fingerabdrücke bieten eine integrierte Lösung ...
(Date:6/17/2015)... Calif. , June 17, 2015  Synaptics ... human interface solutions, today announced that Xiaomi, one ... the Synaptics ® ClearPad ® family ... display driver integrated circuits (DDICs) for its latest ... By leveraging ClearPad for full in-cell display solutions ...
(Date:6/16/2015)... June 16, 2015 /CNW Telbec/ - handyem Inc. ... around the incorporation of handyem,s HPC-150 portable flow ... the Mo-POD™. This unprecedented model of mobile laboratory ... at the Pennsylvania Convention ... 15 th to 18 th 2015. ...
Breaking Biology News(10 mins):QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 3QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 4QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 5QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 6Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 2Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 3handyem and FlowMetric Diagnostics announce their partnership in the development of Flow Cytometry- Based Solutions in diagnostic capabilities 2handyem and FlowMetric Diagnostics announce their partnership in the development of Flow Cytometry- Based Solutions in diagnostic capabilities 3
... supply based on biomass grown to generate electricity and ... Jrgen O. Metzger from Carl von Ossietzky University of ... University of Goettingen in Germany, it is both a ... suggests it is unrealistic. Their findings(1) are published online ...
... know that poor diet and lack of exercise can lead ... is not just the heart that is affected blood ... pain when walking, immobility and even in extreme cases, amputation. ... artery disease (PAD) once we are 65 or over, and ...
... sensors for cancer drugs and other DNA-damaging agents inside ... wrapped in DNA, can detect chemotherapy drugs such as ... that damage DNA. "We,ve made a sensor that ... and actually detect several different classes of molecules that ...
Cached Biology News:Turning over a new leaf for future energy supplies 2MIT nanotubes sniff out cancer agents in living cells 2
Form: Ready to use Applications: ELISA...
Form: Ready to use Applications: ELISA...
X-prolyl aminopeptidase (aminopeptidase P) 1, soluble...
2,4,6, Trinitrophenyl hapten is conjugated to lipopolysaccharide (LPS)....
Biology Products: