Navigation Links
TripleMaster PCR System

Each lot of the TripleMaster PCR System is performance-tested in long range PCR. Routinely, TripleMaster PCR System is tested with genomic DNA using specific primers for the human tPA gene to obtain 24 and 27 kb products as well as with DNA and specific primers to amplify a 40 kb product.

8 Buffer choice

Choosing the appropriate reaction buffer is essential for obtaining optimal results with the TripleMaster PCR System. Use Table 1 as a guide for buffer selection in conjunction with the target size and template used. For targets ranging between 2 - 10 kb it is recommended to try both buffers and then choose the one with the best ratio of yield to specificity. For amplification of targets <= 500 bp or those with a low template concentration (< 10 ng/50 l of genomic DNA or < 500 pg/50 l of plasmid DNA) increase the final buffer concentration from 1x to 1.6x by adding 8 l of the 10x buffer to a 50 l assay. The resultant higher ionic strength of the reaction medium forces the TripleMaster Polymerase Mix to amplify short DNA fragments with a higher yield on small amounts of template DNA. Table 1: Buffer selection guide

9 Protocols

9.1 Long range PCR

Amplification with TripleMaster PCR System using the Tuning Buffer is robust and efficient for long targets up to 50 kb, even those where other enzymes fail.
General hints
- Use high molecular weight DNA templates only, stored at 28C. Avoid freezing the template DNA. Check the quality of the DNA; the average size must be > 50
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. TripleMaster PCR System
2. The TripleMaster PCR System for three different amplifications
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR System

(Date:12/22/2014)... (PRWEB) December 22, 2014 The ... global leader of technical training across the life sciences ... Data Management (SCDM) to provide the organization's members ... programs —providing access to the more than 350 sessions ... members with 10% off when registering for a ...
(Date:12/22/2014)... , Dec. 22, 2014 /PRNewswire/ - RepliCel Life Sciences ... medicine company focused on the development of autologous cell ... of the University of Calgary in conjunction with co-authors ... which further validates the company,s ongoing clinical research using ... of pattern baldness. The paper entitled " ...
(Date:12/22/2014)... , Dec. 22, 2014 GenomeDx Biosciences ... Decipher Prostate Cancer Classifier, a genomic test for prostate ... node-negative, high-risk men managed by radical prostatectomy without adjuvant ... prostatectomy is relatively uncommon, using tumor genomics to identify ... metastatic disease is an important advance. The study has ...
(Date:12/22/2014)... The American Journal of ... research, reviews and editorials addressing developments and pathways ... published a provocative article exploring the role of ... potential treatment of prostate cancer. , The ... the possibility that there could be a connection ...
Breaking Biology Technology:CfPIE Announces Partnership with the Society for Clinical Data Management 2CfPIE Announces Partnership with the Society for Clinical Data Management 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 2Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 4Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 5Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 2Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 3Prostate Cancer Experts Dissect the Role of Follicle-stimulating Hormone in the Development, Progression and Potential Treatment of Prostate Cancer 2Prostate Cancer Experts Dissect the Role of Follicle-stimulating Hormone in the Development, Progression and Potential Treatment of Prostate Cancer 3Prostate Cancer Experts Dissect the Role of Follicle-stimulating Hormone in the Development, Progression and Potential Treatment of Prostate Cancer 4
... Wis. -- Northwestern Wisconsin meat snack producer ... Intelligence, Inc. solutions to increase operating efficiencies and ... sells a variety of beef jerky snacks under the ... global meat snacks company. , ,Jack Link's plans ...
... Platypus Technologies of suburban Madison has received ... for $200,000 from the National Cancer Institute. The grant ... rapid quantification of protein activity, useful for cancer research. ... II SBIR grant for $2.14 million from the National ...
... government institutions as a business, with a tight bottom ... schools are facing similar scrutiny. The increased academic expectations ... the federal regulations with which schools must comply. ... budget accountability at a time when school funding and ...
Cached Biology Technology:Looking to software to manage the business of education 2Looking to software to manage the business of education 3Looking to software to manage the business of education 4
(Date:12/17/2014)... FORT BELVOIR, Va. , Dec. 15, 2014 ... its efforts to make it easier to detect ... supply chain. Today the agency started ... marking. The capability will validate the authenticity of ... supply chain. The new quality control measures will ...
(Date:12/17/2014)... -- Valencell, a leader in performance biometric data sensor ... leaders such as Intel, Jabra, Atlas, and Scosche to ... These products will be showcased at CES 2015, taking ... . "Our partners choose PerformTek biometrics, ... with the clinical data to back it up," said ...
(Date:12/17/2014)... DENVER , Dec. 16, 2014  Automation ... is this more evident than at international borders. ... secure document scanners have allowed veteran travelers to ... Automated Passport Control (APC) Kiosks at an increasing ... the globe. According to ...
Breaking Biology News(10 mins):Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2
... Washington, D.C. (January 25, 2011) -- In ... displays, touch panels, electronic ink, and solar cells, ... physicists in Iran have created a spintronic device ... being pursued by the semiconductor and electronics industries ...
... theory of natural selection on the realization that genetic ... Some individuals are better adapted to a given environment ... pass on their genes to future generations. But exactly ... poses somewhat of a puzzle to evolutionary biologists. ...
... Duke University bioengineers have developed a new method ... DNA sequences. These novel sequences of recombinant DNA ... types of drugs and bioengineered tissues. Current methods for ... the researchers said, which has hindered the development of ...
Cached Biology News:Armchair nanoribbons made into spintronic device 2Evolution by mistake 2Evolution by mistake 3Evolution by mistake 4New method for rapidly producing protein-polymers 2
Mouse monoclonal antibody raised against a partial recombinant EOMES. NCBI Entrez Gene ID = EOMES...
...
Rabbit polyclonal to MCP2 ( Abpromise for all tested applications). entrezGeneID: 6355 SwissProtID: P49873...
Mouse monoclonal antibody raised against a partial recombinant AATK. NCBI Entrez Gene ID = AATK...
Biology Products: