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TripleMaster PCR System

ong range PCR applications resulting in robust amplification of genomic targets >20 kb and episomal targets up to 50 kb without organic co-solvents. The HighFidelity Buffer is designed for high fidelity amplifications of smaller targets ranging from 100 bp to 10 kb with genomic templates and up to 15 kb with plasmid or phagemid DNA.

Control reagents are supplied with the TripleMaster PCR System Plus for the amplification of a 40 kb fragment.

Applications include amplification of:
very long targets (genomic > 20 kb; episomal up to 50 kb)
long targets (genomic 10 - 20 kb, episomal 10 - 40 kb)
150 bp 10 kb targets with high fidelity for cloning and sequencing purposes
rare and long cDNA from RT reactions
GC-rich and other difficult templates.

2 Precautions and warnings

Appropriate safety apparel such as lab coat, gloves, and eye protection should be worn. For more information, please consult the appropriate material safety data sheets which are available for this kit on line at www.eppendorf.com/msds

3 Materials

3.1 Materials supplied with the kits: Component 100 U
(50 - 100 reactions)
200 U
(100 - 200 reactions)
500 U
(250 500 reactions)
TripleMaster Polymerase Mix (5U/l) 20 l 40 l 100 l 10x Tuning Buffer with Mg2+ 1.5 ml 1.5 ml 1.5 ml
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