Additional information 11.1 Reaction volume 11.2 Use of PCR additives and co-solvents with TripleMaster PCR System 11.3 Enzyme concentration 11.4 Concentrations of dNTPs and Mg²⁺ 11.5 Dilution of the TripleMaster Polymerase Mix 11.6 Fidelity 11.7 Cloning 11.8 Sequencing 11.9 Primer sequences for long targets

1 Introduction

The TripleMaster PCR System combines a powerful polymerase blend with an innovative two buffer system for efficient amplification of targets ranging from 100 bp up to 50 kb, including GC-rich targets and other difficult templates. The TripleMaster Polymerase Mix is a blend of thermostable DNA polymerases with a processivity-enhancing factor providing both an extremely high extension rate and maximal proofreading assisted fidelity.

Both buffers, the Tuning Buffer and the HighFidelity Buffer, of the TripleMaster PCR System have a unique zwitterionic formulation which improves pH-maintenance at high temperatures (7294C), thus reducing pH-driven template degradation to a minimum. The Tuning Buffer is designed for high fidelity l
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