Navigation Links
TripleMaster PCR System

ogous to PCR products amplified by Taq DNA polymerase. TAcloning is therefore recommended. For blunt-end cloning, the fragments need to be polished by Klenow enzyme or T4 DNA polymerase.

11.8 Sequencing

Sequencing with the TripleMaster Polymerase Mix is not recommended due to the inherent 35 exonuclease activity.

11.9 Primer sequences for long targets

universal forward primer (in the control primer mix) :
5-CTGATGAGTTCGTGTCCGTACAACTGGCGTAATC-3 reverse primers: 40 kb: 5-TAATGCAAACTACGCGCCCTCGTATCACATGG-3 (in the control primer mix) 35 kb: 5-ATTATGTCGGTGATACTTCGTCGCTGTCTC-3 30 kb: 5-GAAAGTTATCGCTAGTCAGTGGCCTGAAGAGACG-3 20 kb: 5-GTGCACCATGCAACATGAATAACAGTGGGTTATC-3 10 kb: 5-ATACGCTGTATTCAGCAACACCGTCAGGAACACG-3 universal forward primer for human tPA gene: 5-CCTTCACTGTCTGCCTAACTCCTTCGTGTGTTCC-3 reverse primer for human tPA gene: 15 kb: 5- ACTGTGCTTCCTGACCCATGGCAGAAGCGCCTTC-3 18 kb: 5-GCAGGGGTGCTGCAGAACTCTGAGCTGTACTTCC-3 22 kb: 5-GATGCGAAACTGAGGCTGGCTGTACTGTCTC-3 24 kb: 5-TGTCTCCAGCACACAGCATGTTGTCGGTGAC-3
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. TripleMaster PCR System
2. The TripleMaster PCR System for three different amplifications
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR System

(Date:3/2/2015)... -- Editor Note: For more information about ... Moments ago, Analysts Review released new research updates concerning several ... SSH ), Synergetics USA (NASDAQ: ... ), Spectranetics (NASDAQ: SPNC ), and Thoratec (NASDAQ: ... platform for investors, to hear about what matters - proudly ...
(Date:3/2/2015)... MIAMI (PRWEB) March 02, 2015 ... a newly formed alliance with Shenzhen HANK Bioengineering ... M.D., Ph.D. The alliance will establish a stem ... Guangdong Province, China in September 2015. , Shenzhen ... by Zhang, an award-winning scientist, microbiologist and virologist, ...
(Date:2/27/2015)... Nova Scotia (PRWEB) February 27, 2015 ... been made aware of a fraudulent press release disseminated ... purporting to be from Immunovaccine. This press release states ... Gilead Science, Inc. The press release did not ... the information contained within the press release. Please ...
(Date:2/27/2015)... Markets ( http://www.researchandmarkets.com/research/p2ddnr/global_market ) has announced the addition ... to their offering. , Global Market Report ... Trypsin globally and regionally ( Europe , ... Latin America etc.). It captures Trypsin ... report focuses on three primary areas; manufacture methods & technology ...
Breaking Biology Technology:Quarterly Report Card of Biotechnology Companies: New Research on Sunshine Heart, Synergetics USA, Syneron Medical, Spectranetics and Thoratec 2Quarterly Report Card of Biotechnology Companies: New Research on Sunshine Heart, Synergetics USA, Syneron Medical, Spectranetics and Thoratec 3Quarterly Report Card of Biotechnology Companies: New Research on Sunshine Heart, Synergetics USA, Syneron Medical, Spectranetics and Thoratec 4Global Stem Cells Group Announces Alliance with HANK Bioengineering Co., Ltd. in Shenzhen, China 2Global Stem Cells Group Announces Alliance with HANK Bioengineering Co., Ltd. in Shenzhen, China 3Immunovaccine Disclaims Hoax Press Release 2Global Market Report of Trypsin 2014-2018 2
... Wednesday, September 26th at 8:30am, NEW YORK, Sept. ... today that it will conduct a conference call,to discuss ... portfolio,products for a territory outside the US. The ... at,8:30am EDT. Michael S. Weiss, Chairman and CEO of ...
... LOD, Israel, Sept. 25 Glycominds Ltd., a ... disease,management of autoimmune diseases and cancer, announced today ... is a new consortium,funded by the Government of ... (CD) and colorectal cancer. The BMP consortium was,approved ...
... Sept. 25 Small Bone Innovations, Inc. (SBi),a single-source ... and joint sector, has introduced its PRECISE SD(TM) Volar ... portfolio. The PRECISE SD plate geometry is based ... series of distal radii in the Hamann Todd,Osteological Collection ...
Cached Biology Technology:Keryx Biopharmaceuticals, Inc. Announces Conference Call to Discuss Strategic Alliance 2Glycominds Joins Biomolecular Photonic (BMP) Consortium to Develop New Molecular Imaging Technique 2Small Bone Innovations Introduces New Distal Radius Plating System to Repair Complex Fractures 2Small Bone Innovations Introduces New Distal Radius Plating System to Repair Complex Fractures 3Small Bone Innovations Introduces New Distal Radius Plating System to Repair Complex Fractures 4
(Date:3/2/2015)... , March 2, 2015  Businesses have a ... protection company Tharon Rankins Enterprises has announced the ... an ultra-safe way for businesses to protect their ... transactions. Beconux is a biometric ... Enterprises. Functioning similarly to an ATM machine, the ...
(Date:2/24/2015)... -- This report analyzes the worldwide markets for Iris Biometrics ... the US, Canada , Japan ... , Middle East , and ... provided for the period 2013 through 2020. Also, a seven-year ... analytics are derived from primary and secondary research. Company profiles ...
(Date:2/23/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD ... authentication company focused on the growing mobile commerce market, ... for DISTRIBUTED VOICE DIRECTED PAYMENT METHOD AND ... new groundbreaking payment method. Payment accounts may be selected ... be accessed if both the speech (the word associated ...
Breaking Biology News(10 mins):Personal Data Protection Company Launches New Product 2World Iris Biometrics Industry 2World Iris Biometrics Industry 3World Iris Biometrics Industry 4World Iris Biometrics Industry 5World Iris Biometrics Industry 6World Iris Biometrics Industry 7World Iris Biometrics Industry 8World Iris Biometrics Industry 9World Iris Biometrics Industry 10World Iris Biometrics Industry 11NXT-ID Patents Voice Directed Payments 2NXT-ID Patents Voice Directed Payments 3
... have uncovered new information that may help guide design ... A new detailed structural analysis of the complex formed ... target provides insight into why this particular antibody is ... thus far. The research is published in the February ...
... and a foe ?approach a plant and communicate with it ... trade nitrogen for food. The foe is a parasite that ... a new finding published in Proceedings of the National Academy ... that the two strangers communicate with the plant in very ...
... common adult leukemia in the United States was last reviewed ... it was seen through the eyes of theories that dated ... three of the world's foremost experts on chronic lymphocytic leukemia ... the scientific community's knowledge of CLL that has occurred over ...
Cached Biology News:Research advances quest for HIV-1 vaccine 2Plants respond similarly to signals from friends, enemies 2Recent breakthroughs in common adult leukemia highlighted in New England Journal of Medicine 2
Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts....
... Anti-β-Tubulin IV Recognizes an epitope ... of β-tubulin isotype IV. No ... is observed. ascites fluid synthetic ... sequence of β-tubulin isotype IV, ...
Porcine Aortic Endothelial Cells (PAOEC) (>500,000 cells)...
Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC) (>500,000 cells)...
Biology Products: