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TripleMaster PCR System: Direct Comparison with Competitors

Competitor AB 3.3X Reaction Buffer (1X)
Eppendorf dNTP Mix (0.5 mM)
MgCl2 (2.2 mM)
Competitor AB Polymerase (2.0 U) Cycling parameters

Initial denaturation
93C 3 min

10 cycles:
93C 15 sec
68C 21 min

8 cycles:
93C 15 sec
68C 27 min
+ 20 sec each cycle

Sample analysis

Of each sample 25% was loaded onto a 0.4% agarose gel. The gel was stained with ethidium bromide and photographed.

Results

The Eppendorf samples clearly outperform the competition. In both the amplification of genomic DNA and Lambda DNA, the Eppendorf polymerase provides consistently high yields. Although competitor R samples perform in both cases, the Eppendorf yields are higher and more consistent. Slight amplification, however minimal, can be seen in competitor T samples. The addition of DMSO does not appear to increase amplification considerably. Competitor AB samples perform poorly. Overall the Eppendorf samples are superior to all competitor products tested.


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