( T ...)TripleMaster PCR System: Direct Comparison with Competitors

T Lanes 1617: Competitor AB Lane M: Lambda Hind III Marker Cycling parameters

Initial denaturation
93C 3 min

10 cycles:
93C 15 sec
68C 27 min

17 cycles:
93C 15 sec
68C 21 min
+ 20 sec each cycle

Lambda DNA amplification

20 ng template was used for each reaction. Primers targeting a 40 kb region of the Lambda DNA were used for all amplification reactions. Reaction components from each manufacturer were used as follows in the concentrations presented in parentheses:

Eppendorf TripleMaster PCR System

10X Tuning Buffer (1X)
Eppendorf dNTP Mix (0.5 mM)
MgCl2 (2.4 mM)
TripleMaster (2.0 U) Competitor R 5X Reaction Buffer (1X)
Eppendorf dNTP Mix (0.5 mM)
MgCl2 (2.4 mM)
Competitor R Polymerase (2.0 U) Competitor T 10X Reaction Buffer (1X)
Eppendorf dNTP Mix (0.5 mM)
MgCl2 (2.4 mM)
Competitor T Polymerase (2.0 U)
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