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TripleMaster PCR System: Direct Comparison with Competitors

Competitor R 5X Reaction Buffer (1X)
Eppendorf dNTP Mix (0.5 mM)
MgCl2 (2.4 mM)
Competitor R Polymerase (2.0 U) Competitor T Samples were processed with and without DMSO
10X Reaction Buffer (1X)
Eppendorf dNTP Mix (0.5 mM)
MgCl2 (2.4 mM)
Competitor T Polymerase (2.0 U)
DMSO (2%) Fig1: 24 kb target (tPA gen) amplification from human genomic DNA Lanes 12: Eppendorf TripleMaster PCR System Lanes 34: Competitor R Lanes 56: Competitor T Lanes 78: Competitor T +2% DMSO Lane M: Lambda Hind III Marker 40 kb target amplification from Lambda DNA Lanes 1011: Eppendorf TripleMaster PCR System Lanes 1213: Competitor R Lanes 14-15: Competitor
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