Navigation Links
TripleMaster PCR System: Direct Comparison with Competitors

Lars E. Peters, Walter Gross, and Jennifer Diers
Eppendorf 5 Prime, Inc., Boulder, CO, USA This experiment was performed to compare the Eppendorf TripleMaster PCR System with the long-range PCR products of leading competitors. Eppendorf was compared to competitors T and R in genomic DNA amplification; and competitors T, R, and AB in Lambda DNA amplification. Very stringent cycling conditions were used for all products in terms of a short extension time and low number of cycles. Please note: The cycling conditions are not according to the manufacturers instructions. These conditions prove the high processivity of the TripleMaster polymerase when tested against competitor products. In order to gain the success seen with the TripleMaster system, usual cycling conditions for competitors products would require much longer extension times and many more cycles.

Experimental Setup

Genomic DNA amplification

Reactions were executed using 200 ng of commercially purchased human gDNA (Roche). Primers targeting a 24 kb region of the human tPA gene were used. Primer sequences for the 24 kb human tPA universal forward and the 24 kb reverse primers may be found in the Eppendorf TripleMaster PCR System manual. Reaction components from each manufacturer were used as follows in the concentrations presented in parentheses:

Eppendorf TripleMaster PCR System

10X Tuning Buffer (1X)
Eppendorf dNTP Mix (0.5 mM)
MgCl2 (2.4 mM)
TripleMaster (2.0 U)
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. TripleMaster PCR System
2. TripleMaster PCR System
3. The TripleMaster PCR System for three different amplifications
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling
8. Counting Colonies or Plaques Directly from Images of Agar Plates
9. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
10. Constructing Directional cDNA Libraries from Limited Amounts of RNA
11. Generate High-Quality, Directional Plasmid cDNA Libraries
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TripleMaster PCR System Direct Comparison with Competitors

(Date:7/30/2014)... CA (PRWEB) July 30, 2014 ... from the U.S. Environmental Protection Agency (EPA). This ... in developmental toxicity models, including tests designed to ... formation and the formation of connections between neurons ... of neurodifferentiation and synapse formation by environmental pollutants ...
(Date:7/30/2014)... Immunology Summit-2014 will ... Investigators, scientists in the field, which includes prominent ... Case Western Reserve University School of Medicine, USA, ... “The effects of recombinant human IL-6 and ... Arya Biragyn, a Senior investigator at National Institute ...
(Date:7/29/2014)... (PRWEB) July 30, 2014 ... and collaboration company, is proud to announce the ... management positions in their Boston, Charleston and Atlanta ... to the increased demand for hosted solutions, collaboration ... efficiency and reduce cost. , SoundConnect was founded ...
(Date:7/29/2014)... NASA technologists have hurdled a number of significant ... already revolutionary observing technology originally created for the ... by Principal Investigator Harvey Moseley, a scientist at ... has demonstrated that electrostatically actuated microshutter arrays ... voltage are as functional as the current ...
Breaking Biology Technology:U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 2U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 3Unveiling Breakthroughs in Clinical & Cellular Immunology 2Unveiling Breakthroughs in Clinical & Cellular Immunology 3SoundConnect Organization and Culture is Evolving 2Revolutionary microshutter technology hurdles significant challenges 2Revolutionary microshutter technology hurdles significant challenges 3Revolutionary microshutter technology hurdles significant challenges 4
... molecules in a living cell is something that has long ... diamond, may well be able to do this and potentially ... for, hard-to-cure diseases such as cancer. In a ... Physics and the German Physical Society,s New Journal of ...
... & Co. Inc., a unit of Oppenheimer Holdings, is pleased ... as Managing Director in the Healthcare group of the Firm,s ... science business. He will report to Brian McCarthy, Managing Director ... 2003, Matthias had worked at JMP Securities, where he was ...
... FREMONT, Calif., May 18, 2011 Biomerix Corporation and ... Biomerix 3D Scaffold , a novel three-dimensional tissue ... the Biomerix 3D Scaffold to research institutions across Europe. ... polycarbonate polyurethane , the Biomerix 3D Scaffold is ...
Cached Biology Technology:'Critical baby step' taken for spying life on a molecular scale 2Biomerix Corporation Reaches European Distribution Agreement with Cellon S.A. 2
(Date:7/30/2014)... shrinking ranges and a changing climate threaten some ... plants, and so conservationists establish seed collections to ... in hopes of preserving some genetic diversity., For ... simple models that offer a one-size-fits-all approach for ... saving 50 seed samples regardless of species, pollination ...
(Date:7/30/2014)... Americans make better decisions about what they eat, the ... significant changes to the Nutrition Facts label found on ... in Chemical & Engineering News (C&EN), the ... the suggested updates and the fight that has ... points out that while the Nutrition Facts label has ...
(Date:7/30/2014)... of Engineering recently received a $330,000 award from ... synthesis of organic nanorods." , According to ... and utilization of functional materials, devices, and systems ... in scaling up nanotechnology is the lack of ... one device. A research team led by Dr. ...
Breaking Biology News(10 mins):Saving seeds the right way can save the world's plants 2NSF grant to Wayne State supports new concept for manufacturing nanoscale devices 2
... ago, a tiny mayfly had a problem. Like most adult mayflies, ... no time to waste. She took her mating flight, got fertilized, and ... got stuck in some oozing tree sap and died, preserved for all ... It was a pretty rude ending to what was already going to ...
... COLUMBUS, Ohio Astronomers, research on celestial bodies ... Ohio State University astronomers are working with medical physicists ... treatment one that is intended to be tougher ... studying how chemical elements emit and absorb radiation inside ...
... Wiley of the National Oceanic and Atmospheric Administration (NOAA) and ... (Volume 148, Nos. 5-6) how humpback whales in the Gulf ... ( Megaptera novaeangliae ) are large baleen whales (up to ... dense concentrations, such as krill or herrings. Humpbacks ...
Cached Biology News:Ancient species of mayfly had short, tragic life 2Astronomers reach for the stars to discover new cancer therapy 2Astronomers reach for the stars to discover new cancer therapy 3Astronomers reach for the stars to discover new cancer therapy 4Humpback whales catch prey with bubble-nets 2
Transcription factor Sp2. [Source:Uniprot/SWISSPROT;Acc:Q02086] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
Rabbit Anti-melatonin (different linkage) Polyclonal Antibody...
... [EP282Y] to Asparagine synthetase ( ... applications). Antigen: ... residues in the C-terminus of ... Entrez GeneID: 440 ...
Rabbit Anti-melatonin (different linkage) Polyclonal Antibody...
Biology Products: