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Translate More Protein with Higher Biological Activity

her unrelated proteins. Figure 5 demonstrates that a range of both prokaryotic (GroES, CAT, methionyl-tRNA transformylase (MTF), alkaline phosphatase (AP), SrmB, DEAD, and -gal) and eukaryotic (Green Fluorescent Protein (GFP) and Rhodanese (Rho)) proteins ranging in size from 10-120 kDa can be successfully synthesized using the ActivePro In Vitro Translation Kit.


Easy to Use
ActivePro is easy to use. You simply provide a labeled amino acid (optional) and a DNA template that contains your gene of interest, a T7 promoter, ribosome binding site, and T7 terminator. The DNA template can be easily constructed by inserting your gene of interest into the multiple cloning site of Ambion's control plasmid included in the ActivePro Kit. Simply premix the reagents included in the kit and add your DNA template (plasmid or PCR product) and a labeled amino acid if desired, and incubate at 37C for 1 hour. The resulting biologically active protein is then ready for further functional analysis.


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Cat# Product Name Size 1295 ActivePro In Vitro Translation Kit 25 rxns
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Kit includes your choice of reagents for labeling 3 x 10 9 cells and EasySep magnet....
Plasmid expressing PLAP reporter gene....
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