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Translate More Protein with Higher Biological Activity

monstrates the higher biological activity of both luciferase and -galactosidase when expressed with ActivePro as compared to a coupled retic system. Similar results were obtained with other proteins tested (data not shown).

Figure 1. The ActivePro Kit vs. Coupled Reticulocyte Lysate Kit . DNA templates (1-2 g) encoding several prokaryotic and eukaryotic (*) proteins were in vitro transcribed/translated using 20 Ci 35S-labeled methionine for 1 hour at 37C according to the ActivePro protocol and for 1.5 hour with the retic. 35S- methionine was measured by TCA precipitation and the total protein yields were calculated.

Figure 2. Biological Activities of Proteins Produced Using ActivePro vs. a Coupled Retic Kit. DNA template (1 g) for -galactosidase (A) or luciferase (B) was used in either an ActivePro Kit reaction or a commercially available coupled retic kit according to protocol. Each reaction included 20 Ci 35S-methionine. Each reaction (1 l) was assayed for -gal by colorimetric ONGP hydrolysis or for luciferase by luminescence.


Higher Biological Activity than Other E.Coli Lysate Systems
Often a translation system produces high levels of protein with only a fraction being functionally active. Since many applications require functional protein, biological activity is often the most important characteristic of the synthesized product. The ActivePro lysate and Activity Enhancer reagent (included in the kit) were specifically developed to increase protein stability and enhance proper folding, resulting in more active prot
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