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Translate More Protein with Higher Biological Activity

to the mRNA improves transcript stability and promotes efficient translation. In Ambion's new ActivePro In Vitro Translation Kit, transcription and translation are likewise coupled and require only a DNA template (either prokaryotic or eukaryotic) with the proper promoter and ribosomal binding sequences. This kit has been engineered to produce both high yields of protein and high levels of functionally active protein from both eukaryotic and prokaryotic derived sequences.


Better Yields and Faster Translation than Reticulocyte Lysate Systems
ActivePro synthesizes protein at a very high rate (several hundred microgram per hour per ml of reaction) with the reaction 80% complete in just 1 hour. In contrast, typical coupled retic kits require 1.5 hours longer incubation. Other major disadvantages of reticulocyte based cell-free systems are their inability to provide preparative amounts of synthesized protein, large amounts of lysates being more expensive and also the need for acute manipulation of animals.

ActivePro's E.coli based highly active lysate, an efficient energy regeneration system, and a large supply of amino acids support faster protein synthesis and higher yields. As seen in Figure 1, translation of a sequence with ActivePro typically yields greater than 1000 fold more protein than with a commercially available coupled retic lysate kit. ActivePro also produces proteins that have significantly higher biological activity than a coupled retic system. Figure 2 de
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