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Translate High Yields of Biologically Active Proteins

ce assay (Sigma) in the presence of 1 mM ADP [2]. Carbonic anhydrase 2 (CA2): Esterase activity of CA2 was monitored by the net increase in absorbance at 348 nm in a p-nitrophenyl acetate hydrolysis assay [3]. Caspase 3 (CASP3): Catalytic activity was determined by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC for 1 hr in a 96 well plate reader (400 nm excitation wavelength, 505 nm emission wavelength). Casein kinase 1 (CK1): Activity was determined by phosphorylation of casein kinase 1 substrate peptide (Upstate).


Summary
ActivePro is a superior, cell-free in vitro translation kit delivering both high yields and active protein. It is fast, easy to use, and scalableas much as 1 mg of protein can be synthesized in just 1 hour. Compared to similar kits, ActivePro consistently expresses more biologically active protein in extremely high yields. Because it does not require special equipment, it is the fastest and most economical kit for producing milligram quantities of protein suitable for any downstream application.


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Ordering Information
Cat# Product Name Size 1295 ActivePro In Vitro Translation Kit 25 rxns
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