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Translate High Yields of Biologically Active Proteins

ActivePro In Vitro Translation Kit


Cell-free translation systems are a convenient method for synthesizing proteins in vitro. The major advantages of these in vitro translation systems over conventional in vivo systems are greater flexibility, faster reaction times, and less labor-intensive protocols.

Ambions ActivePro In Vitro Translation Kit is a cell-free, E. coli based translation kit that excels at expressing a wide range of functional proteins for a variety of downstream applications. ActivePro generates high yields of proteins with higher biological activity compared to other commercially available kits. This report demonstrates that a number of complex human proteins can be efficiently translated using this technology.


Performance with Human Templates
Thirteen human full length, open reading frame (ORF) clones were selected from the NIH Mammalian Gene Collection (MGC). The corresponding full length sequences were subcloned into Ambions optimized translation vector (included in the ActivePro Kit) and sequences were confirmed. 12 g each of the 13 constructs were translated in a standard 50 l ActivePro reaction. Each reaction included 35S-labeled methionine and was incubated for 1 hour at 37C. Protein yields, as determined by TCA precipitation, were calculated based on the number of labeled methionines in each sequen
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