Navigation Links
Translate High Yields of Biologically Active Proteins

ActivePro In Vitro Translation Kit


Cell-free translation systems are a convenient method for synthesizing proteins in vitro. The major advantages of these in vitro translation systems over conventional in vivo systems are greater flexibility, faster reaction times, and less labor-intensive protocols.

Ambions ActivePro In Vitro Translation Kit is a cell-free, E. coli based translation kit that excels at expressing a wide range of functional proteins for a variety of downstream applications. ActivePro generates high yields of proteins with higher biological activity compared to other commercially available kits. This report demonstrates that a number of complex human proteins can be efficiently translated using this technology.


Performance with Human Templates
Thirteen human full length, open reading frame (ORF) clones were selected from the NIH Mammalian Gene Collection (MGC). The corresponding full length sequences were subcloned into Ambions optimized translation vector (included in the ActivePro Kit) and sequences were confirmed. 12 g each of the 13 constructs were translated in a standard 50 l ActivePro reaction. Each reaction included 35S-labeled methionine and was incubated for 1 hour at 37C. Protein yields, as determined by TCA precipitation, were calculated based on the number of labeled methionines in each sequen
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Translate High Yields of Active Protein
2. Translate More Protein with Higher Biological Activity
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. ArrayScript RT: Maximize Yields of Full-length cDNA
5. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
6. Synthesize High Yields of Biotinylated aRNA
7. Production of Full-Length, Biologically Active MEK1WT and MEK1CA
8. Highly Active and Removable AffinityTagged Lambda Phosphatase
9. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
10. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
11. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Translate High Yields Biologically Active Proteins

(Date:6/1/2015)... , June 1, 2015 GenomeDx ... genomic analysis platform, successfully classified various subtypes ... expression of certain biomarkers, including one type ... this unique genomic signature has potential as ... neoadjuvant chemotherapy in patients with muscle-invasive bladder ...
(Date:6/1/2015)... June 1, 2015  The Val Skinner Foundation is ... science curriculum for high school students focused on cancer ... annual LIFE Event (LPGA Pros in the Fight ... Club in West Caldwell, N.J. ... stars raised more than $500,000 on Monday, bringing the ...
(Date:6/1/2015)... Mich. , June 1, 2015  Neogen Corporation (NASDAQ: ... assets of Sterling Test House, a leading commercial food testing ... that the acquired laboratory will serve as a base for ... Sterling Test House was incorporated ... all of the food safety and water quality testing for ...
(Date:6/1/2015)... , June 1, 2015   Epic Sciences, Inc. ... products and offerings to personalize and advance the treatment ... expanded the available credit under its existing credit facility ... agreement increases the available capacity of the facility from ... maturity by 27 months to April 1, 2019.  The ...
Breaking Biology Technology:GenomeDx's Decipher Platform Validates Genomic Signature that may Predict Resistance to Chemotherapy in Bladder Cancer Patients in Study Presented at ASCO 2015 Annual Meeting 2GenomeDx's Decipher Platform Validates Genomic Signature that may Predict Resistance to Chemotherapy in Bladder Cancer Patients in Study Presented at ASCO 2015 Annual Meeting 3GenomeDx's Decipher Platform Validates Genomic Signature that may Predict Resistance to Chemotherapy in Bladder Cancer Patients in Study Presented at ASCO 2015 Annual Meeting 4Val Skinner Foundation Announces Partnership With Discovery Education 2Neogen acquires India food safety company 2Epic Sciences Expands Silicon Valley Bank Credit Facility 2Epic Sciences Expands Silicon Valley Bank Credit Facility 3
... Sept. 1 /PRNewswire-Asia-FirstCall/ -- China Kangtai Cactus,Biotech Inc. ... manufacturer and marketer of a variety of cactus-based ... successfully completed the trial,production of cactus-based cigarettes and ... in 2009: (i) low nicotine and (ii) zero ...
... ANNAPOLIS, Md., Sept. 1 PharmAthene, Inc. (NYSE Amex: ... against biological and chemical threats, announced today that management will ... 2009. The Company is scheduled to present on Thursday, ... Salon (5th Floor) at the New York Palace Hotel, New ...
... FORT LAUDERDALE, Fla., Sept. 1 OmniComm Systems, Inc. (OTC ... data capture (EDC) solutions for clinical trials, and ... announced today that three additional clinical research organizations ... the CRO Preferred Program. The partnership allows CROs in the ...
Cached Biology Technology:China Kangtai Cactus Biotech to Launch Patented Cactus-based Low Tar, Low or Zero Nicotine Cigarettes 2China Kangtai Cactus Biotech to Launch Patented Cactus-based Low Tar, Low or Zero Nicotine Cigarettes 3OmniComm Systems Inc. Expands Its Successful CRO Preferred Program(TM) By Signing On Three New CRO Partners 2OmniComm Systems Inc. Expands Its Successful CRO Preferred Program(TM) By Signing On Three New CRO Partners 3
(Date:5/28/2015)... Va. , May 28, 2015 ... release of a new report titled "Markets for ... (reversible polymers, inorganic capsule and vascular systems, biomaterials, ... will grow to around $2.7 billion by 2020. ... from n-tech,s extensive coverage of smart materials. Other recent ...
(Date:5/25/2015)... 2015  Australia,s market for wearable technologies is considered ... the advent of several trials and prototypes. The release ... expected to ignite interest in wearables as well as ... . This in turn will spur greater innovation ... partnerships amongst vendors and distributors. Photo ...
(Date:5/21/2015)... May 21, 2015 According ... Market by Solutions (Hardware, Software, Services), by Applications (Surveillance ... others), by End-Users (Military & Defense & Commercial) ... Thermal Imaging Market is expected to grow from ... 2020, at a Compound Annual Growth Rate (CAGR) ...
Breaking Biology News(10 mins):The Market for Self-Healing Materials to Reach $2.7 Billion by 2020 Says New n-tech Research Study 2The Market for Self-Healing Materials to Reach $2.7 Billion by 2020 Says New n-tech Research Study 3Australian wearables market to grow at CAGR of 78%; revenue of A$1 billion by 2018, says Frost & Sullivan 2Australian wearables market to grow at CAGR of 78%; revenue of A$1 billion by 2018, says Frost & Sullivan 3Australian wearables market to grow at CAGR of 78%; revenue of A$1 billion by 2018, says Frost & Sullivan 4Thermal Imaging Market Worth $9,998.9 Million by 2020 2Thermal Imaging Market Worth $9,998.9 Million by 2020 3Thermal Imaging Market Worth $9,998.9 Million by 2020 4
... STANFORD, Calif. - Life is almost unbearably complex. Humans ... off in dizzying combinations and sequences during their relentless ... understand the machinations of the proteins behind the genomic ... disposal, courtesy of researchers at the Stanford University School ...
... nearly 1.5 million Americans have been diagnosed with autism spectrum ... to have some form of autism, according to the latest ... (CDC). Find out what new research and treatment plans are ... over the past few decades at a panel discussion at ...
... Docosahexaenoic acid (DHA), an omega-3 fatty acid, is essential ... premature infants may be deficient in DHA because they ... the fatty acid accumulates in tissues. In addition, their ... the risk of malnutrition. Furthermore, breastfeeding mothers, ...
Cached Biology News:New Stanford tool enables wider analyses of genome 'deep sequencing' 2New Stanford tool enables wider analyses of genome 'deep sequencing' 3Expert panel to discuss autism spectrum disorders 2Giving DHA supplements to breastfeeding mothers 2
... ( Abpromise for all tested ... Synthetic peptide: IGPEVPDDRDFEPS conjugated to KLH, ... Human Decorin (fragment 36-49 of the ... Entrez GeneID: 1634 ...
GLP expression profiling service on the Affymetrix platform. Starting material can be blood, tissue or RNA. 5 day turnaround. Standard and custom post analyses of microarray data available....
... a fluorescent microscopy-based imaging system that accurately ... automatically calculates cell viability, in a single ... the variability associated with the subjective interpretation ... the EasyCount ViaSure Kit, up to six ...
In Western blotting antibodies recognize 94 K protein band, which corresponds to S-layer protein...
Biology Products: