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Translate High Yields of Active Protein

dase by colorimetric ONGP hydrolysis assay


Human Protein Expression
ActivePro's versatility with a wide range of proteins is further demonstrated by the expression of human templates--Caspase3, MAPK9, and Casein Kinase. The results from expressing these human proteins using the ActivePro Kit are shown in Figure 3.

Figure 3. Complex Human Proteins are Translated With the Same Yield and Efficiency as other Types . (A) DNA templates (1-2 g) for three human proteins were in vitro transcribed/translated using 20 Ci 35S-labeled methionine for 1 hour at 37C according to the ActivePro protocol. 5 l of each reaction were assessed by SDS-PAGE gel and phosphorimaging. (B) Table summarizing data on protein expression yields (calculated as described in Figure 2).


Make the Switch Today
Whether you are currently using an E. coli or a reticulocyte lysate translation system, making the switch to Ambion's ActivePro In Vitro Translation Kit can give you higher yields of more active protein in the shortest amount of time.


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Ordering Information
Cat# Product Name Size 1295 ActivePro In Vitro Translation Kit 25 rxns
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