Navigation Links
Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

James R. Wong, Ph.D. and Wei-Yu Lin, Bio-Rad Laboratories


Summary
A double pulse format for electroporation of mammalian tissue culture cells gave lower transient expression than a single exponential decay pulse. Moreover, separate administration of the two components of the double pulse revealed that transformation was due almost exclusively to the first, high voltage pulse and not to the second, long duration waveform.


Introduction
The Twin-pulse wave delivery is a novel electroporation waveform consisting of a short duration, exponential decay pulse followed by a second capacitor discharge yielding a long duration pulse. It is claimed that the first high-voltage pulse creates pores, or openings in the cell walls due to the high voltage delivered... (and) the second low-voltage pulse delivers energy to those cells shocked by the first pulse, therefore favoring entry of exogenous molecules into the cells by means of creating an active electrophoresis field.1 While the transient formation of pores in the plasma membrane following delivery of appropriate electrical fields has been documented via electron microscopy,2 the mechanism of entry and egress of materials through these pores is not known, and no evidence has been provided for the postulated Twin-pulse description.

De Chasseval and de Villartay described the use of double electroporation pulse format to introduce pairs of plasmids into human lymphoid cells.3 Their principal finding was that cotransfection of a plasmid encoding the SV40 large T antigen with a reporter plasmid bearing the SV40 origin of replication increased transient lucifer
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
3. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
9. A New Lambda Vector for Mammalian Expression
10. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
11. Codon Bias-Adjusted BL21 Derivatives for Protein Expression
Post Your Comments:
(Date:4/24/2015)... PAREXEL International Corporation (NASDAQ: PRXL ), ... Partnerships in Clinical Trials named PAREXEL,s Drew Garty ... of the Year.  The honor recognizes Mr. Garty,s leadership ... MyTrials ® Data-Driven Monitoring solution. ... Partnership Awards in Boston , Mr. ...
(Date:4/23/2015)... 2015 The Thomas Brain Health ... a groundbreaking pilot study to see if early ... as little as three to six months. , ... nutritional medicine—will aggressively target and simultaneously treat the ... aging brain. These include oxidative stress, neuroinflammation, mitochondrial ...
(Date:4/23/2015)...  Neurocrine Biosciences, Inc. (Nasdaq: NBIX ) announced ... 2015 results before the Nasdaq market opens on Thursday, ... call and webcast to discuss its financial results and ... at 8:00 a.m. Eastern Time (5:00 a.m. Pacific Time). ... by dialing 866-952-1906 (US) or 785-424-1825 (International) using the ...
(Date:4/23/2015)... 2015  Veracyte, Inc. (NASDAQ: VCYT ) today ... to sell approximately $40 million of its common stock ... will purchase an aggregate of 4,907,975 shares of common ... the closing stock price on April 22, 2015. The ... including Broadfin Capital, Camber Capital, Deerfield, Longwood Capital Partners ...
Breaking Biology Technology:PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 2PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 3PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 4PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 5New Study Looks to Reverse Alzheimer’s Disease 2New Study Looks to Reverse Alzheimer’s Disease 3Neurocrine Biosciences Announces Conference Call and Webcast to Report First Quarter 2015 Results 2Veracyte, Inc. Announces $40 Million Private Placement 2Veracyte, Inc. Announces $40 Million Private Placement 3Veracyte, Inc. Announces $40 Million Private Placement 4
... DIEGO, Feb. 6 SGX Pharmaceuticals, Inc.,(Nasdaq: SGXP ) announced ... The conference information is as follows:, -- 10th Annual ... at 1:15 PM Eastern Standard Time at the Waldorf=Astoria, ... Conference: Tuesday, February 19, 2008 at 11:30 ...
... 5 BioSpecifics,Technologies Corp. (OTC Bulletin Board: BSTC) announced ... owed to it by its former CEO, Edwin H.,Wegman, ... Edwin H. Wegman,repaid the loan amount plus accrued interest ... of BioSpecifics, common stock to certain private,investors. The Estate ...
... Facility Services(R), LLC and,its family of companies ... the largest independent clinical technology maintenance and,management ... acquisition,is part of an aggressive growth strategy ... and accelerate the expansion of its,geographic footprint., ...
Cached Biology Technology:Linc Facility Services Acquires Morse Medical 2
(Date:4/13/2015)... 13, 2015 According to ... Biometrics Market Forecast & Opportunities, 2020", the global biometrics ... around 14% till 2020. The biometrics market is ... implementation and review of biometric management systems. The ... products with greater efficiency, are resulting in increasing ...
(Date:4/10/2015)... , April 10, 2015 ... addition of the "Security Competitive Profiles - NEC" ... http://photos.prnewswire.com/prnh/20130307/600769) , NEC will continue to supply ... to the market, with a company focus on the ... anticipated. Winning opportunities in the ...
(Date:4/8/2015)... April 8, 2015  Infinisource, a leading provider ... and Morpho, a leading supplier of biometric identification ... the market,s most advanced biometrically-enabled time clock, the ... time clock is setting a bold, new standard ... for the small and mid-size employer. When connected ...
Breaking Biology News(10 mins):Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2NEC Security Competitive Profile 2015 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 3
... WEST LAFAYETTE, Ind. - Bacteria are able to build camouflaged ... by manipulating a natural cellular process. , ... a pair of proteins from the bacteria Legionella pneumophila , ... divert raw materials within the cell for use in building and ...
... from LIFE the Faculty of Life Sciences at the ... different growth pattern than non-breastfed children. Breastfeeding lowers the levels ... which means that growth is slightly slower. This is believed ... life. The PhD project is part of SKOT, ...
... camera trap video footage from the forests of Thailand ... country are paying off, according to the Wildlife Conservation ... several locations across Thailand,s Western Forest Complex over the ... the lives of tigers, Asian elephants, gaurs, sun bears, ...
Cached Biology News:Purdue scientists reveal how bacteria build homes inside healthy cells 2Purdue scientists reveal how bacteria build homes inside healthy cells 3Breastfeeding promotes healthy growth 2Candid video clips from Thailand show anti-poaching efforts saving wildlife 2
... The micro 1 is a dramatic breakthrough in ... 1mL with 10nL resolution, the micro 1 has ... dispenser. And, the micro 1 never needs re-calibration ... Touch Screen programming on an LCD Display , ...
... series embodies all aspects of classical microscopy ... powerful Achromatic Super Contrast (ASC) objectives coupled ... this microscope combines flexibility and professional microscopy ... B1 series, the DM-B1 steps into the ...
... to Rad51 ( Abpromise for ... Antigen: Synthetic peptide: VEEESFGPQPISRLE conjugated ... amino acids 15-29 of Human Rad51 ... 5888 Swiss ...
... Dye Primer Manual Cycle Sequencing Kit is ... primers and high-resolution fluorescence scanners. Sequencing reactions ... slab gel electrophoresis and scanning of the ... The kit components have been optimized ...
Biology Products: